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Volume 44 (2003)
- Issue 6 Pages 269-
- Issue 5 Pages 213-
- Issue 4 Pages 181-
- Issue 3 Pages 145-
- Issue 2 Pages 77-
- Issue 1 Pages 1-
Volume 44, Issue 4
Displaying 1-6 of 6 articles from this issue
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Originals
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Yasuo TANAKA, Kyoko TAKAHASHI, Tadayoshi SASAO, Tadashi KIRIGAYA, Shir ...2003 Volume 44 Issue 4 Pages 181-185
Published: August 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSTwo peaks, A and B, detected in chromatograms of commercial frozen vegetable extracts during analysis of organophosphorus pesticide residues by GC-FPD, were identified as tris(2,4-di-tert-butylphenyl)phosphite (Irgafos 168) and Irgafos 168 oxide, respectively, from their mass spectra. Irgafos 168 is used as an antioxidant in plastics, and there has been no report of its detection in foods. We analyzed Irgafos 168 and its oxide in 38 samples of commercial frozen vegetables, and they were detected from 4 samples (0.02∼0.80 μg/g as total amount of Irgafos 168 and its oxide).View full abstractDownload PDF (501K) -
Koji YAMASHITA, Yuko KANAZAWA, Michi UENO, Hiromoto OHTA, Michiyo KITA ...2003 Volume 44 Issue 4 Pages 186-190
Published: August 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSIn June 2000, there was a large-scale outbreak of food poisoning after consumption of Snow Brand low fat milk. In the evening of a day the incident made public, some cartons of low fat milk were brought to our laboratory for examination. Next day, we detected only staphylococcal enterotoxin (SE) A gene among SE (A∼E) genes by PCR in left-over milk samples or samples from the same lots that patients had consumed. We presumed that the outbreak was caused by the intake of SEA. We subsequently confirmed the presence of SEA in these samples.
To investigate the existence of SE (A∼E) genes in milk, we examined 100 samples of commercial low fat milk and milk by PCR, but none of the genes was detected. We estimated the detection limit of SEA gene in low fat milk by PCR. Four strains of SEA-producing Staphylococcus aureus cultures were serially diluted in low fat milk. The SEA gene was detected at levels of 5.5×102 to 1.6×104 cfu/mL of S. aureus. These amounts of S. aureus are higher than the values in raw milk reported previously. Therefore we consider that SE genes in low fat milk should usually be undetectable by our PCR.
This study shows that quick detection of SE genes by PCR is very helpful to analyze outbreaks, especially if no significant bacterium can be cultured.View full abstractDownload PDF (1082K) -
Maki MIYAZAWA, Chieko MIYAHARA, Shuji SATOH, Anri SAKAI2003 Volume 44 Issue 4 Pages 191-197
Published: August 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSMulberry leaf extract was studied toxicologically in male and female SD rats. The extract was administered orally at concentrations of 0% (control group), 0.1%, 0.4% and 1% in basal diet for 90 days. No remarkable change in test animals of both sexes was observed in terms of body weight gain or at necropsy. Hematology and blood chemistry revealed no abnormalities. Pathological examination revealed no toxic change in any organ observed. These findings indicate that dietary intake of 1% mulberry leaf extract for 90 days (884.5 mg/kg/day for males and 995.7 mg/kg/day for females as mean daily intake) causes no toxicological change in rats.View full abstractDownload PDF (602K)
Notes
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Naoyuki SATO, Keiko ISHII, Akio SATOH, Toshio HIDAKA, Noboru NAGAOKA2003 Volume 44 Issue 4 Pages 198-202
Published: August 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSWe have analyzed moxidectin residues in beef by a rapid and highly sensitive liquid chromatography-mass spectrometry (LC/MS) method without fluorescent derivatization. By using atmospheric pressure chemical ionization (APCI) with a fragment voltage of 100 V, good measurement results were obtained and the recoveries of moxidectin were 93.3% and 96.5% from adipose and muscle tissues, respectively. Identification of moxidectin residues in commercial beef was performed using three monitor ions. When quantitated at m/z 622, the levels of moxidectin were 35 ng/g in adipose tissue and 4.3 ng/g in muscle tissue. After adjustment for fat content and calculation of the moxidectin concentration on a fat basis, it was found that the moxidectin contents were the same in adipose and muscle tissues. Thus, it is considered that residual levels of moxidectin in adipose and muscle tissues are correlated to the fat contents in the tissues.View full abstractDownload PDF (538K) -
Hiroko SHIODA, Kanako SATOH, Fumiko NAGAI, Tomoko OKUBO, Takako SETO, ...2003 Volume 44 Issue 4 Pages 203-207
Published: August 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSJuice and integument of leaves of 3 Aloe species, Aloe vera, A. ferox and A. africana, are not allowed to be used as food according to the Pharmaceutical Affairs Law in Japan. On the other hand, whole leaves of A. arborescens can be used as food. The present study was designed to distinguish Aloe species by random amplified polymorphic DNA (RAPD) analysis. DNA was isolated from fresh and dried leaves of the 4 Aloe species. Five out of 32 different 10-mer primers examined were useful for analysis. By comparison of the characteristic bands of PCR products on agarose gel, it was possible to distinguish the 4 species. Thus, the botanical species of Aloe in commercial food products can be identified by RAPD analysis.View full abstractDownload PDF (1008K)
Report
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Hiroyuki OHNO, Masako SUZUKI, Taiki AOYAMA, Kazunori MITANI2003 Volume 44 Issue 4 Pages 208-212
Published: August 25, 2003
Released on J-STAGE: January 21, 2009
JOURNAL FREE ACCESSOrganotin compounds in polyvinyl chloride toys were determined by GC/MS after ethyl derivatization with sodium tetraethylborate. The samples were 12 balls, 12 soft toys, 10 food toys and 13 face masks for children.
Monooctyltin, dioctyltin and trioctyltin compounds were found in all face masks at the levels of 74.8∼917 μg/g, 474∼3,960 μg/g and 1.0∼213 μg/g, respectively. They also were detected in 6 balls, 4 soft toys and 1 food toy. Monomethyltin and dimethyltin compounds were found in 8 face masks at the levels of 40.9∼227 μg/g and 222∼1,450 μg/g, respectively. Monobutyltin and dibutyltin (DBT) compounds were found in 1 ball, 3 food toys and 5 face masks. In particular, 1 ball and 4 face masks contained toxic DBT at the levels of 527∼999 μg/g.View full abstractDownload PDF (497K)
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