生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
21 巻, 4 号
選択された号の論文の6件中1~6を表示しています
  • 易動度の変化について
    佐伯 進
    1978 年 21 巻 4 号 p. 249-257
    発行日: 1978/02/25
    公開日: 2009/03/31
    ジャーナル フリー
    Amylase isozyme analysis of serum, urine and other body fluids has been used in clinical diagnosis, in that identification of specific isozymes may permit identification of diseased organs. Contrary to what may be expected, the occurrence of multiforms of α-amylase and the changes in the electrophoretic mobility of amylase isozyme in serum and urine have been found. The present investigation was undertaken in an attempt to make clear the nature and the mode of the changes in the electrophoretic mobility of amylase isozyme, using electrophoresis on a thin layer polyacrylamide gel.
    Serum and urinary amylase isozymes of normal adult persons can be separated into two major isozymes (band-1 and -3) with high amylase activity and two or three minor ones with low amylase activity. Band-1 has the same electrophoretic mobility as the pancreatic isoamylase and band-3 is the same as the salivary isoamylase. The proportions of amylase activity of the individual isozymes can be altered, however, by prolonged storage in the cold or incubation at 37°C. The relative amylase activity of band-1 and -3 reduced and that of band-2 and -5 increased. After 5 to 7 days at 37°C, new bands of higher anodic mobilities come into being, the amylase activity of which increases in the course of time Thus, band-2, -4, -6 and -8 have appeared and increased in activity at the expense of band-1. Band-5, -7 and -9, not visible in the same amount of fresh sample have become to be seen at the expense of band-3.
    Neither bacterial contamination during prolonged storage, nor the addition of protease and trypsin had any effect on the changes of the banding pattern. Therefore, these factors could not be implicated as a cause of the changes in isozyme proportionality. On the other hand, trypsin-inhibitor and trasylol (Kallikrein-trypsin inhibitor) could not inhibit the transformation of isozymes into more anionic forms.
    Storage at alkaline pH and high temperature accelerated quantitative changes in the isozyme pattern. Addition of 0.1 mole CaCl2 and the storage below -18°C could retard the transformation of isoamylases.
    Column chromatographic investigation revealed that the molecular weights had not been significantly altered during storage or incubation at 37°C.
    These observations showed the difficulty of the interpretation of the isoamylase pattern, because the number of bands, as well as their relative amylase activities, may vary if the amylase activity, reaction time and conditions of the sample are not taken into account. Thus, artifacts formed during collection, storage or electrophoretic procedures and possible hereditary variants must be considered before coming to a conclusion of the pathological variants of the isoamylase pattern.
  • 三木 一正, 鈴木 宏, 丹羽 寛文, 遠藤 康夫, 飯野 四郎, 宮崎 純, 織田 敏次, 平野 和行, 杉浦 衛
    1978 年 21 巻 4 号 p. 259-266
    発行日: 1978/02/25
    公開日: 2009/03/31
    ジャーナル フリー
    We have reported that the properties of alkaline phosphatase (ALP) from meconium, neonatal feces and human fetal intestine, were similar to that of adult intestine except for their electrophoretic mobilites and sensitivities to neuraminidase, and they were considered not as one of isozymes but as one of modified enzyme of adult intestinal ALP, which were caused by the different content of sialic acid in them.
    We studied the properties of ALP from human fetal organs which were obtained from 13 fetuses, using the same technique as reported previously.
    The results were as follows: 1) Fetal intestine showed the heighest ALP activity of all organs, and that of bone showed the next. The activity of ALP of ovarium, adrenal, kidney, liver, lung, and spleen showed moderate activity, while that of testis, brain and stomach showed low activity. 2) According to their enzymological and immunological properties, ALP from human fetal organs were divided into two groups; one was hepatic-type ALP (ALP from liver, bone, spleen and so on), and the other was intestinal-type ALP (ALP of intestine). 3) The activity of ALP of fetal intestine increased markedly, and that of fetal thymus increased slightly during fetal development. 4) The properties of ALP of each fetal organs were similar to those of adult ones but the activity of ALP of fetal bone and adrenal, and the electrophoretic mobility and sensitivity to neuraminidase of ALP of fetal intestine were different from adult ones.
  • 増田 博, 七條 茂樹, 武内 睦哉
    1978 年 21 巻 4 号 p. 267-271
    発行日: 1978/02/25
    公開日: 2009/03/31
    ジャーナル フリー
    A study has been made of the glycosaminoglycans and glycopeptide in the abdominal walls of the normal rats. The abdominal walls were separated into three layers; skin, adipose tissue and muscle containing fascia, and then uronic acid, sulfate, hexose and sialic acid contents were measured in each layer. Values per g of wet tissue were highest in the dermis, while those of dry tissue were highest in the adipose tissue. Cellulose acetate strip-electrophoresis of concentrates in veronal butter revealed the presence of three alcian blue positive fractions in every layer, numbered in order of increasing electrophoretic mobility, fraction I, fraction II and fraction III; that is, fraction I corresponded in mobility to hyaluronic acid, fraction II to heparan sulfate, and fraction III to dermatan sulfate. The relative proportions of the fraction I, II and III were approximately 40%, 10% and 50%, respectively.
  • 1978 年 21 巻 4 号 p. 273-288
    発行日: 1978/02/25
    公開日: 2009/03/31
    ジャーナル フリー
  • 1978 年 21 巻 4 号 p. 289-331
    発行日: 1978/02/25
    公開日: 2009/03/31
    ジャーナル フリー
  • 島尾 和男
    1978 年 21 巻 4 号 p. 335-337
    発行日: 1978/02/25
    公開日: 2009/03/31
    ジャーナル フリー
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