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Volume 53 (1978)
- Issue 6 Pages 387-
- Issue 5 Pages 317-
- Issue 4 Pages 227-
- Issue 3 Pages 147-
- Issue 2 Pages 77-
- Issue 1 Pages 1-
Volume 53, Issue 2
Displaying 1-8 of 8 articles from this issue
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HISAKAZU OGURA1978 Volume 53 Issue 2 Pages 77-90
Published: 1978
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSA regenerated plant of chromosomally chimeric character was obtained from callus cultures of a variety in Nicotiana tabacum. The chimerism was observed in the progeny plants, at least through three selfed generations. Cytological observations of rooted cuttings from axillary buds or stem tips of this lineage confirmed the chimerism. They were weak in growth vigor and showed low seed-set. The capsules of this line were more variable in size and were usually smaller than those of normal plants. Some were “chimeric” for leaf shape, i.e., a mixing of round and nearly normal leaves was sometimes found within the individual. In F1s resulting from reciprocal crosses between chimeric and normal plants, all were chimeric, except for one plant. Segregation of chimeric vs. stable plants in the F2 and B1 generations can be interpreted to fit for 3:1 and 1:1 ratios, respectively, except for one B1 combination. On the basis of segregation data in F2s and B1s, the mode of inheritance can be explained by assuming that the chimerism is mainly controlled by a simple Mendelian gene, probably induced during tissus culture processes. Another interpretation of the results is also presented. Inheritance of two foliar characters, namely bilateral asymmetry and foliar ratio was also investigated in relation to chromosomal chimerism. The chromosomally unstable line exhibited larger asymmetry indices than the normal line, however, no significant difference was found on foliar ratio between these lines. The differences calculated from the pooled data between chromosomally chimeric and stable plants in one F2 and two B1 lines were significant in bilaterial asymmetry, but not in foliar ratio. The results suggest that bilateral asymmetry of tobacco plants is correlated with the chromosomal chimerism.View full abstractDownload PDF (3087K) -
YOSHIKAZU SADO, SAMUEL H. HORI1978 Volume 53 Issue 2 Pages 91-102
Published: 1978
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSRabbit antisera against crucian carp glucose 6-phosphate dehydrogenase were prepared and their relative inhibition potencies were examined against glucose 6-phosphate Table 2. Michaelis constants of G6PD from various species dehydrogenases from 23 species of teleosts, 5 species of other vertebrates and 8 species of invertebrates. The results demonstrated that the immunological relatedness of teleosts expressed as the relative inhibition potencies of antibody against the enzymes is consistent with the phyletic relationships of teleosts depicted by Greenwood et al. (1966); i.e., the inhibition potencies were: carp, 0.78; dace, 0.69; loaches, 0.38; three species of salmoni-formes, 0.19; eels 0.08.
The inhibition potencies of vertebrates other than teleosts and of invertebrates ranged from 0.0008 to 0.0012, and 0 to 0.0004, respectively. Ascarid and planarian enzymes were not inhibited by any antiserum tested. Electrophoretic examination of the mixture of these enzymes and antiboby revealed, however, that the enzymes do possess at least one antigenic determinant common to crucian carp enzyme. No immunological similarity was found between yeast and crucian carp glucose 6-phosphate dehydrogenases and between glucose 6-phosphate dehydrogenase and hexose 6-phosphate dehydrogenase of crucian carp.View full abstractDownload PDF (1600K) -
YASUYUKI KONDO1978 Volume 53 Issue 2 Pages 103-110
Published: 1978
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSSeveral early-gastrula nuclei from Rana nigromaculata, R. brevipoda, R. japonica, R. rugosa, Hyla arborea japonica, Bombina orientalis, Xenopus laevis or Cynops pyrrhogaster were transplanted into an unfertilized or enucleated R. nigromaculata egg. Immediately after the nuclear transplantation, 3H-thymidine was injected into these eggs. The behavior of the transplanted nuclei and the incorporation of 3H-thymidine by them were examined.
All the transplanted nuclei swelled remarkably in a great majority of the eggs transplanted with nuclei from each of the four Rana species or Hyla as well as in a small number of eggs transplanted with Bombina or Xenopus nuclei. There was a mixture of swollen and degenerative nuclei in a small number of eggs transplanted with Rana or Hyla nuclei as well as in many eggs transplanted with Bombina or Xenopus nuclei. In a small number of eggs transplanted with Bombina or Xenopus nuclei as well as in all the eggs transplanted with Cynops nuclei, the transplanted nuclei were all degenerative.
No remarkable difference was found in the amount of 3H-thymidine incorporation by each nucleus between swollen nuclei from different species.View full abstractDownload PDF (1745K) -
SUSUMU TAKAYAMA, SHUN-ICHI SAKANISHI1978 Volume 53 Issue 2 Pages 111-116
Published: 1978
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSChinese hamster strain cells cultured with 20μM BrdU for two or three rounds of replication were routinely air-dried. The chromosome preparations were 1) treated with 0.001% trypsin at room temperature for 5 to 10s, or 2) incubated in 0.1M Na2HPO4 at 37°C for 2 to 4h. Then, these preparations were stained with alkaline Giemsa
solution (1.5% in 0.1M Na2HPO4) for 5 to 10min. These procedures resulted in a reverse type of sister chromatid differential staining; darkly stained BB-chromatids and faintly stained TB-chromatids.View full abstractDownload PDF (1900K) -
TOSHIHIKO OKADA, KAZUKO KITAMOTO, KIWAMU KAWAKAMI, KAZUKO KANAYAMA1978 Volume 53 Issue 2 Pages 117-127
Published: 1978
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSRibonucleosides (inosine, adenosine, guanosine, cytidine, and uridine) or deoxyribonucleosides (deoxyadenosine, deoxyguanosine, deoxycytidine, deoxyuridine, and thymidine) themselves did not repress β-galactosidase formation in Escherichia coli K12 strain 3000 and other derivative strains but all these nucleosides except guanosine and deoxyguanosine enhanced the repression by glucose or glycerol. Adenosine 3', 5'-cyclic monophosphate reversed the effects of glucose, and of glucose (or glycerol) plus adenosine or cytidine on β-galactosidase synthesis. Neither ribose, deoxyribose nor any of the nucleic acid bases tested except hypoxanthine and adenine repressed the synthesis of β-galactosidase or enhanced the repression by glucose or glycerol. The effects of the nucleosides were dependent on their concentrations and, in the presence of 25mM glucose or glycerol, adenosine and cytidine caused maximal repression at concentrations of 0.2mM or more. Adenosine (0.4mM) exerted its maximal effect in the presence of the concentration of glucose or glycerol which alone caused maximal repression (0.5mM glucose or 0.37mM glycerol). In the absence of inducer, adenosine (0.4mM) also enhanced the effect of glucose on β-galactosidase synthesis in I- constitutive strains.View full abstractDownload PDF (954K) -
TAKUMA GAMO1978 Volume 53 Issue 2 Pages 129-131
Published: 1978
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSDownload PDF (455K) -
HIKOYUKI YAMAGUCHI, TADAO NAITO, ATSUSHI TATARA1978 Volume 53 Issue 2 Pages 133-135
Published: 1978
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSDownload PDF (237K) -
MICHIRU MIZUNO, KIYOSHI SUSUKI1978 Volume 53 Issue 2 Pages 137-142
Published: 1978
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSA strain difference was found in the pancreatic α-amylase in rat strains, Rattus norvegicus. This variation is under the genetic control of a single locus, Amy-1. The locus Amy-1 contains dominant and recessive alleles, Amy-1a and Amy-1b, which correspond to the positive and negative expression of the minor band, respectively. An electrophoretic survey of small intestine esterases showed a new phenotype controlled by Es-3 locus. Linkage tests showed 8.5% recombination frequency between Es-1 and Es-3 loci on linkage group V. Amy-1 and Es-1 loci did not belong to the same linkage group.View full abstractDownload PDF (739K)
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