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Volume 40 (1999)
- Issue 6 Pages 417-
- Issue 5 Pages 335-
- Issue 4 Pages 259-
- Issue 3 Pages 183-
- Issue 2 Pages 123-
- Issue 1 Pages 1-
Volume 40, Issue 2
Displaying 1-18 of 18 articles from this issue
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Small Round Structured VirusHiromasa SEKINE1999 Volume 40 Issue 2 Pages 123-130
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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Masao GOTO, Mamoru TOTSUKA, Shuichi KAMINOGAWA1999 Volume 40 Issue 2 Pages 131-136
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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Yasuo TANAKA, Tadayoshi SASAO, Tadashi KIRIGAYA, Shiro HOSOI, Taro KAW ...1999 Volume 40 Issue 2 Pages 137-142_1
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSTwo unknown peaks were detected on the chromatogram of turnip greens extract by GC-FPD. These unknown peaks were identified as dimetonthiol sulfone and disulfoton sulfone, which are metabolites of disulfoton, by GC/MS. As a result of investigation of 300 samples of crops, dimetonthiol sulfone was detected in the range of 0.22-1.6μg/g and disulfoton sulfone was detected in the range of 0.002-0.54μg/g in turnip, turnip greens and cauliflower. The recoveries of disulfoton and its metabolites fortified at the levels of 1.0μg/g and 10.0μg/g were more than 90%. The detection limit of dimetonthiol sulfone was 0.01μg/g and those of disulfoton and disulfoton sulfone were 0.001μg/g.View full abstractDownload PDF (570K) -
Toshio HIDAKA, Tadashi KIRIGAYA, Taro KAWAMURA, Saju KAWAUCHI1999 Volume 40 Issue 2 Pages 143-148_1
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSGel filtration of cabbage treated with sodium hypochlorite (NaClO) affords a fraction showing a high level of chloroform (CHCl3), and this was studied to identify the precursors of CHCl3.
The mass spectral ion peaks of the fraction obtained by LC/MS suggested the presence of five kinds of amino acids. Furthermore, analysis of amino acids by HPLC revealed eight kinds of amino acids including those detected in LC/MS. The concentrations were 48.3μg/mL for L-glutamine and less than 10μg/mL for other amino acids. An amino acid standard solution prepared with amounts similar to those in the fraction was treated with NaClO for 24 hours. The consequent CHCl3 formation was 82.0% of that formed from the fraction. In particular, L-alanine contributed a great deal to the formation of CHCl3, while L-glutamine formed little CHCl3. Thus, L-alanne constituents identified in the gel filtration fraction are considered to be the major precursors of CHCl3 formed in cabbage by NaClO treatment. This result is different from the previously reported findings on CHCl3 precursors of soybean sprouts.View full abstractDownload PDF (743K) -
Takeshi MATSUOKA, Yoshimi KAWASHIMA, Hiroshi AKIYAMA, Hirohito MIURA, ...1999 Volume 40 Issue 2 Pages 149-157_1
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method using polymerase chain reaction (PCR) was designed for the detection of food or food ingredients derived from genetically modified soybeans (GMS), imported from the United States, in a mixture with conventional non-genetically modified soybeans (non-GMS). The presence of recombinant deoxyribonucleic acid (DNA) in the soybeans could be detected with three different pairs of specific oligonucleotide primers designed from the sequences of the introduced genes. The soybean intrinsic lectin Le1 gene was used as an internal control. The results of the PCR amplification indicated that a method using cetyltrimethylammonium bromide (CTAB) was most suitable for DNA extraction from soybeans and the processed foods. The recombinant DNA could be detected in dry soybeans containing 0.05% GMS and tofu made from soybeans containing 0.5% GMS. Of 41 commercial tofu samples, recombinant DNA was detected from 27 tofu samples. It is, however, difficult to carry out PCR on DNA extracted from soybeans steamed at 131°C or on fermented natto, although the Le1 gene was detected from soybeans steamed at 115°C and in the fermented natto when a nested PCR technique was employed.View full abstractDownload PDF (9530K) -
Yoko KAWAMURA, Hiromi SANO, Takashi YAMADA1999 Volume 40 Issue 2 Pages 158-165_1
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSBisphenol A (4, 4′-isopropylidene diphenol) in drinks was extracted with a polystyrene solid-phase cartridge, trimethylsilylized, then determined by GC/MS/SIM. Forty-seven commercial canned drinks were surveyed. The bisphenol A concentration and detection frequency in coffee, black tea and other tea drinks were 3.3-213ng/mL (11/13), 8.5-90ng/mL (4/9) and 3.7-22ng/mL (5/8), respectively. In alcoholic drinks bisphenol A was detected in only one sample (13ng/mL, 1/10) and none was detected in 7 soft drinks. As regards can coating materials, polyvinyl chloride coating of the lid might cause high migration of bishphenol A, followed by epoxy resin coating of both the lid and body, while epoxy resin coating of the lid and PET coating of the body caused only low migration. During the storage of canned drinks at 60°C, bisphenol A concentration was unchanged. The highest migration level of bisphenol A in a can was 40μg in this test.View full abstractDownload PDF (995K) -
Chigusa KOBAYASHI, Mitsuo NAKAZATO, Hirofumi USHIYAMA, Yuka KAWAI, Yuk ...1999 Volume 40 Issue 2 Pages 166-171_1
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple method for the simultaneous determination of five artificial sweeteners, alitame (AL), acesulfame K (AK), saccharin (SA), aspartame (APM) and dulcin (DU) in various foods by high performance liquid chromatography (HPLC) was developed.
Chopped or homogenized samples were packed into cellulose tubing with 0.01mol/L hydrochloric acid containing 10% sodium chloride, and dialyzed against 0.01mol/L hydrochloric acid for 24-48 hours. Tetra-n-butylammonium bromide and pH 5.0 phosphate buffer were added to the dialyzate. The solution was passed through a Sep-Pak Vac C18 cartridge, and the cartridge was washed with water and a mixture of methanol-water (1:9). The five sweeteners were eluted from the cartridge with a mixture of methanol-water (45:55). The sweeteners were separated on an Inertsil ODS-2 column with a mobile phase of methanol-water (1:3) containing 0.01mol/L tetra-n-propylammonium hydroxide adjusted to pH 3.5 with phosphoric acid and were detected at 210nm.
The recoveries of the five sweeteners from various kinds of foods spiked at 200μg/g ranged from 77-102%. The detection limits of the five sweeteners were 10μg/g in the samples.View full abstractDownload PDF (702K) -
Yuiko TAKEDA, Yoko KAWAMURA, Takashi YAMADA1999 Volume 40 Issue 2 Pages 172-177_1
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSMigration of aluminium from disposable aluminium foil pans and trays into foods was investigated. When a sample was cooked with soup for noodles (tsuyu) previously diluted according to the directions, the migration level of aluminium from the pan was from 1/2 to 1/6 of that when tap water was first boiled in the pan and then tsuyu and foods were added. That is, the migration of aluminium was depressed by the addition of tsuyu and/or foods.
The migration level of aluminium into commercial dishes cooked in a disposable aluminium foil pan by boiling according to the directions was less than 0.2μg/g. In the case of commercial dishes cooked in a disposable aluminium foil tray by baking, the migration level was not more than 0.03μg/g.
The migration of aluminium from disposable aluminium foil vessels into foods was less than that into food-simulating solvents, so the data suggest that the migration was depressed by adding foods.
These results suggested that the human intake of aluminium from disposable aluminium foil vessels is extremely low.View full abstractDownload PDF (719K) -
Takeshi KOBAYASHI, Bon KIMURA, Tateo FUJII1999 Volume 40 Issue 2 Pages 178-182_1
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThis study was designed to clarify the mechanism of the decline in the toxicity of fermented puffer fish ovaries manufactured and consumed as a local dish in the coastal area of mid-northern Japan.
Six ovaries were obtained from a factory processing fermented puffer fish ovaries. These ovaries were divided into two portions: the unsterilized ovaries and the ovaries sterilized by autoclaving. Then, tetrodotoxin was added to these ovaries and they were stored at 25°C for 24 weeks in the laboratory. During storage, the changes of toxicity were compared by mouse bioassay. The toxicity of both tetrodotoxin-unsterilized ovary mixtures and tetrodotoxin-sterilized ovary mixtures decreased. For example, the initial toxicity of 51MU/g of one of the tetrodotoxin-unsterilized ovary mixtures decreased to 25MU/g, and that of 41MU/g of one of the tetrodotoxin-sterilized ovary mixtures decreased to 25MU/g after 24 weeks. These results suggest that the decrease of toxicity during storage does not involve microbial metabolism.View full abstractDownload PDF (546K) -
[in Japanese]1999 Volume 40 Issue 2 Pages J222-J223
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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[in Japanese]1999 Volume 40 Issue 2 Pages J223-J224
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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[in Japanese]1999 Volume 40 Issue 2 Pages J224-J226
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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[in Japanese]1999 Volume 40 Issue 2 Pages J226
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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[in Japanese]1999 Volume 40 Issue 2 Pages J227
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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[in Japanese]1999 Volume 40 Issue 2 Pages J227a-J228
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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[in Japanese], [in Japanese]1999 Volume 40 Issue 2 Pages J229-J230
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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[in Japanese]1999 Volume 40 Issue 2 Pages J230-J231
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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[in Japanese]1999 Volume 40 Issue 2 Pages J231-J233
Published: April 05, 1999
Released on J-STAGE: December 11, 2009
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