巻号一覧
後続誌
48 巻 (2004)
- 4 号 p. 123-
- 3 号 p. 81-
- 2 号 p. 55-
- 1 号 p. 1-
- Supplement 号 p. 2-
48 巻, 3 号
選択された号の論文の7件中1～7を表示しています
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河合 忠2004 年 48 巻 3 号 p. 81-84
発行日: 2004/09/15
公開日: 2009/03/31
ジャーナル フリーProtein science has been advanced with new development of various separation and analytical techniques, and one of the most useful techniques at the beginning was various electrophoretic fractionation. In 1973, Watson & Crick reported on a structure of deoxyribonucleic acid, and then molecular biology has progressed rapidly. In 1991, the international cooperative project on human genome started, and ended in 2003, reporting more than 99% of the whole base sequence of human genome. However, the project has left most of human genes functionally unknown. Currently the major research in life science is aiming to determine biological functions of individual proteins as well as regulatory networks among many proteins, namely “proteomics”.抄録全体を表示PDF形式でダウンロード (737K) -
田渕 眞理, 蔵満 保宏, 中村 和行, 馬場 嘉信2004 年 48 巻 3 号 p. 85-87
発行日: 2004/09/15
公開日: 2009/03/31
ジャーナル フリーHigh-throughput with high quality proteome analysis is necessary for proteomics research and medical diagnosis. In this paper, we introduce the view of advanced microchip electrophoresis for clinical application in proteomics research. Protein size separation based on sodium dodecyl sulfate-gel electrophoresis (SDS-GE) requires denaturing, but we propose that denaturing is unnecessary for analysis by microchip electrophoresis. We attained dramatically improved sensitivity without compromising size determination by omitting the protein denaturing process. Protein expression of Jurkat cells during stress-shock induced apoptosis was readily analyzed using this system. We found that a food extraction effectively induced apoptosis of Jurkat cells. This system will accelerate the proteomics research and will be utilize for the medical diagnosis in future.抄録全体を表示PDF形式でダウンロード (497K) -
必要とされる技術開発と臨床研究への応用近藤 格2004 年 48 巻 3 号 p. 89-91
発行日: 2004/09/15
公開日: 2009/03/31
ジャーナル フリーTwo-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has a great utility to develop clinical markers, as it uncovers many types of multiple proteins in quantitative, reproducible and high throughput manner. Current technological progress around 2D-PAGE, such as fluorescence dyes, bioinformatics, laser microdissection, enable more sophisticated and accurate proteomic study. For instance, the tumor marker for unknown-origin tumor will be developed by identifying the expression pattern of spots. Although numerous challenges still lie ahead, the future of proteomics, especially medicine-oriented proteomics, by 2D-PAGE looks promising.抄録全体を表示PDF形式でダウンロード (479K) -
藏満 保宏, 中村 和行2004 年 48 巻 3 号 p. 93-97
発行日: 2004/09/15
公開日: 2009/03/31
ジャーナル フリーChronic infection with hepatitis C virus (HCV) is one of the most clearly established risk factors for hepatocellular carcinoma (HCC). The number of HCV-carriers in Japan is estimated to be 2 million and 32, 000 of HCC patients pass away every year. Therefore, it is important to clarify the mechanism involved in hepatocarcinogenesis by chronic HCV infection.
We performed proteomic studies using samples of tumor tissues and non-tumor tissues from HCC patients with HCV infection but not hepatitis B virus infection. This study using two-dimensional gel electrophoresis with MALDI-TOF-MS showed that HSP70 family such as GRP78, HSC70, GRP75 and HSP70.1, glutamime synthetase isoforms, alpha-enolase, phosphoglycerate mutase, and triosephosphate isomerase were increased but albumin, ferritin light chain, smoothelin, tropomyosin beta chain, arginase 1, aldolase B and ketohexokinase were decreased in the tumor tissues.抄録全体を表示PDF形式でダウンロード (4049K) -
血管新生制御因子, 癌特異自己抗体など疾患関連蛋白の同定中西 豊文2004 年 48 巻 3 号 p. 99-103
発行日: 2004/09/15
公開日: 2009/03/31
ジャーナル フリー2次元電気泳動-ウエスタンブロット-ソフトイオン化質量分析法により, 血管新生制御因子を見出した. また, 抗 Aldehyde Dehydrogenase 抗体など今回見出した肺癌細胞特異蛋白質群の経時的変化を明らかにすることにより, 治療効果判定や早期診断に有用な情報をもたらすものと思われた.抄録全体を表示PDF形式でダウンロード (3748K) -
坂口 和子, 鈴木 潤, 田中 雅樹, 赤堀 文昭2004 年 48 巻 3 号 p. 105-109
発行日: 2004/09/15
公開日: 2009/03/31
ジャーナル フリーChanges in rat plasma proteins after administration of paraquat (PQ) were observed by micro two-dimensional polyacrylamide gel electrophoresis (Type II), under denaturing conditions. In the area of molecular weight lower than that of albumin, there were increases in four spots of plasma proteins, i.e., those at isoelectric points (pI) 5.0 and 5.9 with a molecular weight (MW) of ca. 54, 000, and 5.7 and 7.5 with a MW of ca. 15, 000, in the rats administered PQ. On the other hand, there were decreases in two spots of plasma proteins, i.e., those at pI 5.0-6.0 with a MW of ca. 54, 000 and 5.0 with a MW of ca. 18, 000. The protein spot at the pI 5.0 with a MW of ca. 18, 000 was identified as α2u-globulin (AUG) by MALDI-TOF/MS/MS.
Some rats administered PQ showed the marked decrease in AUG, and pathological findings of pulmonary fibrosis were also observed. These results suggest the involvement of AUG with PQ-induced pulmonary fibrosis.抄録全体を表示PDF形式でダウンロード (7086K) -
梶原 英之, 杉村 嘉男2004 年 48 巻 3 号 p. 111-115
発行日: 2004/09/15
公開日: 2009/03/31
ジャーナル フリーA new technique for tube-gel isoelectric focusing (IEF) using plastic tubes instead of glass tubes in the first dimension of two-dimensional gel electrophoresis (2-DE) was developed for proteomic analysis of both denatured and non-denatured proteins. The plastic tubes have advantages in handling gels over glass tubes. The plastic tubes were also applied successfully to native gel electrophoresis, blue-native gel electrophoresis, agarose gel IEF, acid-urea gel electrophoresis and cetyltrimethylammonium bromide (CTAB) gel electrophoresis for the first dimension of 2-DE.抄録全体を表示PDF形式でダウンロード (9077K)
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