Embryological studies were made to clarify developmental processes, leading to seed collapse in crosses between diploid
Raphanus sativus (2
n=18) and its autotetraploid (2
n=36).
Hybrid embryos of reciprocal crosses appeared to develop normally in the early developmental stage, but in the cross, 2
x×4
x, they failed in general to develop beyond the globular stage because of degeneration of the endosperm. In the reciprocal cross, 4
x×2
x, viable hybrid seeds were produced, though their sizes were only one-fourth those of their parents.
In the 2
x×4
x cross, cell wall formation in the endosperm was inhibited, and abnormal vacuolization of endosperm occurred in all ovules, resulting in various nuclear irregularities and degeneration of ovules.
In the 4
x×2
x cross, the amount of endosperm was smaller but cell wall formation took place in it earlier than in parental endosperm. Development of the embryo was faster than in the parents.
A possible cause for the failure of viable seed formation was discussed in relation to the various abnormalities observed in various developmental stages.
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