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Volume 38 (1997)
- Issue 6 Pages 391-
- Issue 5 Pages 275-
- Issue 4 Pages 193-
- Issue 3 Pages 117-
- Issue 2 Pages 39-
- Issue 1 Pages 1-
Volume 38, Issue 4
Displaying 1-11 of 11 articles from this issue
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Zenro IKEZAWA1997 Volume 38 Issue 4 Pages 193-203
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (2071K) -
Takashi OGAWA, Naoko OKAMOTO, Katsuhiko TANIGUCHI, Kenshi YAMASHITA, S ...1997 Volume 38 Issue 4 Pages 204-210_1
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSWhen acephate and methamidophos in agricultural products were determined by GC-FPD, the recoveries were more than 100%. We therefore prepared a standard solution for analysis of the pesticides by dissolving acephate and methamidophos in yeast extract solution, and used it as a calibration standard. The detection limits of acephate and methamidophos were 0.01ppm for agricultural products. Recoveries of acephate and methamidophos added to agricultural products at 0.1ppm and 0.05ppm were 97-99% and 80-92%, respectively. The present method was applied to the analysis of 447 agricultural products. Acephate and methamidophos were detected in 12 samples. The levels of acephate residues in 10 samples were 0.03-1.2ppm and the levels of methamidophos residues in 7 samples were 0.01-4.3ppm.View full abstractDownload PDF (691K) -
Teruhiko HAGIWARA, Tetsuko YASUNO, Keiichi FUNAYAMA, Sukeji SUZUKI1997 Volume 38 Issue 4 Pages 211-218_1
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSLycopene, α-carotene and β-carotene in canned vegetable juice were determined by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry (LC/APCI-MS) with selected ion monitoring (SIM). The extracted carotenoids were separated on a C-18 reversed phase column with methanol as the mobile phase and cholesterol benzoate as an internal standard (IS). In order to obtain good repeatability, only the peak portion of analytes eluted from the LC column was introduced into the APCI interface by using an auto switching valve. In the positive mode, lycopene, α-carotene and β-carotene were monitored at m/z 537 and IS was monitored at m/z 369. The limit of detection was 1ng (s/n=4) and the recoveries of lycopene, α-carotene and β-carotene were 85.1%, 80.0% and 82.8%, respectively.
This SIM determination using LC/APCI-MS allows the analyte in samples to be determined without the influence of co-existing substances which cause problems in LC-UV chromatography.View full abstractDownload PDF (782K) -
Hisaya TERADA, Masaki MIYABE1997 Volume 38 Issue 4 Pages 219-224_1
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA fast semi-micro liquid chromatographic method for the simultaneous determination of hesperidin, naringin and their aglycones, i. e, hesperetin and naringenin, in processed foods was developed. The sample was refiuxed with 60% aqueous methanol on a hot water bath at 90°C and cleaned up on a Sep-Pak C18 cartridge. The sample solution was chromatographed on a semi-micro column (2.1mm i. d×80mm) packed with 3μm particles of Capcell Pak C18-SG120 with a mobile phase of acetonitrile-water-0.2mol/L phosphate buffer, pH 4.0 (18:77:5). Detection was achieved with a UV monitor set at 283nm.
The recoveries from soft drinks, marmalade, candy, yogurt, cookies and salad dressing fortified with the standard mixture at levels of 4 to 100μg/g were 82.6 to 100.4% (C. V. 1.6-5.2%) for hesperidin, 88.3 to 98.9% (C. V. 0.7-2.4%) for naringin, 88.3 to 93.4% (C. V. 1.3-3.9%) for hesperetin and 91.2 to 96.7% (C. V. 1.1-2.6%) for naringenin. The detection limit was 0.5μg/g for each compound.View full abstractDownload PDF (705K) -
Jeongeun CHOI, Myung Ja CHOI, Choonmi KIM, Jongkyu HA, Byungju HONG, Y ...1997 Volume 38 Issue 4 Pages 225-232_1
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThis study was performed to investigate the effects of recombinant bovine somatotropin (rBST) administration on residual BST and insulin-like growth factor-I (IGF-I) levels in various tissues. Two experiments, (A) and (B) were conducted as follows. (A) Beef cattle (average b. w. of 450kg) were divided into three groups: control (CONT), low-dose (LOW) and high-dose (HIGH) groups. The CONT group received neither rBST nor vehicle. The LOW and HIGH groups were treated for 20 weeks by s. c. injection with 250mg rBST at one-week intervals and 500mg rBST at two-week intervals, respectively. (B) Beef cattle were divided into four groups: control (CONT) group, as described in experiment (A), and sustained-release, low-dose (SR-L), sustained-release, medium-dose (SR-M) and sustained-release, high-dose (SR-H) groups. SR-L, SR-M and SR-H were treated at two-week intervals for 24 weeks by s. c. injection with doses of 0.42mg rBST/kg b. w. (0.03mg rBST/kg b. w./day), 0.84mg rBST/kg b. w. (0.06mg rBST/kg b. w./day) and 1.26mg rBST/kg b. w. (0.09mg rBST/kg b. w./day), respectively. In both experiments, animals were killed two weeks after the final treatment.
In experiment (A), residual BST and IGF-I levels in muscle tissues of the LOW and HIGH groups were not affected by rBST administration. In experiment (B), residual BST and IGF-I levels in muscle, fat, liver and kidney tissues of treated groups were not different from that of the CONT group, either. These results suggest that levels of BST and IGF-I in various tissues are not increased at two weeks after rBST administration.View full abstractDownload PDF (900K) -
Hitoshi MIYAZAKI, Masao ABE, Masaharu ASANOMA, Yuji NAGAI, Masahiro NA ...1997 Volume 38 Issue 4 Pages 233-239_1
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple method was developed for the analysis of carbon monoxide (CO) in fish meat. The frozen (or raw) sample was minced and weighed into a screw cap vial. After addition of 1-octanol and 10% sulfuric acid, the vial was incubated at 40°C in a water bath for 5min, then shaken vigorously for 15min to release the CO gas. CO was analyzed by head-space gas chromatography. The detection limit of CO was 2μg/kg for fish meat.
The developed method was applied to the determination of CO in tilapia, tuna and other kinds of fish meat. This simple method is convenient for the rapid determination of CO content in fish meat.View full abstractDownload PDF (805K) -
Yukihiro GODA, Hiromichi NAKAMURA, Shiho SASAKI SAKAMOTO, Keiko ISHIKA ...1997 Volume 38 Issue 4 Pages 240-247_1
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSCapsanthin (CP) and esterified capsanthins are the main coloring constituents in paprika color. Several reports have suggested that esterified capsanthins are more stable to light than CP itself in paprika powder and paprika extract. To clarify whether this is so, the photo-stability of lauroylmyristoylcapsanthin (LM-CP) and CP was investigated in paprika powder, commercial paprika solution and isolated individual solutions. After irradiation of the samples with visible light (4, 000lux) at 25°C, HPLC analyses were performed to quantify both compounds.
The degradation curves of these compounds in paprika powder and commercial paprika solution were different. Namely, the amount of LM-CP began to decrease after photo-irradiation for several weeks, while that of CP decreased with time. On the other hand, the curves for both isolated compounds were quite similar in the same solvent.
To resolve the discrepant observations, we precisely checked the chromatograms of paprika powder and commercial paprika solution after photo-irradiation. A peak, which appears at 68.3min, about 2min after the peak of LM-CP in the chromatograms, decreased quickly during an early stage of photo-irradiation and the peak of LM-CP significantly increased concomitantly. HPLC analyses revealed that the isolated fraction corresponding to the peak at 68.3min was transformed to LM-CP by photo-irradiation. The LC-APCI-MS analysis of the peak (68.3min) showed a quiasi molecularion peak (m/z 978), which was also observed in LM-CP. These data suggested that the peak at 68.3min is due to a geometrical isomer of LM-CP and that the photo-isomerization of the isomer to LM-CP occurred along with the photo-degradation of LM-CP.
We conclude that LM-CP and CP do not differ significantly from each other in photo-stability. When samples contained a significant amount of geometrical isomer of LM-CP, an additional amount of LM-CP was generated by photo-irradiation. Consequently, degradation of LM-CP was apparently delayed, compared with that of CP. It was considered that these phenomena led erroneous interpretation of the stability of esterified capsanthins.View full abstractDownload PDF (958K) -
Yoko UEMATSU, Masako HIROKADO, Keiko HIRATA, Yuki SADAMASU, Kazuo YASU ...1997 Volume 38 Issue 4 Pages 248-258_1
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple method was developed for the determination of monoglycerides (MG) and propylene glycol esters of fatty acid (PGE) in food additive preparations without derivatization, using a gas chromatograph equipped with a deactivated inlet tube, by splitless injection. A wide-bore capillary column of 0.53mm i. d. was used for separation, and a flame ionization detector was used for detection. GC/MS (CI) and GC/FTIR were used for identification. Tetrahydrofuran was used as the extracting solvent, and hexane, diethyl ether and ethyl acetate as eluents. Monocaprylin, monocaprin, monopalmitin, monostearin, monoolein and monolinolein were detected in commercial food additive preparations.View full abstractDownload PDF (1174K) -
Shuzo TAGUCHI, Seisaku YOSHIDA, Shigehiko FUKUSHIMA, Shinjiro HORI1997 Volume 38 Issue 4 Pages 259-264_1
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA rapid and simple method for the analysis of four kinds of tetracycline antibiotics (TCs), oxytetracycline, tetracycline, chlortetracycline and doxycycline, was established by HPLC using an on-line clean-up system. Co-existing substances in milk were removed by a precolumn head-cut-loading method. TCs retained in the precolumn were rapidly loaded on an analytical column after changing the eluent. The co-existing substances retained in the precolumn was removed by back-flash washing. Sodium octanesulfonate was effective as a counter ion in the mobile phase for separation of TCs on the analytical column. Determination of TCs in milk by this method could be carried out only after deproteinization with 20% trifluoroacetic acid. Recoveries of TCs from milk fortified at 0.03, 0.05, 0.1, 0.5 and 1.0mg/L ranged from 80.2 to 104.3%. Detection limits were 0.01mg/L for all four TCs.View full abstractDownload PDF (693K) -
Masatake TOYODA, Makoto MIYAHARA, Hiroshi AKIYAMA, Yukio SAITO1997 Volume 38 Issue 4 Pages 265-269_1
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSNew detection methods for particulate matter in bottled mineral waters were studied.
A turbidity index method using a simple and sensitive turbidimeter involves taking a 100mL sample of bottled mineral water and comparing its turbidity with those of reference standard solutions. It is possible to discriminate mineral waters showing a turbidity index of as small as 0.01 and contaminants floating in the mineral waters can be easily detected.
A particle counter method involves passing 300mL of bottled mineral water through a light-scattering type sensor at a flow rate of 50mL/min. The size distributions of particulate matter in bottled mineral waters measured by this method were similar to those measured by a membrane filter method.
Both methods appear to be suitable for monitoring the quality of bottled mineral waters.View full abstractDownload PDF (604K) -
Toshihiro NAGAYAMA1997 Volume 38 Issue 4 Pages 270-274_1
Published: August 05, 1997
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSFruit wines were produced using fruits which contained residual organophosphorus pesticides. Some of the dialifos and prothiofos contained in strawberries and some ethion in grapefruit was transferred into the fruit wines (1 to 7%, respectively). The content of pesticides in the wines was maximum after soaking for one day, and then gradually decreased.
The rate of this decrease was well described by the following first-order equation.
log (Tr)=A-0.4×log(D)
where Tr is the content ratio of pesticide transferred from the fruit to the wine (%), A is the content transferred to the wine after one day of soaking, and D is the number of days of soaking.View full abstractDownload PDF (624K)
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