Fluorochrome staining with chromomycin A
3 (CMA) was used to characterize and compare the CMA banding patterns of chromosomes in 14 accessions of 12 species of major
Citrus species. All accessions had 2n = 18 chromosomes. These chromosomes were classified into seven types based on the number and position of CMA-positive bands: A: two telomeric and one proximal band, B: one telomeric and one proximal band, C: two telomeric bands, D: one telomeric band, E: without bands, F: one proximal band, and Dst: type D with a satellite chromosome. Each accession possessed two to six types of chromosomes and unique CMA banding patterns. The CMA banding patterns were 2B + 8D + 8E in
C. medica, 1B + 1C + 8D + 8E in
C. limon, 2B + 9D + 7E in
C. aurantifolia, 1A + 1B + 1C + 7D + 8E in
C. aurantium, 2B + 2C + 7D + 7E in
C. sinensis, 3A + 3C + 4D + 8E in
C. maxima, 2A + 3C + 6D + 7E in
C. paradisi, 2B + 2C + 12D + 2E in
C. ichangensis, 2A + 5C + 8D + 3E in
C. latipes, 1B + 11D + 4E + 2Dst in
C. micrantha, 2B + 1C + 11D + 3E + 1F in
C. macroptera, and 3B + 1C + 8D + 3E + 2F + 1Dst in
C. hystrix.
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