巻号一覧
後続誌
25 巻 (1981 - 1982)
- 4 号 p. 243-
- 3 号 p. 155-
- 2 号 p. 79-
- 1 号 p. 11-
25 巻, 3 号
選択された号の論文の6件中1～6を表示しています
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井上 勤, 中田 郁枝1981 年 25 巻 3 号 p. 155-160
発行日: 1981/12/21
公開日: 2009/03/31
ジャーナル フリー日本産ミスジマイマイEuhadra peliomphalaの卵白腺と卵の可溶性蛋白質をデイスク・SDSおよびゲル等電点電気泳動法で研究した.
摂餌期では,卵白腺の蛋白質はデイスク電気泳動法で明瞭な10bandに分画されたが,冬眠期と夏眠期の両期では微量な16～20bandに分画された.卵白腺蛋白質パターンは季節変化と個体変異を示した.
卵白腺と卵の蛋白質はRm値が共に等しく,卵蛋白質は卵白腺から由来したものと考えられる.
卵蛋白質は分子量を74,000,94,000,125,000,156,000,200,000,400,000をもつ糖蛋白質で,その蛋白質はゲル等電点電気泳動法でpI3.5～9.0の21bandに分画された.
卵白腺のHCl加水分解物中の糖はgalactoseのみ検出された.抄録全体を表示PDF形式でダウンロード (2527K) -
血清,脊髄液,尿の蛋白質の分析真鍋 敬, 羽山 恵美子, 奥山 典生1981 年 25 巻 3 号 p. 161-166
発行日: 1981/12/21
公開日: 2009/03/31
ジャーナル フリーA technique of microscale-multisample two-dimensional polyacrylamide gel electrophoresis was described in detail. Proteins in human serum, cerebrospinal fluid and urine were separated by isoelectric focusing in cylindrical 4% polyacrylamide gel (T%=4.2, C%=5) of capillary size (i. d. 1.3mm× length 35mm) followed by 4-17% (T%=4.2-17.9, C%=5) acrylamide linear gradient electrophoresis using a slab gel of 38mm×35mm×1mm in size. This technique enabled to analyze eight body fluid samples within 5h.抄録全体を表示PDF形式でダウンロード (2635K) -
神田 進司, 真鍋 満久, 須藤 加代子, 菅野 剛史, 柴田 秀司1981 年 25 巻 3 号 p. 167-171
発行日: 1981/12/21
公開日: 2009/03/31
ジャーナル フリーAn electrophoretic separation and a quantitative staining method for leucine aminopeptidase, arylamidase and cystyl aminopeptidase were described. L-Leucinamide was used as a substrate for the aminopeptidases electrophoretically separated, and the liberated L-leucine was oxidized by leucine dehydrogenase and NAD+. The activity bands were visualized by tetrazolium salt (MTT) as a final hydrogen acceptor.
Arylamidase and cystyl aminopeptidase were stained in the area of pre α1- and between α1 and α2-globulin position, respectively. The leucine aminopeptidase was samely stained in the area of pre β-globulin. Employing this method, it was possible to determine the activity of each aminopeptidase with satisfactory accuracy and reproducibility.
Furthermore, this method would be useful to the analysis of enzymological properties of aminopeptidases because of its availability to other peptides, such as L-leucylglycine and L-leucyl-L-leucine, as substrate.抄録全体を表示PDF形式でダウンロード (3552K) -
渡部 昭子, 中山 年正, 北村 元仕1981 年 25 巻 3 号 p. 173-177
発行日: 1981/12/21
公開日: 2009/03/31
ジャーナル フリー1. Isozymes of LDH (lactate dehydrogenase, EC 1. 1. 1. 27) in serum and erythrocyte of 47 patients having very low serum LDH activity (lower than 160IU/l; normal range 195-359IU/l) was studied.
2. On the basis of the analysis of erythrocytes, 6 out of 47 cases (13%) were identified as the suspected cases of heterozygotes of hereditary LDH-H subunit deficiency.
3. With regard to serum LDH, it's stability in refrigerator was studied. Marked decline of LDH activity was observed in 4 out of 47 cases (9%). Two cases of these were the heterozygotes of LDH-H subunit deficiency.
4. We could not found any relation between the patients having lasting-low serum LDH activity and disease clinically diagnosed.抄録全体を表示PDF形式でダウンロード (559K) -
5'-AMP-Sepharose 4Bに対する親和性の差異園田 啓, 嵯峨 実枝子, 松本 宏治郎, 加野 象次郎1981 年 25 巻 3 号 p. 179-184
発行日: 1981/12/21
公開日: 2009/03/31
ジャーナル フリーIn this report, we described the differences of immunoglobulin-linked LDH in affinity to 5'-AMP-Sepharose 4B by use of the mini-column method and discussed them with respect to the kinetic properties.
Affinity of immunoglobulin-linked LDH whose electropherograms exhibited broad patterns (broad pattern type) was different from that of immunoglobulin-linked LDH with extra bands in enzymograms (extra band type).
In the cases of extra-band types due to either IgA- or IgG-linkage, LDH activities were not detected in the unadsorbed fractions and completely recovered in the NADH fractions of 5'-AMP-Sepharose 4B. Recovery of LDH activities derived from immunoglobulin-linked LDH was also confirmed by high speed liquid chromatography. On the other hand, in the cases of broad pattern types of IgG-linked LDH, LDH activities and those derived from IgG-linked LDH were eluted in the both two fractions.
IgA-linked LDH (extra band) could not be distinguished from normal sized LDH in their Km values for β-NADH, Km values of IgG-linked LDH (broad pattern) were observed slightly larger than those of normal sized LDH. Ki values of IgA-linked LDH (extra band) for 5'-AMP against β-NADH were intermediate between those of LDH-1 and LDH-5.
These results indicated that broad pattern types of IgG-linked LDH would have lower affinity to 5'-AMP-Sepharose 4B than extra-band types of immunoglobulin-linked LDH and normal sized LDH.抄録全体を表示PDF形式でダウンロード (629K) -
1981 年 25 巻 3 号 p. 185-235
発行日: 1981/12/21
公開日: 2009/03/31
ジャーナル フリーPDF形式でダウンロード (27466K)
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