The irrigation efficacy of acidic water and a povidone iodine solution were examined in this study. Sterilized and distilled water was adopted as a control. Acidic water was produced using a special system (Asahi Glass Engineering Co. Ltd.). Before in vivo experiments, pH, oxidation-reduction potential, and potency of chloride ion (PCI) of Acidic water were tested in vitro to examine to what extent Acidic water will deteriorate under 6 different conditions of preservation in a capped bottle made of polyethylene terephthalate. The best results were obtained when acidic water was not exposed to air. During the testing period, the pH of the acidic water was unchanged. Heating to 40°C did not affect its nature. When acidic water was put in contact with animal skin or serum, PCI decreased. For in vivo experiments using seven cats, six skin lesions on the dorsal area of each animal were created aseptically, and a solution containing a measured number of
Pseudomonas aeruginosa was pored onto each wound as an infection model. Then the wounds were divided into three groups and they were respectively irrigated with acidic water povidone iodine solution, or distilled water. Activity of
Pseudomonas aeruginosa in each lesion was examined 24 hours after the irrigation. Macrophotographs of the lesions were taken and the size of each lesion was analyzed by computer. At biopsy, they were also examined for two weeks histopathologically. There were no differences between two tested groups. In the control, shrinkage rate of the wound showed the highest value 2 weeks after the start of the experiment. From these observations, we could not confirm the irrigation efficacy of the acidic water.
View full abstract