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Volume 36 (1995)
- Issue 6 Pages 699-
- Issue 5 Pages 583-
- Issue 4 Pages 477-
- Issue 3 Pages 329-
- Issue 2 Pages 219-
- Issue 1 Pages 1-
Volume 36, Issue 3
Displaying 1-16 of 16 articles from this issue
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Masakazu HORIE, Hiroyuki NAKAZAWA1995 Volume 36 Issue 3 Pages 329-343
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (1890K) -
Masataka SATOMI, Toshiki YAMAGUCHI, Masayo OKUZUMI, Tateo FUJII1995 Volume 36 Issue 3 Pages 344-351_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe effect of various conditions on the recovery of pressure-injured Escherichia coli and Vibrio parahaemolyticus was examined. Bacteria were pressurized at 2, 000atm for 30min (E. coli) or 1, 200atm for 10min (V. parahaemolyticus) at room temperature. The treated bacteria were then suspended in several kinds of solutions for recovery, such as nutrient medium, minimum medium and 50% artificial sea water (A. S. W.) and viable bacteria were counted on Trypticase Soy Agar (TSA) and an appropriate selective medium. Effects of temperature, pH and NaCl concentration on recovery were also examined. Favorable conditions for E. coli and V. parahaemolyticus were recovery in nutrient medium of less than 1.0% NaCl concentration, pH 7.0, at 30-37°C, and aerobic recovery in nutrient medium of 0.5 to 3.0% NaCl concentration, pH7.0, at 37°C, respectively.View full abstractDownload PDF (794K) -
Tomoko HAYASHI, Miwa WATANABE, Yukiyo FUKAYA, Kiyoko YOSHIDA, Kazunori ...1995 Volume 36 Issue 3 Pages 352-359_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple, rapid, and reliable method for simultaneous determination of 12 unusualsmelling substances (benzene, ethyl benzene, n-propylbenzene, isopropylbenzene, toluene, o-xylene, m-xylene, p-xylene, styrene, o-dichlorobenzene, m-dichlorobenzene, and p-dichlorobenzene) in foods has been developed. The substances were extracted from foods by using an essential oil distillator and the extract, containing tetradecane as an internal standard, was analyzed by gas chromatography with FID. Recoveries of the 12 substances except benzene from 10 commercial foods fortified at the level of 50ppm were more than 85%, coefficients of variation were less than 5%, and the detection limits were 1ppm. The present method was successfully applied to the determination of styrene in a sample of cooked brown algae (Hijiki-no-tukudani) which had been reported to a health center by a dissatisfied consumer due to its unusual smell.View full abstractDownload PDF (834K) -
Kayoko USHIJIMA, Yoshiko DEGUCHI, Kouji KIKUKAWA, Takakazu NOMURA, Tad ...1995 Volume 36 Issue 3 Pages 360-364_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method for analysis of residual 3-chloro-1, 2-propanediol (MCP) in seasonings was developed by capillary gas chromatography-mass spectrometry (selected ion monitoring method). MCP was extracted with ethyl acetate through an Extrelut® 20 column. The eluate was concentrated and derivatized with phenylboronic acid. The calibration curve showed good linearity in the range of O.01-4ng.
The recoveries of MCP spiked at 2ppm in liquid seasoning and 20ppm in solid or viscous seasoning were in the range of 96-102%. The limits of determination were 0.005ppm for liquid seasoning and 0.05ppm for solid or viscous seasoning.
This method is simple, rapid and applicable for microanalysis of residual MCP in seasonings.View full abstractDownload PDF (513K) -
Susumu KUMAGAI, Anong BINTVIHOK, Mari KONO, Masaaki IWAKI, Yoshiko SUG ...1995 Volume 36 Issue 3 Pages 365-374_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSActivation and inactivation of aflatoxin B1 (B1) by microsomes and cytosol prepared from the liver of various mammalian and avian species were studied in vitro by determining the microsomal activity to bind aflatoxin to calf thymus DNA and the cytosol activity to inhibit the hamster microsome-mediated aflatoxin-DNA binding. The microsomal activity to bind aflatoxin to DNA was higher in day-old duckling and female chicken than in the other species, being similar in the male hamster, male chicken, both sexes of Japanese quail and laying duck, and lower in the male rat and male mastomys than in the other species. The hamster cytosol inhibited the aflatoxin-DNA binding markedly in the presence of glutathione (GSH) but not at all in its absence. In contrast, the avian cytosol showed a similar level of aflatoxin-DNA binding regardless of the presence or absence of GSH, suggesting that the contribution of cytosol glutathione S-transferase (GST) to B1 detoxification is negligible in the avian species. Nevertheless the cytosol of the avian species such as the Japanese quail and chicken showed apparent inhibitory activity toward aflatoxin-DNA binding. Relative susceptibility of the mammalian and avian species to the toxic and carcinogenic effects of B1 could be explained by the combined activities of microsomes and cytosol, indicating the importance of the opposite activities of microsomes and cytosol toward aflatoxin-DNA binding in the species difference in susceptibility.View full abstractDownload PDF (1127K) -
Yasushi SEKIYAMA, Yuichi MIZUKAMI, Asami TAKADA1995 Volume 36 Issue 3 Pages 375-382_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAllyl isothiocyanate (AIT), the main flavoring component of wasabi and mustard, is reported to have strong antimicrobial activity. A study was made of the corrosive effects of AIT vapor on various substances such as metals and rubbers, and of its ability to permeate food packaging films.
It was found that allyl isothiocyanate vapor had no corrosive effect at concentrations of less than 100ppm. At the high concentration of 3, 000ppm, however, corrosion was observed in neoprene rubber, and in all plastics except polyolefin resins, polyacetal, nylon and Teflon.
AIT was found to have a moderate ability to permeate food packaging films made of polyethylene and polypropylene, with permeability inversely proportional to film thickness.View full abstractDownload PDF (732K) -
Toshihiro NAGAYAMA, Maki KOBAYASHI, Hiroko SHIODA, Masako ITO, Yukihir ...1995 Volume 36 Issue 3 Pages 383-392_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSPesticide residues in or on fruits and translocation of pesticide from the peel to the flesh were investigated.
Except for water-soluble pesticides, the highest ratio of the concentration in the flesh to that in the peel was found with imidazole and phenoxy pesticides, followed by carbamate pesticides. Organochlorine and organophosphorus pesticides gave low ratios.
The regression equation log (Cf)=-1.22+0.924×log (Cp) (γ=0.892), where Cf is the pesticide concentration in flesh (ppm) and Cp is that in peel (ppm), was obtained for the relation between imazalil residues in lemon peel and flesh. The relation between the concentration ratio of the flesh to the peel and the pesticide solubility in water was given by: Cr=-0.99+2.41×log (Sw) (γ=0.942), where Cr is the concentration ratio (%) in the flesh and the peel and Sw is the pesticide solubility in water (mg/L).View full abstractDownload PDF (946K) -
Mitsuo NAKAZATO, Kazuo SAITO, Takako MORIYASU, Fusako ISHIKAWA, Kenji ...1995 Volume 36 Issue 3 Pages 393-399_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe Japanese product “Kusaya” is made from fishes (mackerel-scad, flying fish, etc.) through processes of fermentation and drying. A survey of volatile basic nitrogen (VBN) and non-volatile amines, putrescine (Put), cadaverine (Cad), histamine (Him), tyramine (Tym) and spermidine (Spd), was carried out on 29 Kusaya samples which consisted of 12 broiled, 15 seasoned and 2 other products.
VBN in the broiled products ranged from 220 to 520mg%, and that in the seasoned products ranged from 77 to 510mg%. In the broiled products, Put and Spd were detected in 8 samples, but the amounts were low: from 1.0 to 2.3μg/g and from 1.1 to 7.7μg/g, respectively. In the seasoned products, five non-volatile amines were detected at higher levels and with a higher frequency than in the broiled products. The amounts of Put, Cad, Him, Tym and Spd were 1.0-110, 1.0-380, 10-530, 9.1-67 and 1.2-5.5μg/g, respectively.
Changes in the amounts of the five amines and VBN during the manufacture of Kusaya and Kusaya products were examined. The results imply that: (1) in the broiled products, Put originated from soakage fluid at the fermentation stage, whereas Spd was derived directly from the raw fish; (2) most of the five amines in seasoned products originated from soy sauce used in the seasoning; (3) VBN in both products originated from the soakage fluid.View full abstractDownload PDF (927K) -
Toshihiro NAGAYAMA, Maki KOBAYASHI, Hiroko SHIODA, Masako ITO, Yasuhir ...1995 Volume 36 Issue 3 Pages 400-403_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSBitertanol residues in 109 banana samples were investigated. Most of the samples contained residues below the allowed level. The levels of bitertanol residues in 2 samples were higher than the maximum limit of 0.5ppm, but little had translocated to the pulp.
Great variations in the levels of bitertanol residues were observed within clusters of bananas, or even single bananas, and therefore careful attention must be paid to ensure adequate sampling. Bitertanol residues did not decrease within the period from importion through ripening, to sale.View full abstractDownload PDF (403K) -
Naoko ISHIWATA, Akio TANIMURA, Michiko MIYAHARA, Katsutoshi MISE1995 Volume 36 Issue 3 Pages 404-408_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn Hsd (host specificity for DNA) plasmid designated pSTd4 was found in Salmonella Typhi D4, one of the standard phage typing strains. The plasmid was introduced into E. coli K-12, and the restriction map of this plasmid was constructed. The efficiency of plating of λ·0 phage was drastically reduced with pSTd4 as well as other small Hsd plasmids of Enterobacteriaceae origin. The results clearly indicate that pSTd4 is a type-determining plasmid in S. Typhi D4. We propose that rare phage types such as D4 carrying typedetermining plasmids or lysogenic phages should be eliminated from the collection of standard phage typing strains of S. Typhi.View full abstractDownload PDF (4293K) -
Takao MITSUHASHI1995 Volume 36 Issue 3 Pages 409-412_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn this study, the alkaline ashing and derivatization conditions of our previous gas chromatographic determination of total bromine using 3-pentanone derivatization were improved for the analysis of agricultural products.
A 2g sample (for cereals and beans), or a 5g sample (for vegetables and fruits) was ashed with 5ml of 5% potassium hydroxide in ethanol-water (1:1) mixture at 550°C for 5 hours. Bromide in a water extract of the ash residue was converted into the bromo derivative of 3-pentanone (BP), and then determined by capillary gas chromatography with an electron-capture detector. The addition of sulfamic acid to a water extract increased the peak height of BP, and suppressed the formation of the chloro derivative of 3-pentanone.
Recoveries of bromide from agricultural products spiked at 5μg/g ranged from 87.2 to 96.8%. The detection limits were 0.05μg/g for cereals and beans, and 0.02μg/g for vegetables and fruits.View full abstractDownload PDF (489K) -
Hiroyuki NAKAGIRI, Shuso SAKUMA, Masahiro KOWAKA1995 Volume 36 Issue 3 Pages 413-416_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSOccasionally, insects are detected in bottles of beer on the market. When insects are detected in pasteurized beer, we are able to determine whether they entered the bottle during the packaging process or after the bottle was unstoppered by measuring the catalase activity of the insect. However, this method is inaccurate and impractical for insects in draught beer. We have investigated and developed a new analytical method. This new method can determine the number of days an insect has been in the beer by measuring the cholinesterase activity remaining in the insect. The cholinesterase activity varies little with the individual. This method is very simple, requiring only a homogenizer, spectrophotometer, centrifuge, and reagents.View full abstractDownload PDF (380K) -
Makiko YAMADA, Yoshiaki KATO, Mikio NAKAMURA, Susumu ISHIMITSU, Tadash ...1995 Volume 36 Issue 3 Pages 417-422_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method for determination of the starting materials, [7-hydroxy-1, 3, 6-naphthalenesulfonic acid (TS), G-salt (GS), R-salt (RS), Schaeffer's salt (SS) and naphthionic acid (NA)] and subsidiary colors, [ponceau 6R (P6R), amaranth (R-2) and fast red E (FRE)] in new coccine (R-102) was developed by use of HPLC. The following conditions were used for analysis: column, L-column ODS (4.6mm i. d.×250mm); eluent, gradient elution system of 0.02M ammonium acetate and acetonitrile (hold at 0.02M ammonium acetate for 5min, then 0-30% acetonitrile in 45min); detection for TS, GS, RS, SS and NA at 238nm and subsidiary colors at 510nm.
Recoveries of each impurity from R-102 averaged 99.1-103.5%. Ten samples of commercial R-102 were analyzed by the present HPLC method and the impurites were as follows: TS, 0.005-0.044%; GS, 0.044-0.284%; NA, 0.013-0.196%; RS, N. D.; SS, N. D.; P6R, 0.008-0.169%; R-2, N. D. -0.279%; FRE, 0.007-0.100%. The detection limit was 0.001% for each impurity.View full abstractDownload PDF (603K) -
Mari MIZUTA, Kumiyo TAKATA, Tatsuhisa MONDEN, Tatsuo YONEDA, Shinya YA ...1995 Volume 36 Issue 3 Pages 423-427_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS -
Sumiko TSUJI, Chikako YOMOTA, Tadashi SHIBATA, Kenji ISSHIKI, Mieko KA ...1995 Volume 36 Issue 3 Pages 428-441_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS -
Yutaka MINEGISHI, Yasuyuki TSUKAMASA, Kiyotaka MIAKE, Tsukasa SHIMASAK ...1995 Volume 36 Issue 3 Pages 442-446_1
Published: June 05, 1995
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS
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