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Xuenan XUAN, Taisuke HORIMOTO, Joselito A. LIMCUMPAO, Ken NEMOTO, Yuki ...
1990 Volume 52 Issue 5 Pages
899-905
Published: October 15, 1990
Released on J-STAGE: February 14, 2008
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Monoclonal antibodies (MoAbs) were used to identify the hemagglutinin of canine herpesvirus (CHV). The inhibition of viral hemagglutination (HA) activity was observed with MoAbs against 41 kD glycoprotein, while no hemagglutination-inhibition (HI) activity was observed with those against 145/112 kD and 80 kD glycoproteins, suggesting that the 41 kD glycoprotein is the hemagglutinin of plaque-selected virus of CHV YP11 strain used as immunogen for MoAb production. All of the HI MoAbs also showed HI activities against HA antigens which were prepared from cells infected with other CHV strains, namely, F-205 V and Glasgow CHV2 reference strains, eight Japanese isolates, and the original YP11 strain. However, on immunoblotting analysis, a 47 kD protein band was detected in these strains by the HI MoAbs. These data suggest that the 47 kD glycoprotein is the common molecule of the hemagglutinin among CHV strains and the plaque-selected virus of YP11 strain appears to be a mutant whose molecular weight of the hemagglutinin changed into 41 kD.
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Naoyoshi SUZUKI, Haruhisa SAKURAI, Atsushi SAITO, Ikuo IGARASHI, Yoshi ...
1990 Volume 52 Issue 5 Pages
907-914
Published: October 15, 1990
Released on J-STAGE: February 14, 2008
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Tachyzoites of Toxoplasma gondii were killed in mouse macrophage and human somatic cell monolayers by a novel synthetic peptide (Obiopeptide-1) which is a Glycil-penta-Glutaminate (GpG) derivative of native Obioactin. In view of the worldwide prevalance of this protozoan disease and the lack of effective treatments, Obiopeptide-1 may be a new and unique antimicrobial active substance of non-antibiotic chemotherapeutic agents for intracellular parasites, T. gondii and associated nonspecific hypoimmune responses that occur in infected hosts.
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Eiichi KAWAMOTO, Takuo SAWADA, Tsutomu MARUYAMA
1990 Volume 52 Issue 5 Pages
915-921
Published: October 15, 1990
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Prevalence and some properties of Pasteurella multocida in rabbits kept at laboratory animal facilities and commercial rabbitries, and in their environment were investigated. A total of 1, 147 nasal swab samples from 1, 147 rabbits and 126 samples from their environment were subjected to the isolation of P. multocida. The bacteria were isolated from 199 (29.8%) of 668 rabbits in laboratory animal facilities and from 1 (0.2%) of 479 rabbits in the rabbitries. Isolation rate of P. multocida was low (0.9%) or high (44.9%) in the facilities with or without the monitoring for the presence of the bacteria, respectively. The highest rate of the isolation from rabbits was recorded at 10 to 12 months of their housing time. Thirty-nine cultures (31.0%) of air and the surfaces of floors, tips of water bottles, and cages were positive for P. multocida and isolation rate of the bacteria was high (78.6%) in the air. Biological and biochemical properties of the isolates were identical except for indole production and raffinose fermentation. The isolates were susceptible to antibiotics tested except for clindamycin, serologically similar in the gel-diffusion precipitin test and weakly virulent for mice. The present results suggested that these P. multocida isolates were the causal agent of rabbits rhinitis (snuffles) in Japan.
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Emiko ISOGAI, Hiroshi ISOGAI, Nobuhiro FUJII, Keiji OGUMA
1990 Volume 52 Issue 5 Pages
923-930
Published: October 15, 1990
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Leptospiral lipopolysaccharides (LPSs) extracted from Leptospira interrogans serovars copenhageni and hebdomadis were tested for the biological effect to mouse B, T and NK cells. Each leptospiral LPS was a potent mitogen for spleen B cells. Activation of the cells was also expressed by polyclonal B cell activation. In contrast, mitogenicity for T cells, induction of interleukin-2 (IL-2) secretion in T cells and increase of tumor-killing activity and chemiluminescence in NK cells were not observed after stimulation with leptospiral LPS. After intravenous injection of leptospiral LPS in mice, the spleen and lymphnodes were examined by histocytochemical technique. Increase of Ig-bearing lymphocytes was recognized while decrease of T cells was observed in the lymphoid organs. Mitogenic response to PHA, Con A and PWM decreased with relation to the T cell depletion. In conclusion, it is apparent that leptospiral LPS possess marked immunological potencies on B cells but not T and NK cells. The biological effects of leptospiral LPS were common ones as LPS but the level was considered to be different from classical LPS such as Escherichea coli LPS
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Hideki TATE, Hiroshi KODAMA, Hisao IZAWA
1990 Volume 52 Issue 5 Pages
931-937
Published: October 15, 1990
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The infectivity of infectious pancreatic necrosis virus (IPNV) for rainbow trout (Oncorhynchus mykiss) mononuclear leukocyte subpopulations was investigated to determine the mechanisms of immunosuppression caused by the virus. IPNV was recovered from nylon wool-adherent, surface immunoglobulin (Ig)-positive leukocytes of head kidney, spleen and peripheral blood collected from virus-inoculated fish with higher titers than non-adherent, Ig-negative cells. Non-adherent cell population showed mitogenic response to phytohemagglutinin and concanavalin A but not to lipopolysaccharide. Conversely, the responses of adherent cells to these mitogens were weak. Mitogenic response and non-specific cytotoxicity of head kidney leukocytes significantly decreased by the inoculation of fish with the virus. These results suggest that the suppression of immune responses is involved in the establishment of carrier state in fish after infection with IPNV.
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Shunji SUGII, Yoshikazu HIROTA
1990 Volume 52 Issue 5 Pages
939-945
Published: October 15, 1990
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Calcium-dependent and -independent bovine immunoglobulins (IgM and IgG) reactive with melibiose were isolated by affinity chromatography on melibiose-coupled Sepharose 4B. Hemagglutination and hemagglutination inhibition were carried out to study their carbohydrate specificities. Human and animal erythrocytes were agglutinated by these bovine IgM, whereas those were not by bovine IgG at the highest concentrations used. Neuraminidase- and pronase-treated erythrocytes were more strongly agglutinated by these IgM than untreated ones. On the other hand, melibiose-reactive human immunoglobulins isolated from AB serum showed strong hemagglutinating activities to rabbit erythrocytes. Hemagglutination of neuraminidase-treated human type B erythrocytes by calcium-independent bovine IgM reactive with melibiose was effectively inhibited by galactose, methyl α-D-galactopyranoside and melibiose, whereas that was not by methyl β-D-galactopyranoside, lactose, and other substances at the highest concentrations used. Similar results were also obtained in hemagglutination inhibition with untreated rabbit erythrocytes and calcium-independent human immunoglobulins (IgM and IgG) reactive with melibiose. However, hemagglutination of pronase-treated human type A erythrocytes by calcium-dependent bovine IgM reactive with melibiose was not at all inhibited by these substances at the highest concentrations used. From these results, bovine serum is found to also contain antibodies with a specificity for α-linked galactosyl residues as found for human AB serum reported previously.
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Jun-ichi SUZUKI, Norio KATOH
1990 Volume 52 Issue 5 Pages
947-954
Published: October 15, 1990
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Cysteine protease was found to be present in bovine milk that catalyzed casein as the substrate. The protease was activated by reducing agents such as 2-mercaptoethanol and inhibited by monoiodoacetic acid, but not affected by the addition of phenylmethylsulfonyl fluoride, calcium or ethylene glycol bis (β-aminoethyl)-N, N, N', N'-tetraacetic acid. The protease activity was linear as a function of protein amount and incubation time, and showed maximum at pH 6.0. By Sephacryl S-200 chromatography, at least two types of cysteine proteases having molecular weights of 45kDa and more than 150kDa were detected. The activity was increased in mastitic milk, and well correlated with the stages of mastitis, as indicated by the California mastitis test, somatic cell count and protein concentration. These results suggested that cysteine protease (s) is involved in the pathogenesis of mastitis.
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Yukinobu TOHYA, Makoto TSUBAKIMOTO, Taisuke HORIMOTO, Eiji TAKAHASHI, ...
1990 Volume 52 Issue 5 Pages
955-961
Published: October 15, 1990
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In the culture fluid from cells infected with feline calicivirus (FCV) F4 strain, the infectious and smaller non-infectious subunit particles were detected by complement fixation (CF) test after sucrose gradient centrifugation. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analyses confirmed the existence of the subunit particles of FCV, and showed that the infectious and subunit particles were mainly composed of 65K capsid protein. The subunit particles were further purified by ion-exchange chromatography and sucrose gradient centrifugation. The purified subunit and infectious particles had the same neutralizing epitope on 65K protein detected by immunoblot analysis with a neutralizing monoclonal antibody. Antigenic comparison between the infectious and subunit particles by the CF tests using an antiserum against heterologous strain of FCV F14 indicated that the subunit particles might have more highly conserved antigens of FCV than the infectious particles.
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Akira SHIMIZU, Junichi KAWANO, Tetsuya FUJINAMI, Junichiro OZAKI, Shig ...
1990 Volume 52 Issue 5 Pages
963-968
Published: October 15, 1990
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Staphylococci were found in the tonsils of 121 (75.2%) of 161 cattle. There were 15 different species, 10 belonging to novobiocin-sensitive species. The most predominant species was S. simulans (79.3% of the 121 carriers), followed by S. aureus (20.7%), S. chromogenes (10.7%) and S. epidermidis (8.3%). The other 11 species were present in 0.8 to 5.8%. Twenty-six unidentifiable isolates were isolated from 26 (21.5%) carriers. Sixty-two (51.2%) of the 121 carriers yielded two to five Staphylococcus species together while only one species could be found in each of the other 59 (48.8%). Combinations of S. simulans and other species were most frequently encountered in 50 (41.3%) of the 121 carriers. Twenty-four (96.0%) out of 25 S. aureus isolates, 3 (42.9%) of 7 S. hyicus isolates and 45 (25.4%) of 177 coagulase-negative staphylococci (13 species and unidentifiable isolates) isolates were phage typable. Most of S. aureus isolates were lysed by bovine phages 119 (n=16) or 116 (n=5). Thirty-three (25.4%) of 45 coagulase-negative staphylococci typable isolates with Pulverer's phage set showed the phage pattern ph5/ph9/ph10/ph12/ph13/U4/U14/U16/U20/U46. The tonsils of cattle thus appear to be a suitable environment for Staphylococcus species, particularly novobiocin-sensitive species.
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Ikuo IGARASHI, Ryusuke HONDA, Teruo SHIMADA, Kazuro MIYAHARA, Haruhisa ...
1990 Volume 52 Issue 5 Pages
969-977
Published: October 15, 1990
Released on J-STAGE: February 14, 2008
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When 8-week-old BALB/c mice were sensitized with two intramuscular injections of Toxoplasma lysate antigen (TLA) at 2 week interval, the numbers of sIg(+), Thy-1, 2(+), Lyt-1, 2(+) Lyt-2, 2(+), and Asialo GM1 (ASGM1) (+) cells in the spleen, liver and peripheral blood increased by 2 to 4 times over those found in unsensitized mice of the same age. When TLA-sensitized and unsensitized mice were infected with Babesia, 4 of 10 (40%) of the TLA-sensitized mice survived infection, while none of the unsensitized control mice lived longer than 14 days after Babesia infection. By contrast, sensitization of nude mice with TLA had no effect on survival, and mice did not live more than 12 days. The number of thymic Thy-1, 2(+) cells decreased in TLA-sensitized and unsensitized BALB/c mice by almost 80% within 10 days after infection (AI). During the same time, the numbers of B cells, T cells, and NK cells increased in the spleen, liver and peripheral blood of both sensitized and unsensitized mice. Especially notable were increases in numbers of Lyt-2, 2(+) cells in the spleen and blood and increases in numbers of NK cells in the spleen, liver and blood in both TLA-sensitized and unsensitized mice. When spleen cells from TLA-sensitized and unsensitized mice were cultured in the presence or absence of TLA for 6 days, assays for cytotoxicity using NK-insensitive P-815 target cells and NK-sensitive YAC-1 target cells demonstrated higher rates of cytotoxicity in cultures of TLA-sensitized spleen cells.
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Koichi NOMURA, Kohtaro KAWASOE, Yasuaki SHIMADA
1990 Volume 52 Issue 5 Pages
979-983
Published: October 15, 1990
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Of eight mongrel bitches, the antimesometrial side of the nonpregnant left horn of the uterus at the pregnant or nonpregnant luteal phase was scratched with a Kirschner's wire. Cystic endometrial hyperplasia (CEH) was induced in seven of the eight bitches (87.5%). No difference in the incidence of CEH in the left horn was seen between the pregnant and the nonpregnant groups. Histological examinations showed CEH with a dilatation of the basal glands, resembling "Swiss cheese endometrium".
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Motohiro HORIUCHI, Hiroshi KODAMA, Takeshi MIKAMI
1990 Volume 52 Issue 5 Pages
985-994
Published: October 15, 1990
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For the identification of serotype-specific antigens of Marek's disease virus (MDV) serotype 1 (MDV1) or serotype 2 (MDV2), a total of 24 hybridoma clones, secreting monoclonal antibodies (MAbs) against CVI-988 (MDV1) or HPRS-24 (MDV2) strain, were established and characterized by immunofluorescence assay, virus neutralization and immunoprecipitation analysis. Based upon the molecular weights (mol. wt.) of the immunoprecipitated polypeptides, the MAbs were subdivided into 7 groups. Among them, two groups of MAbs reacted with antigens that have not been reported, were identified. MAbs belonging to the first group reacted with CVI-988- and MDV2-specific antigens with mol. wt. ranging from 29K to 34K (29/34K). This antigen was not found in cells infected with Md/5 and JM strains of MDV1, and the results of kinetic analysis of antigen expression showed this antigen appeared to be related to late membrane antigens. MAbs belonging to the second group immunoprecipitated MDV2-specific antigens with mol. wt. of 37K, 33K and 31K from HPRS-24-infected cells or with those of 37K, 34K and 31K from SB-1 (MDV2)-infected cells, and these antigens appeared to be related to early antigens. MAbs belonging to the other 5 groups included those which recognized similar antigens reported previously or the antigens characterized insufficiently in this study.
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Hideto KANEKO, Mineo HAYASAKI, Isamu OHISHI
1990 Volume 52 Issue 5 Pages
995-1000
Published: October 15, 1990
Released on J-STAGE: February 14, 2008
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Excretory-secretory (ES) products collected from adult Dirofilaria immitis cultured in vitro were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. ES products of male (M-ES) and female (F-ES) worms were separated into 16 and 21 bands by SDS-PAGE with Coomassie blue and silver staining, respectively. The antigenic bands were then analyzed by immunoblotting, using pooled sera from dogs that had naturally contracted D. immitis. Sera from dogs with microfilaremic infection showed 7 bands in M-ES and 10 bands in F-ES, while those from dogs with occult infection revealed 3 bands in M-ES and 10 bands in F-ES. Among these bands, those of 14, 18, 21, 22, 29, and 32 kilodaltons (Kd) were common to M-ES and F-ES, those of 39 and 44Kd were specific to M-ES, and those of 20, 38, 43, 53, 63, 90, 110, 125 and 136Kd were specific to F-ES.
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Takashi HASEGAWA, Kiyomi MATSUOKA, Kenichiro ONO, Toshihiro WATARI, Ry ...
1990 Volume 52 Issue 5 Pages
1001-1006
Published: October 15, 1990
Released on J-STAGE: February 14, 2008
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A canine case of Coombs' test positive and antinuclear antibody-negative hemolytic anemia was examined because of the development of skin lesions after 18 months treatment with prednisolone. Histopathological examination of biopsy specimens obtained from skin and oral mucosa revealed the acantholysis, edematous lesions of the stratum basale and mononuclear cell accumulation in the dermis. Deposits of immunoglobulin G and complement factor 3 were detected at the intercellular and dermoepidermal junction by the direct immunofluorescent test. From these results, the case was considered to be an autoimmune disease caused by distinct antibodies against different organs.
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Ann GRAIL, David A. HARBOUR
1990 Volume 52 Issue 5 Pages
1007-1013
Published: October 15, 1990
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DNAs prepared from whole feline embryo cells infected with a standard laboratory strain of FHV-1, B927, and 50 wild type isolates were digested with a variety of restriction endonucleases. The resulting fragments were separated on agarose gels and Southern blotting performed. To visualize the fragments, B927 DNA was purified by pulsed field gel electrophoresis, labelled with α
32P and used as a hybridization probe. With most enzymes a large number of the isolates displayed altered mobilities of several fragments with some fuzzy band heterogeneity. A few isolates gave distinct cleavage patterns, in particular using the enzymes Bam HI, Cla I and Sac I. It is suggested that different strains of FHV-1 exist but that changes in the viral genome occur only sporadically and thus may not be readily detected.
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Makoto SUGIMURA, Daiji SHIROGANE, Yasuro ATOJI, Yoshitaka SUZUKI, Kazu ...
1990 Volume 52 Issue 5 Pages
1015-1021
Published: October 15, 1990
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S-100 protein-immunoreactive cells of the lymph node were examined in the duck and 9 mammalian species, such as guinea pigs, dogs, cats, horses, pigs, goats, cows, Japanese serows and crab-eating monkeys. S-100 protein was detected in follicular dendritic cells (FDC) and tingible-body macrophages (TBM), sinus and parenchymal macrophage (MP), sinus endothelial cells (SEC) and interdigitating reituclum-like cells (IRC) in the node of mammalian species, but not in the duck except nervous elements. S-100-positive FDC and TBM were detected in germinal centers of the nodes in all mammalian species, but immunoreactivity of the other 3 cell types varied according to animal species and individuals of the same species. S-100 α subunit was detected in FDC, with the exception of those of the duck and guinea pig. The subunit was also detected in SEC of the dog, cow and Japanese serow. In the guinea pig, a unique S-100 α-positive giant dendritic cell (GDC) was found in the subsinusal cortical area. In addition, S-100 immunoreactive lymphocytes were observed in the paracortex-equivalent area of pig nodes. Arterial endothelial cells of the pig and cow were immunoreactive to S-100 β subunit.
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Kazunori IKE, Katsumi KUME, Kazuyoshi KAWAHARA, Hirofumi DANBARA
1990 Volume 52 Issue 5 Pages
1023-1027
Published: October 15, 1990
Released on J-STAGE: February 14, 2008
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One hundred and fifty one Escherichia coli strains were isolated from broiler chickens with coli-septicemia in Aichi (63 strains), Shizuoka (58 strains), and Kagoshima (30 strains) prefectures from 1980 to 1987, and their O and pilus antigens were serologically typed. One hundred and twenty five strains (82.8%) were typed into 23 O serogroups, and twenty six strains (17.2%) remained untypable. The predominant O serogroups were O2 (35 strains, 23.2%) and O78 (24 strains, 15.9%). Distribution of O serogroup was different, depending on prefectures where they are isolated. In total, 109 strains (72.2%) possessed Type 1 and/or Fmsha pili (Type 1; 41 strains, Fmsha; 22 strains, and Type 1 and Fmsha; 46 strains), and 42 strains (27.2%) were non-piliated. All the strains lacked K88, K99, 987P, F41, and Att25 pili. The ratios of piliated strains to non-piliated ones were almost the same among the three prefectures. Strains possessing Type 1 pili showed variety of O antigens, but most of the strains with Fmsha pili belonged to O2 serogroup.
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Hitoshi KITAGAWA, Yoshihide SASAKI, Katsuya ISHIHARA, Yasuhito KUWAHAR ...
1990 Volume 52 Issue 5 Pages
1029-1035
Published: October 15, 1990
Released on J-STAGE: February 14, 2008
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In order to develop an artificial model of caval syndrome (dirofilarial hemoglobinuria), heartworm-like silicone tubes were inserted into the tricuspid valve orifice and right atrium of dogs. Fifteen to 25 tubes with some knots were inserted through the posterior vena cava in 6 dogs (knot-tube group), 7 to 12 tubes without knot (small-number group) through the jugular vein in another 5 dogs, or 25 to 35 tubes (large-number group) in yet another 5 dogs. The tubes remained in the right atrium, and a part of them protruded into the tricuspid valve orifice. The number of tubes at the tricuspid valve orifice was the greatest in the large-number group. After tube insertion, the signs of so-called "caval syndrome", such as systolic cardiac murmur, jugular pulse, anemia, and so on, were observed in almost all cases of the 3 groups, the signs were severest in the large-number group. Urine hemoglobin was detected in almost all cases of the knot-tube and large-number groups, and in 1 case in the small-number group. Ascites was observed in 1 case of the knot-tube group at 6 weeks, in 1 case of the small-number group at 7 days and in 3 cases of the large-number group at 7 days after insertion.
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Shunji SUGII
1990 Volume 52 Issue 5 Pages
1037-1042
Published: October 15, 1990
Released on J-STAGE: February 14, 2008
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The binding and hemagglutinating properties of cholera toxin (CT) were studied by competitive binding assays and hemagglutination inhibition. The binding of
125I-labeled CT to neuraminidase-treated human type B erythrocytes was most effectively inhibited by ganglioside GM
1 among different inhibitors used. Other mono-, di-, and polysaccharides and glycoproteins were at least 10
5 times less potent inhibitors. On the other hand, hemagglutination of neuraminidase-treated human type B erythrocytes by CT was inhibited by lactose, galactose, hog A+H, bovine salivary mucin, porcine thyroglobulin, and fetuin, whereas that was not effectively inhibited by ganglioside GM
1 at the highest concentration. These findings suggest that the predominant binding substance for CT on human type B erythrocytes is ganglioside GM
1 and that hemagglutination requires some additonal process since the interaction of CT with ganglioside GM
1 is somehow different in hemagglutination.
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Toshifumi KOSAKA, Fusaoki KOIDE, Masato KUWABARA, Shigeo TANAKA
1990 Volume 52 Issue 5 Pages
1043-1048
Published: October 15, 1990
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Production of active oxygen by canine peripheral blood monouclear lymphocytes (PBLs) from beagle dog was examined by luminol-dependent chemiluminescence production. The canine PBLs rapidly produced the active oxygen in parallel with the number of cells when PBLs were cocultured with canine leukemia-derived CL-1 cells as target cells. Cytolysis of the target cells and active oxygen production were inhibited linearly by the addition of benzoic acid and n-propyl gallate as hydroxyl radical scavenger. However, superoxide dismutase and tiron which are scavengers of superoxide anion did not inhibit the cytotoxicity so much at low concentrations that inhibited the induction of luminol-dependent chemiluminescence. These results suggest that hydroxyl radical production by stimulated PBLs might be playing a major role of cytotoxic action in the case of canine system.
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Chikako OHTA, Atsuo HOSHI, Hitoshi GOTO, Nobuo TSUNODA, Masaaki TAGAMI ...
1990 Volume 52 Issue 5 Pages
1049-1056
Published: October 15, 1990
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Epizootiological and virological studies were conducted on foal diarrhea occurring in 3 foal-raising locations in a light horse farm from March to July, 1987. At the first location, although 27 (75%) of 36 foals had developed diarrhea, the isolation rate of rotavirus (RV) was low (5/14 feces, 36%). Many of the foals had the disease as early as 23 days after birth. At the second and third locations, 21 (27%) of 78 foals and 41 (76%) of 54 foals were affected with diarrhea. Isolation rates of RV were 90% (20/22 feces) and 100% (26/26 feces), respectively. The diseased cases were observed throughout the short period from June to early July and on foals aged from 63 to 65 days. These findings suggested the importance of RV as a causal agent of foal diarrhea at the latter location, RV and/or other agents at the former location. All the 3 strains of RV represented from each location were identified as serotype 3 by plaque reduction neutralization test against the antiserums of serotypes 1 to 6 of RV. However, the 3 strains showed low cross-reactivity with antiserum of the serotype 3 of the equine RV.
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Yoshio KOJIMA
1990 Volume 52 Issue 5 Pages
1057-1062
Published: October 15, 1990
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The fine structure of the spindle shape body (SSB) of boar spermatid was studied using testes samples fixed by perfusion. This structure appeared on the middle piece which served as the upper border of the fibrous sheath of principal piece during the transition period between the late acrosome phase to the maturation phase of spermiogenesis. The formation of this thread-like spindle form coincided with the development of the postnuclear sheath with perinuclear ring just prior to the growth of the equatorial segment of the acrosome. Likewise, the ribs of the fibrous sheath on the principal piece were observed to have already formed but have not yet completed the mitochondrial sheath. The total size of the SSB and its consisting microtubule were measured. The functional meaning of this transitory construction may involve a threshold condition on the sperm middle piece.
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Soichi IMAI, GUI RUNG
1990 Volume 52 Issue 5 Pages
1063-1067
Published: October 15, 1990
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Rumen ciliate composition was surveyed on the Mongolian gazelle, Procapra gutturosa in Inner-Mongolia, China. As a result of survey, 4 species belonging to the genus Entodinium, E. convexum, E. dubardi, E. nanellum and E. exiguum, were detected. All the ciliates were devoid of caudal appendages. Of the species detected, E. convexum was found from all the hosts with a relatively high composition rate of 53.0-100.0%. The ciliate density ranged from 12.3 to 67.7×10
4, with an average of 24.8×10
4 per 1ml of the rumen fluid.
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Soichi IMAI, GUI RUNG
1990 Volume 52 Issue 5 Pages
1069-1075
Published: October 15, 1990
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Ciliate composition in the forestomach was examined in four Bactrian camels, Camelus bactrianus, inhabiting Mongol and Steppes, then 7 genera containing 14 species with 5 formae were identified. One of these formae was a new forma, and described as Entodinium okoppensis f. cameli n. f. Six species containing one new forma were new records in the forestomach of camels. The percentage composition of genera varied with the individuals of host and the collection sites in the stomach, but Entodinium was generally predominant. Total ciliate density per milliliter of stomach fluid was 2.1×10
5 on average.
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Minoru SUZUKI, Kiitiro SITIZYO, Takashi TAKEUCHI, Toshiyuki SAITO
1990 Volume 52 Issue 5 Pages
1077-1087
Published: October 15, 1990
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Electroencephalogram (EEG) examinations were carried out on three Japanese Black calves (1, 2 and 14 week old) affected with cerebrocortical necrosis (CCN) in order to investigate the relationship between EEG abnormalities and encephalic lesions. Some neurologic signs such as circling, astasia, convulsion and opisthotonus appeared in two of the calves (case 1 and 3). The other calf (case 2), however, showed no neurologic signs. The EEGs obtained from the two calves (case 1 and 2), in the awake state, showed constant high amplitude (5-160μV) and slow (1-4Hz) activity (HASA). One of these (case 1) had necrosis mainly in the right hemisphere and showed a markedly asymmetric sharp wave. On the other hand, in case 3, the EEG showed diffuse lowered activity, and almost flat in some leads. A decrease in fast activity observed in common with all three animals. These findings suggest that one of the characteristic EEG patterns of CCN patient may be HASA and decreased fast activity. Histopathologic diagnoses of the calves were CCN in various degrees. The degrees of abnormality of the EEGs seemed to accord with the seriousness of the lesions. Because the EEG obtained from the calf having no obvious neurologic signs showed an abnormal pattern, it was considered that EEG examinations in the early stages of disorders are valuable to diagnose CCN.
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Tadashi HIRAHARA, Hisao YASUHARA, Osamu MATSUI, Shin-ichi FUKUYAMA, Mo ...
1990 Volume 52 Issue 5 Pages
1089-1091
Published: October 15, 1990
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Katsunori OKAZAKI, Kiyotaka NAKATSU, Eiichi HONDA, Tetsuo KUMAGAI
1990 Volume 52 Issue 5 Pages
1093-1095
Published: October 15, 1990
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Michio FUJITA, Hiromitsu ORIMA, Motoko SHIMIZU, Shigekatsu MOTOYOSHI, ...
1990 Volume 52 Issue 5 Pages
1097-1098
Published: October 15, 1990
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Norio HIRAYAMA, Enuh RAHARJO JUSA, Mastur AENY ROCHMAN NOOR, Kaoru SAK ...
1990 Volume 52 Issue 5 Pages
1099-1101
Published: October 15, 1990
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Toshimitsu TANAKA, Kosei GOTOH, Ryoh'ichi SATOH, Mizuho INAZU, Takayos ...
1990 Volume 52 Issue 5 Pages
1103-1105
Published: October 15, 1990
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Munehiro OKAMOTO, Masao KAMIYA, Yuzaburo OKU, Masashi OHBAYASHI, Tetsu ...
1990 Volume 52 Issue 5 Pages
1107-1108
Published: October 15, 1990
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Kenichi SAKAMOTO
1990 Volume 52 Issue 5 Pages
1109-1111
Published: October 15, 1990
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Kiyohito TAKAHASHI, Naoji TAKAHASHI, Akiyoshi YAMAMOTO, Takashi UEMURA ...
1990 Volume 52 Issue 5 Pages
1113-1115
Published: October 15, 1990
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Yoshikazu HIROTA, Nobuo KOIZUMI, Yutaka MATSUBARA, Kenkichi IMAMURA, K ...
1990 Volume 52 Issue 5 Pages
1117-1121
Published: October 15, 1990
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Hitoshi KITAGAWA, Yoshihide SASAKI, Katsuya ISHIHARA, Yasuhito KUWAHAR ...
1990 Volume 52 Issue 5 Pages
1123-1125
Published: October 15, 1990
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Kazuko OKAZAKI, Tetsuo KUMAGAI, Eiichi HONDA, Katsunori OKAZAKI
1990 Volume 52 Issue 5 Pages
1127-1130
Published: October 15, 1990
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Misao ONUMA, Yuuko OGAWA, Yoshimi KAWAKAMI
1990 Volume 52 Issue 5 Pages
1131-1133
Published: October 15, 1990
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Cha Soo LEE, Cheong Kyu PARK, Tae Kyun SHIN, Yong Joon CHO, Jong Sik J ...
1990 Volume 52 Issue 5 Pages
1135-1137
Published: October 15, 1990
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Naomi WADA
1990 Volume 52 Issue 5 Pages
1139-1141
Published: October 15, 1990
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Miho NAKAMURA, Shigeo ITO, Akira OHGA
1990 Volume 52 Issue 5 Pages
1143-1145
Published: October 15, 1990
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