1971年 Mariat により Ceratocystis stenoceras が S. schenckii の完全世代であるという仮説が提唱されて以来, Ceratocystis 属と S. schenckii の異同に関する研究が盛んに行なわれるようになつた. 今回, 我々は S. schenckii 患者株並びに自然株と C. stenoceras 及び C. ulmi の二株の Ceratocystis 属について形態学的所見並びに免疫蛍光染色法を用いて両者の抗原性状を比較検討した. その結果, Ceratocystis 属の両菌は形態学的に S. schenckii に極めて類似していた. 又 S. schenckii 患者株並びに自然株に対する抗血清及び吸収抗血清を用いた免疫蛍光染色の所見より, 両菌の抗原性状が類似し, Ceratocystis 属は S. schenckii の患者株に近いことが示唆され, Mariat の仮説を支持するデータが得られた. 今後, S. schenckii の側からの有性世代の発見が必要と思われる.

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A bacteriolytic enzyme obtained from the culture filtrate of Aspergillus melleus was purified by disc electrophoresis and examined for its properties enzymologically and immunologically.
The enzyme was a DFP-sensitive alkaline protease, having residues of either tryptophane, tyrosine, or histidine. Neither metal chelating agents nor thiol poisons inhibited the activity of this proteolytic enzyme.
From experiments concerning the enzymatic inhibition using either bacterial cells or pure casein as the substrates, it was suggested that the bacteriolytic activity of this enzyme was based on its nature to hydrolyze the peptide bonds of the peptidoglycan, a major component of the bacterial cell wall.
In the rabbit sera immunized with this enzyme, there were detected neutralizing and precipitating antibodies which reacted with homologous antigen as well as commercial protease originated from Aspergillus oryzae. However, the antibodies made no response with purified trypsin, reflecting the immunological difference between trypsin and above mentioned fungal proteases.

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A septum-rich fraction was prepared from Triehophyton mentagrophytes, and the ultrastructural investigation of septa was carried out by shadowing and thin section electron microscopy before and after enzymatic and/or alkaline treatments. Tri-lamellar structure, consisting of an electron lucid middle layer and two outer electron dense layers, was observed by thin section electron microscopy. By shadowing electron microscopy, the surface ultrastructure of septa exhibited a randomly orientated microfibrillar network structure, which may correspond to the electron dense outer layers of the septum observed in thin section preparations. However, shadowed septa after papain digestion revealed a spiral arrangement of microfibrils consisting of rodlet-like units, which disappeared during chitinase treatment. This spiral structure may therefore be composed primarily of chitin. It was suggested that this spirally arranged microfibrils may correspond to the electron lucid middle layer observed in thin section preparations.

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ここ数年乳児寄生菌性紅斑の急激な増加が指摘されている. 著者は昭和46年7月より昭和50年9月までに経験した本症578例について種々の検索を施行し2, 3の興味ある所見をえた.
当教室外来では昭和46年より本症の急激な増加が始まり, その最高は昭和48年の161例であつた. 陰股部では典型的間擦疹の病像を示すことが多いが, 胸部・背部では非典型的病像を認めた. 組織学的には非特異性の急性炎症像を示す. 菌要素は24例中16例は角層のみに存在したが, 8例では毛包内や一部が顆粒層に侵入している所見をえた. 32例において病巣周辺の一見病変を認めない部位での菌の検索を施行し, 16例において直接鏡検, 培養とも陽性であつた.

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