All issues
Volume 50 (2009)
- Issue 6 Pages 279-
- Issue 5 Pages 191-
- Issue 4 Pages 153-
- Issue 3 Pages 109-
- Issue 2 Pages 47-
- Issue 1 Pages 1-
Volume 50, Issue 3
Displaying 1-7 of 7 articles from this issue
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Originals
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Hirokazu OGIHARA, Asako TUYUKI, Soichi FURUKAWA, Yasushi MORINAGA, Shi ...2009 Volume 50 Issue 3 Pages 109-116
Published: June 25, 2009
Released on J-STAGE: July 15, 2009
JOURNAL FREE ACCESSThe effect of the reconstruction and storage conditions of powdered infant formula (PIF) on the survival and growth of three Enterobacter sakazakii strains, ATCC 29004, HT 022 and HT 028, was investigated. D values of E. sakazakii ATCC 29004 and HT 022 at 60°C were 3.6 and 1.6 min, respectively, and that of HT 028 at 52°C was 1.6 min. The effect of the temperature of the water used for the reconstruction of PIF on the inactivation of the three E. sakazakii strains was also investigated. One to 2 log order inactivation occurred at 70°C, and above 5 log order inactivation at 80°C. Storage tests at 5, 10 and 25°C showed that none of the strains could grow at 5°C, HT 028 grew slightly at 10°C, and at 25°C all three strains started growth after 4 hr incubation and reached up to 8 log CFU/mL after 16 hr incubation. From the above results, it is concluded that a suitable temperature of the hot water for reconstruction of PIF is above 70°C, and the preferred storage temperature of reconstructed PIF, which is recommended to be consumed within 2 hr, is below 5°C.View full abstractDownload PDF (773K) -
Taichi OGUCHI, Mari ONISHI, Yasutaka MINEGISHI, Yasunori KUROSAWA, Mas ...2009 Volume 50 Issue 3 Pages 117-125
Published: June 25, 2009
Released on J-STAGE: July 15, 2009
JOURNAL FREE ACCESSA duplex real-time PCR method was developed for quantitative screening analysis of GM maize. The duplex real-time PCR simultaneously detected two GM-specific segments, namely the cauliflower mosaic virus (CaMV) 35S promoter (P35S) segment and an event-specific segment for GA21 maize which does not contain P35S. Calibration was performed with a plasmid calibrant specially designed for the duplex PCR. The result of an in-house evaluation suggested that the analytical precision of the developed method was almost equivalent to those of simplex real-time PCR methods, which have been adopted as ISO standard methods for the analysis of GMOs in foodstuffs and have also been employed for the analysis of GMOs in Japan. In addition, this method will reduce both the cost and time requirement of routine GMO analysis by half. The high analytical performance demonstrated in the current study would be useful for the quantitative screening analysis of GM maize. We believe the developed method will be useful for practical screening analysis of GM maize, although interlaboratory collaborative studies should be conducted to confirm this.View full abstractDownload PDF (668K) -
Junko UI, Kazuo KONDO, Takuo SAWADA, Yukiko HARA-KUDO2009 Volume 50 Issue 3 Pages 126-130
Published: June 25, 2009
Released on J-STAGE: July 15, 2009
JOURNAL FREE ACCESSWe investigated the survival of bacterial pathogens in grain products. Salmonella Enteritidis and Staphylococcus aureus survived for more than 3 weeks in grain flakes. Although S. Enteritidis in grain flakes reached a level of less than 102 CFU/g, the surviving organisms grew rapidly in milk and reached 109 CFU/g after 25-hr incubation. When catechins were present on the grain flakes, the numbers of S. Enteritidis and S. aureus were significantly decreased. In addition, catechins reduced the survival and growth of S. Enteritidis, S. aureus and Bacillus cereus when added to cooked grain products. This study indicates that S. Enteritidis, S. aureus and Bacillus can survive for a long time in grain products and that catechins impair their survival and growth.View full abstractDownload PDF (238K)
Notes
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Mizuka FUJITA, Kensaku KAKIMOTO, Haruna NAGAYOSHI, Yoshimasa KONISHI, ...2009 Volume 50 Issue 3 Pages 131-134
Published: June 25, 2009
Released on J-STAGE: July 15, 2009
JOURNAL FREE ACCESSA method using liquid chromatograph coupled with tandem mass spectrometer (LC/MS/MS) was developed for the determination of melamine in processed food. After extraction with 50% acetonitrile and clean-up with PSA and SCX, the quantification limit of melamine in processed food was 0.5 μg/g. The recoveries and relative standard deviations were 113 to 115% and less than 5%, respectively. Therefore, we considered that the developed method offers high precision and sensitivity for the determination of melamine in processed food. When the method was applied to six suspected products, 0.8 to 37 μg/g of melamine was detected from four milk-rich productsView full abstractDownload PDF (374K) -
Yoshiharu IHARA, Mihoko KATO, Tsukasa KODAIRA, Shinji ITOH, Mika TERAK ...2009 Volume 50 Issue 3 Pages 135-139
Published: June 25, 2009
Released on J-STAGE: July 15, 2009
JOURNAL FREE ACCESSA sample preparation method was developed for determination of quinolones in honey using immunoaffinity resin. For this purpose, an immunoaffinity resin for quinolones was prepared by coupling a quinolone-specific monoclonal antibody to agarose resin. Honey samples diluted with phosphate buffer were reacted with immunoaffinity resin. After the resin was washed, quinolones were eluted with glycine-HCl. Quinolones in the eluate were determined by HPLC with fluorescence detection. No interfering peak was found on the chromatograms of honey samples. The recoveries of quinolones from samples were over 70% at fortification levels of 20 ng/g (for norfloxacin, ciprofloxacin and enrofloxacin) and 10 ng/g (for danofloxacin). The quantification limits of quinolones were 2 ng/g. This sample preprocessing method using immunoaffinity resin was found to be effective and suitable for determining residual quinolones in honey.View full abstractDownload PDF (517K)
Reports
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Yoko OHGIYA, Masaaki SAKAI, Taeko MIYASHITA, Koichi YANO2009 Volume 50 Issue 3 Pages 140-145
Published: June 25, 2009
Released on J-STAGE: July 15, 2009
JOURNAL FREE ACCESSGenetically modified maize MON863 (MON863), which has passed a safety examination in Japan, is commercially cultivated in the United States as a food and a resource for fuel. Maize is an anemophilous flower, which easily hybridizes. However, an official method for quantifying the content of MON863 has not been provided yet in Japan. We here examined MON863 contamination in maize-processed foods that had no labeling indicating of the use of genetically modified maize.
From March 2006 to July 2008, we purchased 20 frozen maize products, 8 maize powder products, 7 canned maize products and 4 other maize processed foods. Three primer pairs named MON 863 primer, MON863-1, and M3/M4 for MON863-specific integrated cassette were used for qualitative polymerase chain reaction (PCR). A primer pair “SSIIb-3” for starch synthase gene was used to confirm the quality of extracted DNA. The starch synthase gene was detected in all samples. In qualitative tests, the MON863-specific fragments were detected in 7 (18%) maize powder products out of the 39 processed foods with all the three primer pairs.
We concluded that various maize processed foods on the market were contaminated with MON863. It is important to accumulate further information on MON863 contamination in maize-processed foods that have no label indication of the use of genetically modified maize.View full abstractDownload PDF (681K) -
Kyoko KAMIJO, Maki KOBAYASHI, Kenji OTSUKA, Yasuhiro TAMURA, Sanae TOM ...2009 Volume 50 Issue 3 Pages 146-152
Published: June 25, 2009
Released on J-STAGE: July 15, 2009
JOURNAL FREE ACCESSPesticide residues in 165 imported vegetable products on the Tokyo market from Apr. 1992 to Mar. 2006 were investigated. Seven kinds of pesticides were detected at levels between Tr (below 0.01 ppm) and 1.0 ppm from 16 samples. There was no sample in which pesticide residues exceeded the MRLs. The salt leaf of grape imported from Greece contained chlorpyrifos and quinalphos, and there was some doubt as to whether the residue levels exceeded the MRLs when the Japanese positive list system for pesticide residues in food was applied. Pesticides were detected from pickles and dehydrated vegetable, but not from products that had been treated with heat and water, such as boiled vegetables and purees. Many samples of products from Asia, and North America area contained detectable levels of pesticides. Residue levels of these pesticides were calculated as between 0.006 and 20.3% of their ADI values according to the daily intake of vegetable products. Therefore, these vegetable products should be safe when eaten in a normal manner.View full abstractDownload PDF (682K)
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