A previous paper describes the antimicorobial activity of griseofulvin against various pathogenic microorganisms, especially against dermatophytes, combined action with other antifungal agents, and drug resistance, all in vitro.
This note reports the antifungal activity in vivo and hemolytic action of this drug, Spores of Trichoplhyton mentagrophytes was inoculated to depilated skin on the back of guinea pigs (5 animals in each group) and suspension in a 1% of carboxy-methylcellulose saline solution of griscoflvin was applied orally once daily for 10 day, 5 days after the inoculation. In order to ascertain the minimal effective dose, in various doses of each group of animals was given daily 40, 20, 10, 5 mg per Kg of body weight. Animals were observed as to the redness and scaling at inoculated local sites.
The groups of 40 mg/Kg and 20 mg/Kg showed slight symptoms as early as the third day after the start of treatment and 8-10 days later the lesions were almost or completely cured. But, it was to be noted that, in a few of them even after 10 days the local sites proved still culturally positive for T. mentagrophytes in the group of 10mg/Kg, a slight improvement was noted on the fourth to fifth day and on the tenth day the lesions were almost cured, the grade of improvement being not so conspicuous as in the former groups. In the group of 5 mg/Kg, no improvment was noted during the treatment.
Topical administration was tested with a 0.5% and 1 % of griseofulvin ointments (hydrophilic pjastibase was used) on experimental trichophytosis. A 1 % griseofulvin ointment proved effective. cure rate being about 75%, whereas a 0.5% ointhment showed no effect.
In an attempt to know the mode of action of griseofulvin on trichophyton, the following test was done : the infected scals of guinea pigs enclosed in a capsule of stainless steel wire, was put in a 0.5 % griseofulvin solution, then the material was picked out at definite intervals and tested for the growth of the fungus on Sabouraud's dextrose agar. This experiment showed that griseofulvin was not fungicidal even at higher concentration, because the fungus grew well after the long lasting contact (24 hours). However, it was to be noted that the obtained from a 0.5% of griseofulvin solution after 24 hours showed marked morphological changes both macroscopically and microscopically.
Hemolytic action of griseofulvin on human and rabbit erythrocytes were tested in vitro. Complete hemolysis was observed as to both erythrocytes in 200 mcg/ml or more of the drug solution, but not in 100 mcg/ml or less, whereas other antifungal agents (i.e., nystatin) showed more toxic effects.

抄録全体を表示

Effects of autonomic suppressants on experimental mycoses in mice were studied.
Intranasal inoculation of fresh cells of Candida alhicans caused a marked lung consolidation in mice. The death as well as lung consolidation could be prevented by prior injection of an adequate dose of autonomic suppressants. These results may show that the production of lung hemorrhage may be due to the powerful vasodilator effect of Candida albicans cells, probably acting on nerve endings. It was also confirmed that when Candida albicans, Aspergillus fumigatus or Cryptococcus neoformans wereinoculated intra-nasally, intraperitoneally or per os into mice, earlier disappearance of the inoculated fungi in thorax, lung, digestive tube, liver, spleen and kidney was seen in the chlorpromazine treated mice than in the non-treated.

抄録全体を表示

Tsuchiya et al, have been reporting the identification methods of Candida and other yeasts by means of serologic tests. In an attempt to confirm the usefulness, we asked them the serologic identification of the strains, identified morphologically and biologically in the clinic to which the auther belonged, without telling them my results obtained.
It was shown that of 106 test strains, 101 strains (97.1%) were identical with two exceptions which developed spontaneous agglutination. Thus it may be concluded that the serologic test is reliable in identification of Candida and other yeats.

抄録全体を表示