Blood concentrations of the vitamin B complex (B
1, B
2, B
6 and B
12) were measured in healthy cows both during the lactation period and during the non-lactation period, and in calves from 1 to 20 days after birth to determine the appropriate does of B complex supplement for dairy cattle. In the lactating group (n=5), the concentrations of the 4 vitamins measured were (Mean±SE) 51.2±1.2 ng/ml, 92.1±3.9 ng/ml, 37.8±1.7 ng/ml, and 220.2±16.3 pg/ml, respectively. In the non-lactating group (n=4), the 4 concentrations were 39.5±3.3 ng/ml, 75.2±4.6 ng/ml, 42.6±2.2 ng/ml, and 406.8±48.1 ng/ml, respectively. The levels of B
1 and B
2 were lower and the level of B
12 higher than in the lactating group (p<0.05). In the newborn calf group (n=9), the 4 concentrations were 47.0±3.6 ng/ml, 169.9±6.7 ng/ml, 4.1±0.2 ng/ml, and 357.4±44.2 ng/ml, respectivery. At 20 days after birth, they were 30.0±1.4 ng/ml, 130.9±6.4 ng/ml, 16.5±0.6 ng/ml, and 230.4±13.9 ng/ml, respectivery. With the exception of vitamin B
6, the level of the vitamin B complex decreased gradually in the postnatal period, On the basis of these data alone, it is difficult to determine supplemental doses of the vitamin B complex, but the data can be used as a reference in determining individual cases.
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