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Volume 34, Issue 3
Displaying 1-4 of 4 articles from this issue
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Teiji IIJIMA1959 Volume 34 Issue 3 Pages 77-83
Published: 1959
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSCompatibility mutants such as Hfr-i, Hfr-i' and Hfr- were isolated from Hfr (Hayes' strain) by acriflavine treatment. Hfr-i, Hfy-i' and Hfr- resemble F+, Fi and F- respectively in their mating behavior, possession or lack of transmissible agent and susceptibility to infection by F. There is no difference, between Hfr-i and F+ in their mating behavior, the role as chromosome donor in recombination and possession of transmissible agent, and there is also no difference between Hfr- and F- in their mating behavior, the role as chromosome recipient in recombination and susceptibility to infection by F within the limits of the technique empolyed.View full abstractDownload PDF (717K) -
Kazuo SAITOH1959 Volume 34 Issue 3 Pages 84-87
Published: 1959
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSThe chromosomes of the haploid group in 11 species of moths from 6 families were investigated in squash material prepared with the application of acetic dahlia. The species under study and the chromosome numbers established are listed in Table 1.View full abstractDownload PDF (286K) -
3. On the nucleus and autogamy of Bacillus megatheriumTadashi HIRANO1959 Volume 34 Issue 3 Pages 88-95
Published: 1959
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSThe author confirmed that the resting nucleus of Bacillus megatherium is spherical-shaped as in the case of the higher organisms when stained by Giemsa-staining, HCI-Giemsa staining, perchloric-Giemsa-staining and Feulgen nuclear staining.
This fact is also confirmed in vivo by phase-contrast microscope.
Judging from the results obtained by phase-contrast microscopy and HCI-Giemsa staining, it is thought that the chromosome number is two in the case of Bacillus megatherium (n=2).
Each cell has one nucleus which can be observed by phase contrast microscope or by the method of Johne's cell wall staining.
The karyogamy has been observed continually by phase contrast microscope. Before the karyogamy, the cell divides into many small ones.
Each small cell has two chromosome like bodies in it. Two adjoining cells fuse each other and two nuclei fuse, too.
The karyogamy is carried out in the state of resting nucleus. The fused nucleus changes into spherical form from cylindrical one. This phenomenon can be thought as autogamy. It takes about thirty minutes in the case of the autogamy.
This phenomenon can be thought as sexual reproductive one in the life-cycle of Bacillus megatherium.View full abstractDownload PDF (5489K) -
III. The maturation division in PMC's of TperHRF1 plants having 2n=28 chromosomesGoichi NAKAJIMA1959 Volume 34 Issue 3 Pages 96-101
Published: 1959
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESS1. In the present report, the result of cytological studies on the maturation division of PMC's of TperHRF1 plants having 2n=28 chromosomes as the number for the eu-trigeneric triple hybrid was descrived. These F1 plants were obtained in 1958.
2. At the heterotypic metaphase on meiosis of PMC's of these F1 plants 0-6 bivalents and 28-16 univalents were observed (Figs. 1-7). The frequency of the bivalents in one PMC was shown in Table 1. Almost all the bivalents consisted of two elements of equal size, but in very rare cases, heteromorphic bivalent was observed (Fig. 8). Most of the bivalents were stick-shaped loosely conjugated end to end, but some of them were ring-shaped joined closely at two ends (1077 ring-shaped in 18224 bivalents). It seems that 4 of the 6 bivalents are raised from autosyndesis of the chromosomes of AB genomes of T. persicum and the other 2 may be raised from autosyndesis of which V and R genomes of H. villosa and S. cereale respectively, considering the results of cytological researches on the intergeneric hybrids between Triticum, Secale and Haynaldia and on the haploid plant of S. cereale by Kostoff (1937), Nordenskiöld (1939) and Nakajima (1956, '57).
3. Trivalent was observed, though rarely, in addition to bivalents at the heterotypic metaphase, and tetravalent only once among 12600 PMC's.
4. In most individuals of the TperHRF1 plants used in this research, the meiosis of PMC's showed F1-type division just as in the Triticum-Secale F1 hybrids, but in two individuals (Tper HRF-1S-5, -S-17) all the 28 univalents were found to form the equatorial plate besides the F1-type division (Table 2).
5. All the 22 TperHRF1 plants investigated may be considered as, what might be called, the eu-trigeneric triple hybrid in which AB genomes of Triticum persicum, V genome of Haynaldia villosa and R genome of Secale cereale are completely included.
6. The distribution of chromosomes to opposite poles at the anatelophase in heterotypic division proceeded at random in F-type division, while in the case of the formation of equatorial plate some 28 chromosomes were distributed to each pole (Table 3).View full abstractDownload PDF (631K)
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