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Masamichi TOYOMIZU, Yukio TOMIYASU
1963 Volume 4 Issue 1 Pages
1-9
Published: 1963
Released on J-STAGE: November 22, 2010
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Tatsumi AOKI, Masayoshi NAKAHARA, Takashi KASHIMOTO
1963 Volume 4 Issue 1 Pages
10-14
Published: 1963
Released on J-STAGE: November 22, 2010
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The authors investigated germ-inhibitory substances in marketed milk taken from various suburban parts of Osaka from March to October, 1962, the test method for the substances was based on a modification of Resazurin test published by T. Matsui, as well as on a biochemical method.
The results were summerized as follows;
1) Samples from 26 milk plants were subjected to Resazurin test, 8 among the samples were found positive to Resazurin test.
2) NFA was detected in the samples from 7 plants by the absorption spectral analysis as well as by the bioutographic analysis on their n-butanol extracts.
3) 72% of 25 strains isolated from the samples in which NFA was detected showed a resistance to NFA, 60% to Penicillin and O% to Streptomycin.
Judging from the results of our investigations it seems to us that the strict inspection of milk is needed for public health.
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Kunio OKAHARA, Yoshio KURAYUKI, Motohachi ISEKI, Shigeru TANIGUCHI, Ak ...
1963 Volume 4 Issue 1 Pages
15-31
Published: 1963
Released on J-STAGE: November 22, 2010
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Pharmacological actions of alkylbenzenesulfonate (ABS) are determined. The results are as follows:
1. LD
50's (24hr) of 98.7% ABS for mice are 1, 407mg/kg (or.), 1, 989mg/kg (sc.), and 157 mg/kg (iv.). That of 54.8% ABS is 2658mg/kg (or.).
2. After a single ABS injection of 5mg/kg in rabbits the respiration is accelerated, but the blood pressure is depressed.
3. 10
-5ABS solution decreases myocardial contraction in isolated hearts of toads.
4. Isolated blood vessels of rabbit ears are dilated by ABS in the concentration of 10
-5, but constricted in that of 10
-4. 5. ABS causes the abnormality of peristalsis in isolated rat uterus at concentration of 10
-5.
6. Hemolysis of rabbit blood occurs in 80-90 ppm ABS solution.
7. When 20% ABS in a dose of 0.15ml/kg/day is administered to rats for 6 months, the growth rate shows no differences between control and test group. On gross examination, spleens of test group rats are slightly smaller than that of control group ones.
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1. Relation among Heat Resistance, Release of Dlpicollnlc Acid, and Increase of Stainability
Hachiro MURAKAMI
1963 Volume 4 Issue 1 Pages
32-35
Published: 1963
Released on J-STAGE: November 22, 2010
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Spores of two strains of Bacillus subtilis, Bac. subtilis PCI 219 (PCI 219) and Bac. subtilis TR 33 (TR 33, a laboratory strain) were studied on their heat resistance, release of dipicolinic acid and in creasein stainability caused by heating or germination.
(1) TR 33 was much higher resistant to heat than PCI 219; three hours of heating at 100°C were required for TR 33 to reduce its germination rate to 17 per cent, while PCI 219 required only 20 minutes to fall to fall to the equall level.
(2) Heat was more effective on PCI 219 than on TR 33 in order to induce release of dipicolinic acid and to increase stainability of the spores; by heating at 100°C for 20 minutes with PCI 219 and far 4 hours with TR 33, 90 per cent of dipicolinic acid was equally produced from each of the spores, and regarding increase in stainability up to 50 per cent, TR 33 required heating for more than 4 hrs. but PCI 219 heating only for 20 minutes.
(3) Increase in total stainability caused both by heating and by germination of the heated spares appeared not to follow a pattern similar to that of the release of dipicolinic acid from the spores treated in the same way.
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Simultaneous Determination of Degrees of Adsorption and Growth-Inhibition
Chikataro KAWASAKI, Yoshiro NAMBA, Kozo OKADA, Hiromitsu YAMADA
1963 Volume 4 Issue 1 Pages
36-39
Published: 1963
Released on J-STAGE: November 22, 2010
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An experimental procedure was investigated to simultaneously determine the amounts of adsorbed cationic surface-active agents on baker's yeast as well as, growth-inhibition of the yeast, and the following one was proved to be satisfactory: A centrifugetube containing a suspension of 10mg (in wet weight) baker's yeast in 4 ml phosphate-buffer of pH 7 with an addition of one ml of a surface-active agent: solution each in different concentration, is incubated for 10 minutes at room temperature and then centrifuged. The precipitated yeast-cells are twice washed with each 5 ml of sterilized water and finally are made up to 10 ml with water. One drop of the yeast-suspension is inoculated into 10 ml of a Reader broth and the growth is measured turbidometrically by absorbancy at 610 mμ after the incubation for 180 or 42 hours. The surface-active agent in the supernatant and washed water which has been separated from the yeast-cells, is determined colorimetrically by the eosine-chloroform-extraction method.
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Correlation between Adsorption of surface-Active Agents on Yeast-Cells and Growth-lnhibition of the Yeast
Chikataro KAWASAKI, Yoshiro NAMBA, kozo OKADA, Hiromitsu YAMADA
1963 Volume 4 Issue 1 Pages
39-43
Published: 1963
Released on J-STAGE: November 22, 2010
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Adsorption of germicidal quarternary ammonium salts on yeast-cells and their effects to growth of the yeast were simultaneously observed by the authors' method.
1) Lauryldimethyl-β-hydroxyethylammonium salts which consist of different kinds of anions, were shown to have no significant difference among the compounds both in their adsorption and growth-inhibition.
2) Both a) alkyldimethylbenzylammonium salts and b) alkyldimethyl-γ-chloro-β-hydroxypropylammonium salts indicated the tendency of lncreasing adsorption on yeast-cells in accordance with the increase of alkyl chain-length from lauryl to stearyl. Growth-inhibition of the yeast, by a) type compounds, was proved to be approximately proportional to the chain-length, whereas the growth-inhibition by b) type compounds was shown to be contrary to the chain-length.
3) Among 5 kinds of lauryldimethylammonium chlorides which contain benzyl or alkyl radicals, γ-chloro-β-hydroxypropyl compound was proved to be the highest both in adsorption on yeast-cells and growth-inhibition. The growth-inhibition of yeast was observed by other compounds in a descending order of benzyl, β-hydroxyethyl, methyl and β-hydroxypropyl radicals.
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Identification of Malachite Green and Brilliant Green
Toshio TERASHIMA
1963 Volume 4 Issue 1 Pages
43-48
Published: 1963
Released on J-STAGE: November 22, 2010
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High-voltage paper electrophoresis was carried out for the identification of Malachite Green and Brilliant Green using 5N AcOH (10% propylen glycol sol.) as an electrolyte.
Both of them migrated towards cathode, forming green bands respectively.
In the case of Brilliant Green only, however, another green band appeared shortly after evaporation of the solvent from the paper.
As a result of my examination, the shorter migrated fraction of these two bands of Brilliant Green was graved to be singly charged ian and the longer one was doubly charged colorless salt.
The lack of this phenomenon in Malachite Green seems to be caused by the difference existing between the basicities of these two dyes.
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Masahiko FUJITA
1963 Volume 4 Issue 1 Pages
53-60
Published: 1963
Released on J-STAGE: November 22, 2010
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Akio ISHII
1963 Volume 4 Issue 1 Pages
61-67
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68a
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68b
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68c
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68d
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68e
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68f
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68g
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68h
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68i
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68j
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68k
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68l
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68m
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68n
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68o
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68p
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68q
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
68r
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69a
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69b
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69c
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69d
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69e
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69f
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69g
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69h
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69i
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69j
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69k
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69l
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69m
Published: 1963
Released on J-STAGE: November 22, 2010
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1963 Volume 4 Issue 1 Pages
69n
Published: 1963
Released on J-STAGE: November 22, 2010
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