The Japanese Journal of Veterinary Science
Online ISSN : 1881-1442
Print ISSN : 0021-5295
ISSN-L : 0021-5295
Volume 41, Issue 2
Displaying 1-10 of 10 articles from this issue
  • Masamitsu KANOE, Mitsuyoshi TODA
    1979 Volume 41 Issue 2 Pages 97-102
    Published: April 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    In order to detect bovine serum antibody against Fusobacterium necrophorum, serological investigation was made on bovine isolates of this species by the agar gel double diffusion test. Precipitin lines were observed between most of the HC1-heat extracts prepared from these strains and four anti-F. necrophorum sera. Precipitin was developed in rabbits inoculated experimentally with the organism, in the late stage of infection. Precipitin lines appeared between concentrated antigen prepared from the VPI 2891 strain and 16 of 23 sera from cattle affected with hepatic abscess, and between this antigen and 4 of 88 sera from healthy cattle.
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  • Katsuya ISHIHARA, Misao ONUMA, Tsuyoshi OHTANI
    1979 Volume 41 Issue 2 Pages 103-108
    Published: April 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    A normal peripheral lymphocyte count was obtained on the basis of age in a herd of 185 healthy cows of the indigenous Japanese Black breed screened by physical checkup, blood examinations, serum biochemical tests, and bovine leukemia virus-serum antibody test. With normal values and the Bendixen index as references, diagnostic criteria were determined for preleukemic Japanese Black cattle. In them, the lymphocyte count was judged from three rounds of tests performed at intervals of at least three months. Examined cattle were classified into negative (Group 1, 83.4%), suspicious (Group 2, 13.0%), and persistent lymphocytosis (Group 3, 3.6%) groups. The results obtained indicated that the positive rate was lower than in one test of a grouping (expressed in roman numerals). However, evaluation of persistent lymphocytosis was more reliable. Within the same year in which the lymphocyte counts were obtained, two of the seven cattle diagnosed in Group 3, and one of the twenty-tive diagnosed in Group 2 two years later suffered from leukemia. The cattle were assigned to one of the three groupings by two or three testings in each round. Only one case was classified as Group III every time. Rather than serving simply as a part of early preventive measures to remove preleukemic cattle from the herd, this lymphocyte count test is considered to be a significant index of pathological conditions in the leukemic state and a forewarning of its onset.
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  • Kanji MATSUI, Hiroshi SAWAZAKI
    1979 Volume 41 Issue 2 Pages 109-117
    Published: April 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    The QRS complex in A-B lead of an animal varied specifically in wave form from one developing stage of the animal to another. In the caprine fetus and chick embryo they showed completely the same wave form as in the respective adult, the rS type, in the second half of fetal life. In the dog, however, they varied in a different manner, exhibiting the same wave form as those in the adult dog, the Rs type, in the early neonatal stage. The present paper deals with the relationships among the alteration in the wave form of the QRS complexes, morphological changes in the heart, and the formation of specific cardiac muscle in the ventricles in the fetal and neonatal stages. It was presumed that the alteration in the wave form of the QRS complexes might have been caused by quantitative changes in the relation to the ratio of right to left ventricular mass in neonatal dogs, and by qualitative changes in the penetration of Purkinje fibers into the free ventricular walls in chick embryos and caprine fetuses. The most marked difference in the altering process in the wave form of QRS complex between the two groups observed might be related to the penetrating stage of Purkinje fibers into the outer layer of the free ventricular wall.
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  • Kinji SHIROTA, Reiji TAKAHASHI, Kosaku FUJIWARA, Atsuhiko HASEGAWA
    1979 Volume 41 Issue 2 Pages 119-127
    Published: April 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    Interstitial nephritis was observed in 118 of 215 (55%) dogs autopsied during a period from 1974 to 1975. More than 59% of dogs aged 4 years or over were found to have the renal lesions, while only 10% of these less than 1 year of age. Either diffuse or focal infiltration of mononuclear cells with fibrosis was recognized in the interstitium of these cases, some of which have very extensive tubular degeneration and necrosis. In 92 of these cases (78%) having interstitial nephritis there were diffuse or focal glomerular lesions characterized by thickening of the capillary wall, mesangial proliferation, hyalinization, sclerosing and amyloid deposition. Filariasis was the most frequent complication being detected in 67% of dogs with interstitial nephritis. On the other hand, interstitial nephritis was seen in 79 out of 102 (78%) dogs with filaria, while in 39 of 113 (35%) dogs without filaria. No inclusions suggesting canine hepatitis virus infection were seen in all cases examined. Another group of 26 dogs with interstitial nephritis were examined for leptospiral infection, revealing no leptospirae in the kidney tissue and only one antibody positive case.
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  • Katsumoto KAGOTA, Toshio IWASE, Toshiyuki KOJIMA, Masayoshi NIIYAMA, S ...
    1979 Volume 41 Issue 2 Pages 131-138
    Published: April 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    This feeding experiment with young pigs was conducted for 28 days to evaluate the growth effect of dietary diammonium citrate (DAC) by using three diets in which essential amino acids were supplied at the minimal requirement level for swine recommended by N.R.C. [12]. The three diets were: 1) diet restricted non-essential amino acids (basal diet); 2) basal diet plus 3.75% DAC (DAC diet); and 3) diet adjusted isonitrogenously to the DAC diet by natural feedstuffs (positive control diet). The average daily gain in the pigs given the DAC diet was greater than that in those fed the basal diet (P<0.05) and in those administered the positive control diet (P<0.10). The results indicate that the supplimentation of DAC can improve growth when non-essential amino acids are restricted in the basal diet under conditions of adequate essential amino acids and energy supply.
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  • Kazumasa SHIMIZU, Yukio KUROSU, Shinjiro NAKAJYO, Norimoto URAKAWA
    1979 Volume 41 Issue 2 Pages 139-149
    Published: April 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    Ouabain was examined for effect on the mechanical response of the ileum isolated from eleven animal species, dog, cat, rabbit, guinea pig, rat, vole, mouse, cock, pigeon, frog and toad. In isotonic recording, ouabain induced a sustained contraction in ileum isolated from some species. It caused a transient contraction on following by a relaxation in others. The ouabain sensitivities of a contractile response in the ilea from eleven species, as demonstrated by the estimation of ED50, was divided into four groups. That is, a high sensitive group; frog (ED50=9.0×10-8M), cat (9.3×10-8M), pigeon (3.5×10-7M) and cock (5.1×10-7M), a sensitive group; rabbit (1.1×10-6M), vole (2.3×10-6M) and guinea pig (2.7×10-6M), a low sensitive group; mouse (1.7×10-5M) and rat (9.7×10-5M) and an insensitive group; toad (>10-3M). Although ED50 in the dog was not able to be calculated, its sensitivity seems to belong to the high sensitive group. On the other hand, ED50 of acetylcholine (Ach) in thc ilea, isolated from these species were estimated in a narrow range of concentration from 5×10-9M to 5×10-8M. There were species differences in ouabain sensitivity for ileal contractile response but not in Ach sensitivity. A distinct correlation was found between the mechanical response to ouabain and the inhibitory potency of ouabain for Na, K-ATPase reported by Repke et al. These results seem to confirm that ouabain may induce a contraction by a mechanism which inhibits an electrogenic Na pump or Na, K-ATPase in the intestinal smooth muscle cell and which induces a depolarization.
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  • Masamichi KUROHMARU, Takao NISHIDA, Koshi MOCHIZUKI
    1979 Volume 41 Issue 2 Pages 151-165
    Published: April 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    Morphological changes of developing duodenal villi in prenatal, postnatal and adult hamsters were examined in light and electron microscopy. Scanning electron microscopic observation revealed that the duodenal villi first appeared at 13.5 days of gestation as square-like protuberances. At birth, the shape of villi changed from square to columnar. After suckling period, the shape abruptly changed into flat leaf-like, and then completed the morphological differentiation into adult duodenal villi. On the other hand, growth and differentiation of surface structures such as large blebs at the apices of villi and creases on the lateral surface gradually proceeded with age. Light and transmission electron microscopic observation exhibited that at the time of first appearance of villi, the surface epithelium turned from stratified to single layered. Single-layered epithelial cells developed rapidly to accomplish their growth to a great degree about one day before birth. At birth, each of these absorptive epithelial cells had a structure almost similar to that in adult.
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  • Yuji FUJIKAWA, Kazue SAIJO, Fujio OBATA, Noboru FUJIE
    1979 Volume 41 Issue 2 Pages 167-176
    Published: April 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    Field trials were carried out in 20, 425 birds on 10 farms to evaluate a lyophilized vaccine prepared from herpesvirus of turkey (HVT) in prophylactic efficacy against Marek's disease (MD), in comparison with cell-associated HVT vaccine. There was no significant difference (P>0.1) in protection rate between both vaccines. So the lyophilized vaccine was proved to be equivalent to the cell-associated in the efficacy. The chickens of one farm vaccinated with the lyophilized vaccine and those of another farm with the cell-associated vaccine were examined periodically for antibody by the agar-gel precipitin test (AGP). Antibody response due to vaccination could be detected on both farms when homologous (HVT) antigen was used, but not when heterologous (MDV) antigen was used. It was suggested that AGP might be useful for surveillance of field flocks after vaccination. Two groups of chickens with and without maternal antibody, respectively, were inoculated with the lyophilized vaccine and examined for appearance and persistence of antibody by both AGP and indirect immunofluorescent antibody technique (IFA). No significant differences were found (P>0.05) between both groups in the rate of chickens positive for AGP antibody after vaccination. A quantitative study by IFA, however, indicated that antibody production was delayed to a great extent in the group with a maternal antibody titer of 320 and higher, even if antibodies were derived from MDV-infection. This delay was overtaken by the 7th week postvaccination.
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  • Toshiaki KUSHIDA
    1979 Volume 41 Issue 2 Pages 177-179
    Published: April 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
  • Gihei SATO, Masayoshi ASAGI, Hiroshi YAMAMOTO
    1979 Volume 41 Issue 2 Pages 181-183
    Published: April 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
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