It has been pointed out that patterns of polychlorobiphenyls (PCB) in the blood of “Yusho” (polychlorobiphenyl-poisoning) patients differ from those of normal adults.
Gaschromatography using a 5m column packed with 2% Apiezon L showed that the relative concentrations of 3, 4, 2′, 4′, 5′-pentachlorobiphenyl (peak 25) and 3, 4, 2′, 3′, 4′-pentachlorobiphenyl (peak 28) in the blood of Yusho patients were lower than in normal adults; this was considered to be due to coexisting polychlorodibenzofurans (PCDF) in Yusho oil.
To clarify this, PCB and PCDF fractions were separated from Kanemi rice oil (Yusho oil) which had caused Yusho.
Yusho oil, rice oil with PCB (154ppm) or rice oil with PCB (154ppm) +PCDF (2ppm) were given orally to different groups of mice at a dose of 0.1ml per body per day for 9 days, and PCB residues in the adipose tissue and liver were examined on the 1st, 7th and 35th days after the termination of dosing.
The characteristic PCB patterns of Yusho patients were obtained from the groups given Yusho oil and PCB+PCDF, but not from the group given PCB alone.
Less chlorinated biphenyls, peak 25 and peak 28, decreased with time during the experiment, but 3, 4, 2′, 3′, 4′, 5′-hexachlorobiphenyl (peak 47) and 2, 4, 5, 2′, 3′, 4′, 5′-heptachlorobiphenyl (peak 49) increased.
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