Morisita et al (1963) found an enzyme, in the body fluid of Ascaris lumbricoides, which dissolved Trichophyton and Aspergillus. Furuhashi (1964) reported a lytic action of the body fluid on Penicillium, Streptomyces and Mycobacterium. Further investigations with Morisita et al (1965) indicated the fungi-lytic action of the body fluid had depended on lipoprotein lipase.
On the other hand, no lipoprotein lipase had ever been reported in invertebrates, up to the present. Yamada (1965) isolated an enzyme from the pancreas extracts of pig, dog and rabbit, which showed fungi-lytic action and identified this enzyme as the adrenalin sensitive lipase of microsome fraction of pancreas cells. This is probably the first report on the identification of adrenalin sensitive lipase in pancreas cells. Because, no fungi-lytic action was shown in lipase isolated from pancreas.
In the author's investigations, lytic activities were observed in the F-fraction containing adrenalin sensitive lipase extracted from pig pancreas, on the all of Penicillium, Aspergillus, Trichophyton, Streptomyces and Mycobacterium. Period, however, requested for the lysis of cells after the contact of enzymic solution with cell walls, differed from 15 minutes to 6 hours, respectively, in individual fungal or bacterial cells. Optical-microscopic and electron-microscopic observations on the morphological changes in cells, according to the lytic action of adrenalin sensitive lipase, were as follows; changes in the sensitivity of hyphae in staining, ghost phenomenon, swelling and rupture of cell wall, and finally the flow out of cell protoplasms.

抄録全体を表示

わが国においては, これまで, 動物のringwormに関する研究報告は極めて少ない.特に犬のringwormについては, 先に著者が発表した犬5例のMicrosporum canis感染例が報告されているのみである.
拷者は先般から東大附属家畜病院の患畜についてringwormの検索を行なって来たが, 今回東京都内の犬2例および東大薬学部の実験動物であるラッテ32例のring-wormから, 両者共に, Trichophyton mentagrophytesを検出することが出来た.
これらの動物のringworm病変部から分離した菌株はいずれもT.mentagrophytesのgranular typeのものであり, これを土壌培養したところ, 本菌の不完全なcleistotheciaが形成された.この不完全cleistotheciaには子嚢の代りに多数のmicroconidia類似の胞子が内蔵されていた.また周辺部菌糸の形態はArthroderma属のそれと類似していた.
本報告においては犬およびラッテのringwormについての臨床所見ならびに原因菌であるT.mentagrophytesについての真菌学的所見について記載した.

抄録全体を表示

The patient was a 33-year-old man. He had had tinea capitis and tinea glabrosa from his childhood. In addition, finger tip-to hen egg-sized masses developed on the occipital and nuchal regions. On incision they discharged abundant pus, and fistulas, difficult to heal, were left. Fistulas were also developed spontaneously. With years, many subcutaneous abscesses and fistulas appeared one after another on the back; neck and chest. Some lesions showed fluctuation on palpation. Pustules, ulcers and scars were seen between the above lesions. The covering of the pustules was thin and could be broken easily. The fistulas were 0.5 to 1.0 cm deep and some extended burrow-ing horizontally by 3-4 cm. Ulcers were shallow and not beyond coin size. Their floor was uneven. A thumb tip sized hemispherical tumor was present on the fore-chest and a similar tumor of hen egg size was seen on the side of the chest. They fluctuated on plapation and were covered with normal skin. Puncture of the tumors re-vealed much purulent material, which showed the lesions to be deep-seated abscesses. They were not tender on pressure. Spontaneous pain was not complained of either. Erythemato-squamous plaques, nail to palm sized, were seen on the trunk and the extremities. Nails of the right thumb and the right IV finger were discolored and deformed. A few lymphnodes were swollen to thumb tip size in the occipital region and axillas. A lymphnode in the left thigh was also swollen to hen-egg size. The above lymphnodes were shown to contain abundant pus on incision.
In the KOH preparations of the scales of plaque-like lesions, the pus and the nail, numerous mycelium threads were detected. Histologic examination revealed numerous mycelia in the dermis, the subcutaneous tissue and the affected lymphnodes on PAS staining. No colony grew on Sabouraud's glucose agar inoculated with pus or specimen from the deeper suppurative lesion. Brain heart infusion glucose blood agar was used successfully to culture colonies of the suspected fungus. The isolated fungus was identified as T. ferrugineum, though T. verrucosum was also considered. The patient had been treated unsuccessfully with antibiotics, antiphthisica, adrenocortical hormones, potassium iodide and griseofulvin. After amphotericin B was administrated by intravenous dripping for 40 days (700 mg in total), the lesions were remarkably improved.

抄録全体を表示