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In the previous work by Yasunaga and Zenitani, the halophilic bacteria belonging to Miyamoto's so-called euryhaline type were obtained in a great number from river water not mixed with sea water in Nagasaki City, and it was shown that some of the isolates in summer were almost similar in biological characters to so-called pathogenic halophilic bacteria, being considered to be responsible for the most of food poisonings caused mainly by fishes and shells.
The present work was undertaken to ascertain the existence of the organisms in city river water, presumed from the previous results. The characters of the river waters collected as samples for detecting the organisms are shown in Table 1.
The organisms were isolated from the samples by using a peptone water with 3% NaCl as final concentration and a nutrient agar medium with 3% NaCl, 0.1%, bile salts, sucrose and BTB. The incubations were performed at 37°C for 20-24 hours. The rate of isolation of the strains identified as the pathogenic halophilic bacteria, the classification of their biotypes by V.P. test and salt requirment, and grouping of O serotypes are presented in Tables 2, 3 and 4.
From these results, it was confirmed that upper stream water than estuary in Nagasaki City were considerably contaminated with the pathogenic halophilic bacteria in summer, and this may explain to some extent the predominant distribution of the organisms in coastal area, especially in estuary. Furthermore, the facts that 10 of 24 strains isolated were belonging to biotype 1, and that the majority of them were classified into O1-O5 groups suggest possibility of the infection by the organisms through city river water.

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In Japan, the presence of tin in refreshing beverages is permitted at the concentration of not exceeding 150ppm. Fruit juices and fruits in lacquer-lined cans generally contain tin ranging from 10ppm to 50ppm. In these cases, a colorimetric method is preferable to a gravimetric method for the determination of tin content. Phenylfluorone is generally used as a reagent to determine tin colorimetrically, but, according to the conventional colorimetric method, tin is determined in the presence of this excessive reagent and the absorbance of the test solution shows usually 0.15-0.25 at about 510mμ.
The authors have improved the sensitivity and precision of this method by making tin in the test solution react with phenylfluorone, extracting the excess of phenylfluorone with chloroform, and reducing the absorbance of blank solution below to 0.1.

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Previous report (Aiso et al, 1962) dealt with macroscopic examination of rats fed 2- (2-furyl) -3- (5-nitro-2-furyl) acryl amide and nitrofurazone. In the present study we have observed their liver, kindney, heart, spleen and testis histologically.
Degenerative changes, atrophy, hydropic degeneration, pyknosis, and fatty metamorphosis of the hepatic cells, were observed in all groups of the rats fed nitrofurazone, but the extend of disorders was mild in general, and the degree of changes was more intensive when the rate of chemical concentration in the diet was increased. Cirrhotic changes were not recognized.
On th contrary, the tendency of hypertrophy of the hepatic cells was noted in the rats fed 2- (2-furyl) -3- (5-nitro-2-furyl) acryl amide, especially at the 0.02% and 0.2% level. Degenerative changes were markedly observed in only a group of rats fed the diet at the level of 0.2% for one year, and their growth was retarded. Cirrhotic changes were also not recognized.
There was nothing of the neoplastic changes in hepatic cells of all the rats fed both derivatives.
Remarkable toxic effect in the kidney, heart, spleen and testis was not observed in all groups fed both compounds.

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Seven different samples of shortening and five different samples of lard were kept at 5°C and 30°C during twelve months to examine degradation of these samples and acid value and peroxide value of these samples were estimated every month in the course of the examination.
As a result, lard kept at 5°C and 30°C underwent little change and was far more stable than margarine, but one of five samples of lard which was kept at 30°C, extremely degraded. After finishing the examination, melting point, saponification value, iodine value, thiocyanogen value and colour of these samples were estimated, but any changes due to the difference of storage temperatures were not observed.

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5-40×10^-5M Solutions of 2, 4, 5- and 2, 4, 6-trichlorophenol, and 2, 4-, 2, 5- and 2, 6-dichlorophenol can be accurately determined by the absorbance at the isosbestic points of the longest wavelength, especially in 0.01-0.1M hydrochloric acid and 0.5-2% ethanol. Because at the condition, the isosbestic points are situated at near the maxima of the spectra.
And pK' of chlorophenols are determined spectrophotometrically. Some solvent effect of ethanol and water upon the spectra of chlorophenols are examined.

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It was found that the addition of methyl bromide to ethylene oxide, generally had effect of narrow down the limits of explosivity. This non-explosive mixture retained very well its effect as a fungicide and insecticide.

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There was neglegibie lowing of the strength of α-amylase activity throughout the experiment of fumigation, as recorded by chimotachigraph, amylograph and albeograph.
There was no degrade in the amount of glutein or in the β-amylase activity. By the above treatment, there was slight degradation in the special flavour and taste of the baked products, but it was concluded that there was not too much disadvantage practically.

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The results indicate that propylene oxide is inferior to ethylene oxide as a fungicide. It has no superiority when compared with methyl bromide and chloropicrin, which have the advantage of non-explosive.
These results show that the reagent has limited use as a fungicide.

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The action of enzymes such as diastase, takadiastase, pancreatin, amylase of Bacillus subutilis, cellulase and saliva, on carboxymethylcellulose (CMC), carboxymethylstarch (CMS), and methylcellulose (MC), was studied concerning the production of reducing sugar and the change of the viscosity of each 1% aqueous solution.
Cellulase produced some reducing sugar from CMC and reduced the viscosity of CMC aqueous solution, and takadiastase followed it. Pancreatin, amylase of B. subtilis and saliva produced little or no reducing sugar from CMC, but these enzymes produced reducing sugar from CMS and reduced the viscosity of CMS aqueous solution. MC was affected slightly by cellulose but other enzymes gave no influence on MC.

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The authors have analysed the correlation between the dietary intake of nutrition and hematocrit, hemoglobin, erythrocytes, specific gravity of blood, physical and economic status. Studies were carried out on the total number of 72 men and women who work in medium and small enterprises in Tokyo.
The results were as follows:
1. The dietary intake of calories, vitamin A, vitamin B₂, calcium and iron were deficient about 20-55% compared with the amount of their nutritional requirements.
2. Body height, weight and weight-height ratio were lower than the average of in this country in males and females.
3. There were no correration between the animal protein intake and weight-hight ratio.
4. The coeficient correlation between the animal protein intake per day and hemoglobin and hematocrit values in blood were r=0.367 r=0.343 in males, these were significant at the 0.05 level.
5. The coeficient of correlation between the animal protein intake per day and the income per month were r=0.417 in males, r=0.341 in females, these were significant at the 0.05 level.

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The authors had studied on the usefulness of cow's milk fermented by some kind of “Family Lactbacillaceae” as a drink for health.
The results were as follows:
1. In the group which drank every day for five months their body weight increased cleary in comparison with the nondrinking group.
2. It was recognized that their hemoglobin values in blood trended to increase in the drinking group similarly.
3. In the survey of subjective symptoms by a health calendar, 80per cent of drinkers, who continued drinking cow's milk fermented by some kind of “Family Lactbacillaceae”, recognized some effectiveness for health.

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