The metabolism of
14C-aflatoxin B
1 (Af. B
1) in the chick embryo was studied.
When inoculated into air cells, the embryos, egg membranes, other parts of the eggs and the expired carbon dioxide during a 1 hour period contained 8.0, 15.0, 76.0 and 1.0% of the total detected radio-activity, respectively. In the case of yolk sac inoculation, the embryos, other parts of the eggs and the expired carbon dioxide during a 1 hour period contained 3.4, 96.4 and 0.2% of the total detected counts, respectively.
At equal doses of (
14C)-Af. B
1 into the air cell and yolk sac of eggs, the embryos incorporated
14C in a ratio of 2.5:1, which is similar to the ratio of LD
50 values (air cell inoculation=0.41μg/egg; yolk sac inoculation=0.89μg/egg) by the two inoculation routes.
The homogenate of embryos inoculated with Af. B
1 was partitioned into chloroform and methanol-water. As the time after inoculation increased, methanol-water-soluble metabolites from Af. B
1 increased and chloroform-soluble ones decreased. Af. M
1 was the principal metabolite among the chloroform-soluble substances.
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