All issues
Volume 14 (1973)
- Issue 6 Pages 501-
- Issue 5 Pages 403-
- Issue 4 Pages 343-
- Issue 3 Pages 219-
- Issue 2 Pages 137-
- Issue 1 Pages 1-
Volume 14, Issue 2
Displaying 1-11 of 11 articles from this issue
- |<
- <
- 1
- >
- >|
-
Kaoru KATSUMURA, Mutsuo ISHIZAKI, Akiko SASAMOTO, Tokihiro SASAMOTO, S ...1973 Volume 14 Issue 2 Pages 137-141
Published: April 05, 1973
Released on J-STAGE: March 01, 2010
JOURNAL FREE ACCESSA new simplified method for microdetermination of mercury in fish is described.
In this method, the two necked oxygen-flask with two necks of 1000ml capacity was used for burning the biological material. Before the sample was burnt in the oxygen-flask, the oxygen-flask was evacuated with a vacuum pump, then the oxygen-flask was filled with oxygen. These operations were repeated three times, and at the end of these operations the internal pressure of the oxygen-flask was adjusted to about 610mm Hg for avoidance of explosion of the oxygen flask and loss of mercury.
After the sample was burnt, the oxygen-flask was cooled with a dry ice-acetone mixture to condense the vaporized mercury. Then the mercury and some vaporized components were absorbed to 0.1N sulfuric acid. The volatile components except mercury in the absorbant solution were extracted with n-hexane, the water layer being used as the prepared solution.
The mercury in the prepared solution was determined by flameless atomic absorption spectrometry. As a result of repeating this method on a few kinds of fish, it was found that the mercury in fish sample could be determined with 100-105% of recovery ratio and about 5% of variation coefficient.View full abstractDownload PDF (813K) -
Effect of Cooking on Removal of Organochlorine Pesticide Residues from Rice, Red Beans and Soy BeansMitsuharu TAKEDA, Kumiko OTSUKI, Hiroshi SEKITA, Hiroya TANABE, Sachik ...1973 Volume 14 Issue 2 Pages 142-148
Published: April 05, 1973
Released on J-STAGE: March 01, 2010
JOURNAL FREE ACCESSEffect of cooking, especially boiling, on the removal of organochlorine pesticide residues from polished rice, red beans and soybeans were examined.
The result shows that a little part of pesticide residues was removed from polished rice and red beans, while considerable amount of pesticide residues was removed from soybeans by boiling. 31% of α-BHC, 43% of β-BHC, 67% of γ-BHC, 20% of pp′-DDE and 80% of pp′-DDT were eliminated in the cooking process of soybeans.View full abstractDownload PDF (1478K) -
I. Application of Batch Operation on CM-Sephadex Ion ExchangerYoshiaki HARADA1973 Volume 14 Issue 2 Pages 149-159
Published: April 05, 1973
Released on J-STAGE: July 27, 2010
JOURNAL FREE ACCESSProcedures were developed for assay of staphylococcal enterotoxins in foods, using microslide gel double diffusion test.
(1) Foods were prepared for assay by addition of water (homogenization or mashing in case of cereal foods) adjustment of pH to 7.4-7.6, blending, and centrifugation. The supernatant fluids were then adjusted to pH 4.5 (in case of animal foods) or pH 6.0, and centrifuged. Supernatant fluids taken at pH 4.5 were readjusted to pH 6.0, and centrifuged. From these supernatant fluids, enterotoxins were partially purified by adsorption on CM-Sephadex C-50 equilibrated with 0.01M sodium phosphate (pH 6.0), washing with 0.01M sodium phosphate (pH 6.0), then eluted with 0.2M sodium chloride in 0.1M sodium phosphate (pH 7.6), and concentrated by dialysis against polyethylene glycol.
Adequate dosage levels of CM-Sephadex in the batch ion exchange for various foods were determined, individually.
(2) By these procedures, enterotoxins were detected in 12 out of 16 foods suspected for cause of poisoning outbreaks. Enterotoxin A (50%) and A+D (33%) were predominant types. Concentrations of enterotoxins detected in foods were ranging up to 0.3μg/g, and estimated at 0.05μg/g on the average. In 4 outbreaks, neither enterotoxins nor enterotoxin-producing staphylococci were isolated from the suspected foods.
(3) The extract from suspected cream puff containg 4.5μg of enterotoxin A induced emetic responses in a volunteer by feeding test.View full abstractDownload PDF (2018K) -
Manabu SUZUKI, Yasuhiro YAMATO, Tadao WATANABE1973 Volume 14 Issue 2 Pages 160-167
Published: April 05, 1973
Released on J-STAGE: March 01, 2010
JOURNAL FREE ACCESSTranslocation of residual pesticides in soil was investigated and following results were obtained.
i) Each residual BHC isomer in soil was more translocated into carrots, turnip foliages, radish foliages and spinachs than tomatoes and cabbages. From the pesticide residue levels in soil, there are possibilities to exceed the pesticide tolerances in carrots and turnip foliages.
ii) The translocation ratios of aldrin and dieldrin into cucumbers, carrots and spinachs were 16.6%, 9.6% and 4.4%, respectively. And aldrin was detected in the form of dieldrin in vegetables. The translocation ratio into cucumbers was almost the same as the results previously published, however, the ratios of radishes and carrots were lower.
iii) The translocation ratios of endrin into cucumbers, cabbages, and radishes were 21.8%, 14.5% and 9.1%, respectively. Judging from translocation ratios of endrin, aldrin anh dieldrin into vegetables and pesticide residue levels in the soil, there are much possibilities that the residue levels in some vegetables might exceed the pesticide tolerance in Japan.
iv) Althogh DDT, its isomer and metabolites remained in relatively high residue levels in the soil, little translocation into vegetables being observed.View full abstractDownload PDF (1200K) -
Hideo HASHIMOTO, Yukio MURAKAMI, Yoshiyuki OHTA1973 Volume 14 Issue 2 Pages 168-172
Published: April 05, 1973
Released on J-STAGE: July 27, 2010
JOURNAL FREE ACCESSSeventy-five samples of commercial sliced ham were examined on the contamination of psychrotrophic bacteria. The strains isolated from those samples were subjected to investigation of the optimal growth temperature and the enzymatic activities at different temperatures.
It was found that both the total and the psychrotrophic bacterial counts were under 103per gram in most of the samples examined.
The growth degree of the isolates was examined at 5, 15, 25, 30 and 37°C, and more than 90% of the strains tested showed the best growth between 15 and 30°C.
Enzymatic activity of the isolates was examined at 25 and 5°C. At 25°C, production of lipase, protease and amylase was observed for 294 (70.6%), 93 (22.3%) and 19 strains (4.5%), respectively. On the contrary, at 5°C, 298 (71.6%), 231 (55.5%) and 5 strains (1.2%) produced lipase, protease and amylase, respectively. Protease-positive strains were predominant, especially, at low temperature.View full abstractDownload PDF (964K) -
2) 3, 4-Benzopyrene in Japanese Daily FoodsYoshiko SHIRAISHI, Tsuyako SHIROTORI, Eigo TAKABATAKE1973 Volume 14 Issue 2 Pages 173-178
Published: April 05, 1973
Released on J-STAGE: March 01, 2010
JOURNAL FREE ACCESSLittle has been reported on 3, 4-benzopyrene in Japanese foods, but it is requested to investigate the content of this carcinogenic polycyclic hydrocarbon in our daily foods in view of the high incidence of gastric cancer in this country.
The present paper deals with the further improvement made on the previously reported analytical procedure for 3, 4-benzopyrene in Japanese teas and the application of the improved method to 18 kinds of daily foods including rice, “Miso” (bean paste), and soy sauce. The recoveries of 3, 4-benzopyrene added to each 100g of “Katsuobushi” (dried bonito) and “Nori” (purple laver) at a level of 20ppb averaged 64% and 90%, respectively. No 3, 4-benzopyrene was detected in rice, “Miso”, soy sauce and soybean which are eaten by Japanese almost everyday. On the contrary, this carcinogen was detected in all the samples of dried bonito “Katsuobushi” and sea weeds such as laver “Nori”, “Wakame” (Undaria pinnatifida), etc. in fairly large quantities; 29.8ppb in “Katsuobushi” and 31.3ppb in “Nori” at the maximum.
It was confirmed that the improved method is applicable for analyzing 3, 4-benzopyrene in many kinds of foods.View full abstractDownload PDF (888K) -
Studies on Analysis of Thiabendazole in Citrus FruitsMidori MIHARA, Tatsuo KONDO, Hiroya TANABE1973 Volume 14 Issue 2 Pages 179-185
Published: April 05, 1973
Released on J-STAGE: March 01, 2010
JOURNAL FREE ACCESSSeveral methods for the determination of thiabendazole in citrus fruits were studied.
Thiabendazole was extracted with ethyl acetate and re-extracted from the organic phase with 0.1 N-HCl. The acidic extract was made alkaline, extracted with ethyl acetate, and the organic phase was re-extracted with 0.1 N-HCl. When this procedure for extraction was not carried out, the determination was interfered with unknown substance A which had an absorption maximum at 283mμ.
When its concentration was more than 1ppm, the test solution was spectrophotometrically measured at 302mμ, and when its concentration was less than 1ppm, the test solution was spectrofluorometrically measured with an excitation wavelength at 305mμ and an emission wavelength at 350mμ.
In the gas chromatographic method, it was detected with DC-200 and QF-1 at 200°C. Since its sensitivity was very weak, this method was not practical.
Thiabendazole in imported citrus fruits, grapefruits, oranges and lemons, were determined by spectrofluorometry. However, they contained unknown substance B which gave a fluorescence spectrum with an excitation maximum at 330mμ and an emission maximum at 400mμ. Fortunately, since its concentration was low, this method would be successfully applied.View full abstractDownload PDF (981K) -
Hironoshin KAWASAKI, Tadashi NAGATA, Seizaburo KANOH1973 Volume 14 Issue 2 Pages 186-190
Published: April 05, 1973
Released on J-STAGE: March 01, 2010
JOURNAL FREE ACCESSWe studied on the toxicity of tetrodotoxin of Fugu which was imported to Japan, by use of mice and the following results were obtained:
1) Crystalline tetrodotoxin exhibited the strongest toxicity in mice by intracerebral injection. The degree of toxicity by administration routes was expressed by the following order: intracerebral, intravenous, intraperitoneal, subcutaneous and oral.
2) The dose of toxin corresponded to the death time.
3) The toxicity of tetrodotoxin was decreased by the addition of tissue homogenate of Fugu (tetrodon).
4) Nashi-Fugu, and Nagoya-Fugu were not so toxicic as other Fugs.View full abstractDownload PDF (782K) -
Teruo FUKUDA, Reiko KANEDA, Takako INOUE1973 Volume 14 Issue 2 Pages 191-195
Published: April 05, 1973
Released on J-STAGE: March 01, 2010
JOURNAL FREE ACCESSA paper electrophoretic method for identification of nucleotides (AMP, ADP, ATP, GMP, GTP, CMT, CTP, TMP, UMP, UTP, IMP), nucleosides (adenosine, guanosine, cytidine, thymidine, uridine, inosine), and nucleic bases (adenine, guanine, hypoxanthine, cytosine, thymine, uracil) was investigated by using 5N acetic acid, 5N formic acid, 1N citric acid, M/15 disodium orthophosphate solution and 1% borax solution as the electrolyte. The relative mobilities of the compounds were expressed against flavianic acid or auramine. It was found that all compounds, investigated in this study, could be separated and identified by systematic use, in order, of the above-mentioned electrolyte.View full abstractDownload PDF (815K) -
Yukio TANAKA, Katsuhiko IKEBE, Ryoichi TANAKA, Nobuharu KUNITA1973 Volume 14 Issue 2 Pages 196-201
Published: April 05, 1973
Released on J-STAGE: March 01, 2010
JOURNAL FREE ACCESSDownload PDF (1132K) -
1973 Volume 14 Issue 2 Pages 216
Published: 1973
Released on J-STAGE: March 01, 2010
JOURNAL FREE ACCESSDownload PDF (36K)
- |<
- <
- 1
- >
- >|