巻号一覧
後続誌
42 巻, 2 号
選択された号の論文の13件中1～13を表示しています
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高月 清1998 年 42 巻 2 号 p. 73-77
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリーDNAからRNAへの読み取りとは逆方向の転写を行う酵素, すなわち逆転写酵素をもつウイルスを総称してレトロウイルスという. ヒトの疾患の病因ウイルスとしてはHTLV-1とHIVがある. それぞれ成人T細胞白血病 (ATL) とエイズの病原体である. ATLは日本で発見記載された疾患であり, 九州, 沖縄などに多い. エイズは1980年代に全世界に拡がり人類の脅威として恐れられている. この2つの疾患について疫学, 病像, 診断, 検査, 予防と治療を解説した. 現代の医学ではまだ解決しないが, 研究の進歩により曙光のみえてきた部分もある.抄録全体を表示PDF形式でダウンロード (594K) -
清水 章1998 年 42 巻 2 号 p. 79-85
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリーDirect examination of hemolysate by electrospray ionization mass spectrometry may lead to the rapid ascertainment of variant hemoglobins (Hb) and to the quantification of glycated Hb. Plasma and cell proteins other than Hb can be prepared by immunoprecipitation prior to MS. The detected variants were sequenced by MS using enzyme cleaved peptides. We detected various variants of Hb, transthyretin, and CuZn-superoxide dismutase. New variants included; 1) Hb Sagami [β139 (H17) Asn→Thr], and 2) Hb Hokusetsu [β52 (D3) Asp→Gly]. These were studied to clarify the cause of unusual profiles of HPLC for HbAlc measurement. Hb Sagami was an electrophoretically silent and isopropanol test-negative variant, detected solely by ESIMS. Other new variants included; 3) a variant transthyretin, amyloidogenic, [38 Asp→Ala], and 4) another variant transthyretin, non-amyloidogenic, [101 Gly→Ser]. Variant transthyretins are usually difficult to detect by electrophoresis. Samples containing variant Hb or high carbamylated Hb gave erroneous results of glycated Hb by routine HPLC and immunoassay, but did not by MS.抄録全体を表示PDF形式でダウンロード (2460K) -
宮崎 香, 越川 直彦1998 年 42 巻 2 号 p. 87-92
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリーTumor cells produce various types of matrix-degrading proteinases which play essential roles in tumor cell invasion through basement membranes and connective tissues. Highly sensitive analysis of proteinases by zymography on gelatin- or casein-containing gels has shown that tumor cells frequently secrete matrix metalloproteinases (MMPs) including gelatinases A and B, interstitial collagenase, stromelysin and matrilysin, as well as the serine proteinase trypsin. On the other hand, analysis by reverse zymography has revealed that tumor cells also secrete various kinds of inhibitors for MMPs and trypsin. These results suggest that extracellular proteolysis has been regulated by a balance between various kinds of proteinases and their inhibitors. The zymography and reverse zymography can be widely applied to studies on proteinases present in various biological fluids and tissues.抄録全体を表示PDF形式でダウンロード (4039K) -
ゲルシフト法河邊 拓己, 手塚 俊文, 岡本 尚1998 年 42 巻 2 号 p. 93-96
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリーDNA binding proteins play important role in the DNA replication, recombination, transcription and the viral integration to genome. Several methods to analyse these DNA binding proteins such as methylation interference and DNase protection analysis were developed. Among them gel shift analysis has been widely used because of its simplicity, high sensitivity and rapidity. We review the detail of this method by refering to the example of the analysis of the redox regulation of the transcription factor NF-κ B binding to DNA. In this analysis the DNA binding activity of the NF-κB was abolished by the treatment with the oxidizing reagent diamide. Thioredoxin (ADF), but not glutathione efficiently activated NF-κB binding activity to its consensus DNA sequence.抄録全体を表示PDF形式でダウンロード (4066K) -
手塚 俊文, 河邊 拓己, 岡本 尚1998 年 42 巻 2 号 p. 97-101
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリーThe phosphorylation and/or dephsophorylation of transcription factors modulate their nuclear translocation and/or transcriptional activities. The in-gel kinase assay was used to identify the kinases which phosphorylate transcription factors, c-Jun and NF-κB. Recombinant c-Jun or NF-κB was copolymerized in an SDS-PAGE gel as a substrate. After electrophoresis, the gel was denatured in 6M guanidine and then renatured in a buffer containing 0.04% Tween 40. The renatured substrates and kinases in the gel were incubated with [γ-32P] ATP. The renatured kinases successfully phosphorylated transcription factors. The in-gel kinase assay revealed that interleukin-1 stimulates c-Jun aminoterminal kinase (JNK). This method also showed that c-AMP dependent protein kinase (PKA) phosphorylates the p 65 subunit of NF-κB. Thus the in-gel kinase assay is a useful method to identify the kinases which phosphorylate transcription factors.抄録全体を表示PDF形式でダウンロード (2719K) -
波田 寿一1998 年 42 巻 2 号 p. 103-108
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリー正常人アルカリフォスファターゼ(AP)は肝/骨/腎AP, 胎盤AP, 小腸AP, 生殖細胞APの4種類に分類される. 5%PAGEでヒト血清APのアイソザイム(I)を調べると, 陽極側から肝AP, 骨AP, 胎盤AP, 小腸APの順に泳動されるが, われわれは肝癌患者血中に肝APよりさらに陽極側に泳動されるAPIを見いだし, 酵素学的, 免疫学的性質の検討から, 本酵素は胎盤APの変異型と考え variant APとして報告した. その後, FL-羊膜細胞が有する2つのAPIのうちの1つと variant APが同じ性質を有することを明らかにし, 最初の患者の名にちなんで Kasahara isozyme(K.I.)と命名した. ここでは, K.I. の酵素学的, 免疫学的性質, 遺伝子の検討, 糖鎖構造, 臨床的意義, 病理学的知見等について紹介した.抄録全体を表示PDF形式でダウンロード (2041K) -
高木 康1998 年 42 巻 2 号 p. 109-112
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリーCreatine kinase (CK, EC 2.7.3.2) has a important role in muscle contraction and is rich in muscle tissue. The CK has three isoenzymes, designated as CK-MM (also termed CK3), CK-MB (CK2) and CK-BB (CK1). These isoenzymes are clearly separated by an electrophoresis. Myocardium contains significant quantities of CK-MB. Demonstration of elevated levels of CK-MB is considered the most specific indicator of acute myocardial infarction (AMI). CK-MM is resolved into three isoforms (MM1, MM2, MM3) by prolonged electrophoresis. The CK-MM3 in myocardium is released into blood and changes to CK-MM2, then CK-MM1 post-translationally. The detection of an elevated percentage of CK-MM3 in serum of patients with AMI indicates only a relatively recent myocardial injury like a myoglobin. In recent years, the unusual CK isoenzyme bands displaying electrophoretic properties that differ from the major isoenzyme fractions were observed. These atypical forms are generally of two types and are referred to as CK-linked immunoglobulin and mitochondrial CK (CK-m). The CK-m is detected in serum of extensive tissue damage, causing breakdown of the mitochondria.抄録全体を表示PDF形式でダウンロード (3969K) -
片山 善章, 米田 孝司1998 年 42 巻 2 号 p. 113-116
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリーThe change of mitochondria aspartate aminotransferase (mAST)/soluble AST (sAST) ratio was examined in 22 cases of acute myocardial infarction (AMI). The mAST/sAST was 0.408±0.189 at admission to a hospital (2.9±1.6h). The mAST/sAST decreased to normal value rapidly after peak and then increased again gradually. The decrease ratio of mAST/sAST per minute at early stage of 8 cases, who were succeeded to reperfusion, was 0.28±0.20, and that was significantly higher than of conventionally treated 7 cases and non-reperfused 7 cases (0.11±0.07). These results indicated that (1) mAST/sAST may be an excellent indicator for AMI in early stage. (2) The decrease ratio of mAST/sAST would predict whether reperfusion is successful or not at an earlier stage of AMI.抄録全体を表示PDF形式でダウンロード (323K) -
林 泰三1998 年 42 巻 2 号 p. 117-122
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリーSerum PGAM activities and the percentage of brain-subunit activity against total activity (B/T) were determined in the cases of apoplexy and traumatic injury on head. PGAM activities already increased and peaked whthin 2h after the stroke. This findings were also recognized in the cases with head-trauma. They gradually decreased in 12h, 24h and 3 day after the attack. The grade and duration on the elevation of PGAM activities were more dominant in the serious cases than in the slight cases. In these cases with apoplexy and head-traum, B/T ratio almost remained in the normal range, namely about 90%. In the CSF, PGAM activities were consist of B-subunit, and significantly correlated with LD-activities and the protein content. In the experiment on SHRSP, serum PGAM activities were markedly increased during the stroke, compared with that of control rat. We suggest that serum PGAM activities would be an useful marker of apoplexy and the other brain damage.抄録全体を表示PDF形式でダウンロード (704K) -
酒井 伸枝, 芝 紀代子, 平塚 信夫, 保崎 清人, 西田 賢司, 椎名 幸恵, 芝 〓彦1998 年 42 巻 2 号 p. 123-129
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリーSDSを含む緩衝液を用いたキャピラリー電気泳動により, 尿成分を分析する方法を考案した. この方法を用いて, 健常人をはじめ糖尿病患者のうち, 腎症前期症例, 早期腎症例について尿分析を試みた. その結果, 健常人では44, 腎症前期例では46, 早期腎症例では53のピークが検出された. それらのピークのうち10本のピークが同定された. CE-SDS標準蛋白分子量マーカーを用いたところ, 分子の大きさと分離時間との間には比例の関係がみられた. しかしながら, 糖鎖含有量の高いα1-AGやTHP蛋白では, 実際の分子の大きさよりも分離時間が遅く, 見かけ上分子量が大きく求められることがわかった. 腎障害の進行に伴い, 高分子の成分が増加する傾向がみられた. また, 腎症前期例と早期腎症例では出現するピークも相違がみられることから, 各成分の同定をさらに行うことにより, 糖尿病患者における早期腎症の指標となりうることが示唆された.抄録全体を表示PDF形式でダウンロード (692K) -
森山 隆則, 戸沢 辰雄, 平田 哲, 池田 久實1998 年 42 巻 2 号 p. 131-136
発行日: 1998/06/15
公開日: 2009/03/31
ジャーナル フリーElectrophoretic and chromatographic characterization of macroamylasemia induced by hydroxyethyl starch (HES) was performed. HES-induced macroamylasemic sera were obtained from the patients subjected to intravenous infusion of HES solution during surgery. Macromolecular amylase was observed in the patient's sera obtained from termination of the infusion of HES to 24h post-operation by gel-permeation high-performance liquid chromatography (HPLC) analysis. However, anomalous bands were detected in the patient's sera only immediately or two hours after termination of the infusion by isoamylase electrophoretic analysis. In the samples of non-macroamylasemic serum mixed with HES solution in vitro, the anomalous pattern was observed only in the sample with volume ratio of 1:1 and/or 1:1/2. In contrast, the peak of macromolecular amylase was detected in the all samples with the ratio from 1:1/2 to 1:1/20. From the results, it was recognized that gel-permeation HPLC analysis was a more sensitive method in HES-induced macroamylasemia than isoamylase electrophoretic analysis.抄録全体を表示PDF形式でダウンロード (3206K) -
1998 年 42 巻 2 号 p. 139a
発行日: 1998年
公開日: 2009/03/31
ジャーナル フリーPDF形式でダウンロード (84K) -
1998 年 42 巻 2 号 p. 139b
発行日: 1998年
公開日: 2009/03/31
ジャーナル フリーPDF形式でダウンロード (84K)
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