In the course of a study of
F elimination by acriflavine, a new mating type,
Fi (
i=intermediate), was induced in
F+ strains of
E. coli K-12.
Two types of
F+ were found; one having a tendency to spontaneously liberate
F- cells, the other preserving its
F factor firmly. It was found that acriflavine was especially effective in inducing
Fi and
F- from the former type.
F
i is distinguished from
F+ and
F- by the fact that it can mate with both
F+ and
F-, but is more fertile than
F+ in crosses with a standard
F+. Furthermore,
Fi behaves as a recipient to
F+, and as a donor to
F- in recombination processes.
The phenotypic components of an
Fi population are not uniform, but are composed of
F+ -like,
Fi and
F- cells. When the population is transferred serially in broth, it has a tendency to become either
F+-like or
F-, especially the latter, with gradual changes of its components.
From these facts, it was assumed that there might be an unstable condition, the
Fi state, between
F+ and
F-.
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