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Volume 40 (1999)
- Issue 6 Pages 417-
- Issue 5 Pages 335-
- Issue 4 Pages 259-
- Issue 3 Pages 183-
- Issue 2 Pages 123-
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Volume 40, Issue 4
Displaying 1-11 of 11 articles from this issue
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Detection by Using Monolayer-cultured CellsMakoto SHIMIZU, Tomoyukl OKADA1999 Volume 40 Issue 4 Pages 259-265
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (1139K) -
Jianen Hu, Yasurou KURUSU, Masakazu TSUTSUMI1999 Volume 40 Issue 4 Pages 266-273_1
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe antibacterial action of glycine and some derivatives on E. coli was investigated in the presence of ethanol. (1) The growth of E. coli was inhibited by glycine and slightly by alanine, but not by glycinamide or sarcosine. (2) The activity of glycine was strong at low temperature (20°C) or at alkaline pH (pH 9.0), and weak at high temperature (40°C) or at acidic pH (pH 5.0). (3) Synergistic action of glycine and ethanol was observed. (4) E. coli cells were injured by glycine and/or ethanol, and their ability to form colonies on deoxycholate agar was lost. However, the colony-forming ability of the injured cells was restored by incubation in recovery broth. (5) Though the respiratory activity of the cells was inhibited by treatment with these chemicals, this activity was also restored when the injured cells were incubated in suitable broth. However, the mode of recovery of respiratory activity of the cells may be different from that of the colony-forming ability.View full abstractDownload PDF (988K) -
Yoko KAWAMURA, Chieko TAGAI, Tamae MAEHARA, Takashi YAMADA1999 Volume 40 Issue 4 Pages 274-284_1
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe residue of 89 kinds of additives including plasticizers in polyvinyl chloride (PVC) and polyvinylidene chloride (PVDC) products were investigated. PVC wrapping films contained 14-38% of plasticizers, most of which were adipates, especially diisononyl adipate. However, few additives were observed in PVC containers. PVC gloves contained 34-55% of plasticizers, mostly di-2-ethylhexyl phthalate and di-2-ethylhexyl adipate, in all samples. The levels of plasticizers in toys were 8.7-45%, consisting mainly of diisononyl phthalate and di-2-ethylhexyl phthalate. On the other hand, the levels of plasticizers in PVDC wrapping films were only 2.7-7.8% (O-acetyl tributyl citrate and dibutyl sebacate). Nonylphenol was found in some PVC wrapping films, gloves and toys at concentrations of about 530-5, 500μg/g sample.View full abstractDownload PDF (1204K) -
Masanori NAKASHIMA, Akiko SUGIYAMA1999 Volume 40 Issue 4 Pages 285-290_1
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA rapid and simple method was developed for quantitative analysis of histamine in fish using capillary electrophoresis (CE). Samples were homogenized, extracted with 5% trichloroacetic acid, filtered, and subjected to CE. Detection was achieved with a UV detector set at 210nm. Identification was done from the absorption spectra obtained with a photodiode array detector. This method was able to analyze histamine without a purification procedure or fluorescence derivatization, both of which are required in the HPLC method. Reproducibility of migration time and peak area were <0.37% and <4.6%, respectively, for histamine analysis (RSD, n=5). Determination range was 50-10, 000mg/kg, and the correlation coefficient of the results with those obtained by HPLC was 0.996 (n=12).View full abstractDownload PDF (613K) -
Kiyomi ASAKA, Yukie SEKIGUCHI, Miho ARAI, Akira KAWABATA, Masaru SAITO ...1999 Volume 40 Issue 4 Pages 291-296_1
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSStaphylococcus aureus in nostril anteriors of 199 trade school students was surveyed 8 times from May 1997 through February 1998. A survey by questionnaire for their history of allergic diseases was also performed. S. aureus were isolated at least once from 73 students and not at all from 126 students. Of the 73 students, 55 were temporary carriers, while S. aureus was always found in 18 students. The correlation between S. aureus carrier status and history of allergic diseases is significant at the 0.1% level (Yates x2 test).View full abstractDownload PDF (721K) -
Growth and Inhibition by Acids of Five Species of Pathogenic Bacteria Inoculated in Salad VegetablesMakoto OHSONE, Ken-ichi KANEKO, Hideki HAYASHIDANI, Toshihiro TAKAHASH ...1999 Volume 40 Issue 4 Pages 297-303_1
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe growth of Yersinia, Listeria monocytogenes, Escherichia coli O157, and Staphylococcus aureus in salad vegetables stored at 5°C, 10°C, and 25°C, and the initial reduction of the bacterial populations by treatment of the vegetables with organic acids were examined. The multiplication of Yersinia enterocolitica was observed in cabbage and radishes held at 5°C for 4 days, at 10°C for 2 to 3 days, and at 25°C for 1 day. That of Yersinia pseudotuberculosis was observed in radishes at 5°C for 3 days, at 10°C for 3 days and at 25°C for 1 day. That of L. monocytogenes was observed in radishes at 10°C for 3 days, and in cabbage and carrots stored at 25°C for 1 day. That of E. coli O157 was observed in cucumbers held at 10°C for 3 days and in all five kinds of vegetables held at 25°C for 1 day. Reduction of L. monocytogenes, E. coli O157, and S. aureus by log 2 cycles or more was observed within 2 days after treatment with fumaric acid. Following treatment with acetic acid and lactic acid, the reduction of the initial population of these bacteria was about log 1 cycle.View full abstractDownload PDF (969K) -
Eiko AMAKAWA, Kunihiro KAMATA, Kazuo SAITO, Sukeji SUZUKI1999 Volume 40 Issue 4 Pages 304-308_1
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDetermination of alitame in food stuffs by TLC and HPLC was studied. Alitame was extracted with water-acetonitrile (8:2) from food stuffs. For samples containing a large amount of sugar, the extracted solution was cleaned up using a DEAE-Sephadex column. The extracted solution was poured onto the column, then the column was washed with water, and alitame was eluted with 0.2mol/L hydrochloric acid in 20% methanol containing methyl red. Alitame was detected by TLC, and determined by HPLC. TLC conditions: plate, RP-18F254S; eluent, 0.02mol/L potassium dihydrogenphosphate solution (pH 4.6)-methanol (4:6); visualization, heating at 90°C for 15min after spraying 0.3% ninhydrin solution. HPLC conditions: column, Lichrosorb RP-18-5; mobile phase, 0.02mol/L potassium dihydrogenphosphate solution (pH 4.6)-acetonitrile (8:2); detection, 210nm; column temperature, 45°C; flow rate, 1.0mL/min; injection, 10μL. Recoveries of alitame added to commercial foods were 99.0-104.2% and the detection limit was 20μg/g.View full abstractDownload PDF (577K) -
Masakazu HORIE, Rie ISHII, Terumitsu YOSHIDA, Youji HOSHINO, Hiroyuki ...1999 Volume 40 Issue 4 Pages 309-313_1
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple and rapid method using liquid chromatography-electrospray ionization-mass spectrometry (LC/MS-ESI) has been developed for the determination of macrolide antibiotics, erythromycin and oleandomycin, in meat and fish. The LC separation was carried out on a TSK-gel ODS 80-Ts column (150×2mm) by using 0.1% acetic acid-acetonitrile (65:35) as the mobile phase at a flow rate of 0.2mL/min. The positive ionization produced typical (M+H)+ molecular ions of both drugs (erythromycin m/z 734; oleandomycin m/z 688). The calibration graphs for erythromycin and oleandomycin were rectilinear from 0.05 to 10ng with selected ion monitoring (SIM). The drugs were extracted with 0.1% metaphosphoric acid-methanol (5:5), and the extracts were cleaned up on an OASIS HLB cartridge (60mg). The recoveries of the drugs from meat and fish fortified at a level of 0.2μg/g were 79.4-95.4%, with high precision. The limits of quantification of both drugs in meat and fish were 0.01μg/g.View full abstractDownload PDF (624K) -
Satoshi TAKATSUKI, Satoru NEMOTO, Rieko MATSUDA, Kumiko SASAKI, Masata ...1999 Volume 40 Issue 4 Pages 314-319_1
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA screening method using HPLC for the simultaneous determination of 21 pesticides in agricultural products has been developed. The pesticides were extracted from samples with acetonitrile. The extract was cleaned up on a gel permeation column and a silica gel cartridge. The silica gel cartridge was eluted with 5% acetone-hexane (A/H), 35% A/H, 60% A/H, and 30% methanol-acetone, successively. The eluates were concentrated and analyzed on a high performance liquid chromatograph equipped with a photodiode array detector, using an Inertsil ODS-2 column (150×4.6mm i. d.) with a gradient system of 0.003mol/L phosphoric acid-acetonitrile as the mobile phase at a flow rate of 1.0mL/min.
The recoveries at the level of 0.4μg/g were 62-120%, except for bentazon in banana and quinclorac in 5 crops tested. Detection limits were 0.005-0.02μg/g for the 21 pesticides.View full abstractDownload PDF (669K) -
Yukio TANAKA, Shinjiro HORI1999 Volume 40 Issue 4 Pages 320-324_1
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSWith a view to establishing a microanalytical method for boron in foods, we investigated inductively coupled plasma (ICP)-atomic emission spectrometry, a new analytical method substituting for curcumine colorimetric analysis, which has been widely used.
Ashing for pretreatment was examined using polished rice and milk as samples. Boron did not volatilize during dry ashing without addition of sodium carbonate solution. Comparison of analytical values obtained with the proposed ICP-atomic emission spectrometry and curcumine colorimetric analysis indicated a good coincidence. However, analytical precision was much higher for the former analysis, and the reliability of the proposed analytical method was confirmed.
We investigated the daily intake of boron through the total diet study method from 1992 to 1997 by use of the proposed ICP-atomic emission spectrometry. The daily intake of boron from 1992 through 1997 was 1.23-2.15mg in Osaka district.View full abstractDownload PDF (713K) -
Akihiko TAKINO, Taizo TSUDA, Mihoko KOJIMA, Hiroyuki HARADA, Kazue MUR ...1999 Volume 40 Issue 4 Pages 325-333_1
Published: August 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn analytical method was developed for bisphenol A (BPA) in canned fish and meat. A sample was extracted with 150mL of ethyl acetate and the organic layer was washed with 150mL of 5% NaCl solution. After defatting by n-hexane/acetonitrile partition, the extract was loaded on a 10% water-dehydrated silica gel column, washed with 100mL of 15% acetone/n-hexane and eluted with 50mL of 60% acetone/n-hexane. The eluate was evaporated to dryness and dissolved in methanol. BPA was analyzed by HPLC [column, Wakosil 5C18 (4.6mm i. d.×150mm); mobile phase, 60% methanol; detection, 230nm; flow rate, 0.8mL/min].
Recovery of BPA in 20g of canned tuna steak (maguro steak) fortified with 1μg of BPA was 89.9±7.1 (n=3). Four kinds of canned fish and three kinds of canned meat on the market were analyzed for BPA with the developed method. The ranges of BPA content were N. D. -319ng/g. The detection limit of BPA was 25ng/g.
This method was effective for BPA in canned fish and meat and does not use solvents such as chloroform and dichloromethane.View full abstractDownload PDF (838K)
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