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Volume 2, Issue 1
Displaying 1-7 of 7 articles from this issue
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Effect of cortisone on the susceptibility of mice to virus (2)YOH NAKAGAWA, YUKISHIGE KANDA1955 Volume 2 Issue 1 Pages 1-18
Published: August 01, 1955
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSIn the previous report (1) we confirmed that relatively large dose of cortisone, when employed as a preliminary treatment, had promoted the susceptibility of mice to mouse encephalomyelitis virus. As cortisone shows a very wide action, an unitary understanding on the mechanism of its action has not yet been reached in spite of many studies. Therefore, at the present time the mechanism of the susceptibility increasing effect of cortisone to virus is still unknown. As we pointed out in the previous report, cortisone promoted not only the susceptibility of mice to virus but also the susceptibility to all common bacteria, so a possibility that a single medicine works on various different pathogenic microorganisms is considered to be very little. Southan et al (2) concluded that infection promoting eflect of cortisone must have been the action on the host and we think that this point is quite right.We understand quite well that adrenal cortex plays an important role on maintaining life, but we are very doubtful that cortisone shows very wide action. So we would like to conceive that cortisone may cause a chain reaction in living bodies. In order to solve this problem there is no other means but to study step by step. We believe that the solution of this problem will serve a great deal for analysis of virus susceptibility.In this report, we wish to report the result on the effect of cortisone upon the multiplication of virus in the brain and the condition of mice at the time of administeration of cortisone.View full abstractDownload PDF (3708K) -
EIJI KIMOTO1955 Volume 2 Issue 1 Pages 19-22
Published: August 01, 1955
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSIn the preceding two works (1, 2) the present author studied on the interaction between various kinds of monolayers and alkaloids by the surface pressure and surface potential measurements and made some discussions on the mode of interaction between them. On the interaction between monolayer and dye, Matsuura (3) and Muramatsu (4) studied by means of the measurement of the pressure-area relation of stearic acid and octadecylamine monolayer on the dye solution.Both of the alkaloids and the dyes have generally a large molecular weight and basic nitrogen atoms (in basic and amphoteric dyes) but they are extremely different with each other in their molecular structures.So, it was intended in this work to investigate the interaction between stearic acid monolayer and dissolved dye by the surface pressure and surface potential measurements, to clarify further the mode of interaction between them and then to compare this result with those obtained in the case of alkaloids from the point of view of their molecular structures.View full abstractDownload PDF (3292K) -
YOH NAKAGAWA, AKIO AKASHI1955 Volume 2 Issue 1 Pages 23-31
Published: August 01, 1955
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSIt has been well known that the use of ultracentrifuge as the step of the purification of influenza virus is the most effective method, however, as the use of the apparatus is so far fairly limited in our country, other methods for purifying viruses as pure as possible from the infected materials without losing their activities should be investigated. We reported in the previous paper (1) that the purification of influenza virus was achieved to some extent by means of absorption and elution using such absorbents as ion exchange resin, clay, kaolin, diatom earth, or chicken red cells, and furthermore, that the virus was purified to a considerably high degree by combining precipitation methods, preliminary purified by these adsorbents.Purification of various toxins as well as viruses by means of precipitation and elution employing ZnCl2, KAl(SO4)2, protamin sulfate, or isoelectric point precipitation, has been reported by many workers. Miyata (2) purified tetanus toxin by ZnCl2 and Hosoya (3) and Borily (4) reported that precipitation and elution by KAl (SO4) 2 was useful for the purification of diphtheria toxin (3) and influenza virus (4) respectively. Chambers and Henle (5), Warren et al(6), Warren (7), and Nakagawa (8) employed protamine sulfate to purify partially various viruses with considerably good results. Other wokers proved that vaccinia virus (9) and poliomyelitis virus (10) in infected tissues could be fairly purified by the isoelectric point precipitation method.The present paper shows the results of purification of influenza virus by means of combination of those methods, described above, and methanol precipitation method which was conducted similarly or with slight change to that recently reported by Cox (11) and Fukumi (12).View full abstractDownload PDF (862K) -
YOH NAKAGAWA, MASAHIRO NAKAMURA1955 Volume 2 Issue 1 Pages 32-41
Published: August 01, 1955
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSIn recent years Lahelle and Horsfall (1) demonstrated that the GDVII strain of mouse encephalomyelitis virus (2) agglutinated human erythrocytes at 4 C, and indicated that a correlation between infectivity and hemagglutinative capacity associated in the virus particle itself. Lahelle and Ward (3) reported subsequently on purification and concentration of GDVII virus by using of hemagglutination. In the paper they tried concentration of the virus by hemagglutination, and reported that the adsorption and elution procedures seemed to give equally good or better results than the alcohol precipitation method. In the concentration of virus by hemagglutination, however, many problems to be studied, for example hemolysis, by elution which has not been so far reported have taken place.The purpose of the present paper is to investigate further more effective methods toward the purification of GDVII virus by means of ether extraction (4) (7), hemagglutination, and methanol (4) (5) (6) and acetone (7) precipitation at low temperature.View full abstractDownload PDF (942K) -
KAZUO SUENAGA, KEN NODA1955 Volume 2 Issue 1 Pages 42-50
Published: August 01, 1955
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSThe E.E.G. of human brain was firstly mentioned by HANS BERGER, German psychiartist, in 1929 as an epoch-making affair and at present time the students of E.E.G. seem to have two researching directions, i.e. on the one hand it is the study on the mechanism of origination of E.E.G. and on the other it is on the electrophysiological explanations of brain. In latter attempt E. E. G. is used as the indicator of cerebral functions and the method applied for this purpose is the stimulation method of the brain and the excited state appeared as the changes of E.E.G. by stimulation is called as 'response'.In preceding we reported that we could not obtain the electrical inhibitory figure with stimulation in the simpler structure of nervous centre (1) and the present experiment is a report on a series of observation of inhibition or desynchronization of E.E.G.. Hence the authors investigated the relationship between temporal E.E.G. as generalized nervous function of a higher order and intermittent peripheral sound stimuli.View full abstractDownload PDF (5678K) -
II. Effect of bovine albumin fraction V on the growth of acid fast bacillus and some experiments for determination of the mechanism of its promoting effectMASAHIRO NAKAMURA1955 Volume 2 Issue 1 Pages 51-62
Published: August 01, 1955
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSIn the previous reports (1) (2) (3), the effect of bovine serum and egg albumin on the growth of Mycobacterium phlei has been investigated by electrophoresis using a Tiselius apparatus. The results obtained demonstrated that these proteins which promote the growth of the organisms acted as catalytic or protective rather than nutritive growth factors, because the quantitative and qualitative changes of albumin in the media after ten days of inoculation of Mycobacterium phlei was not observed by means of electrophoretic patterns. This paper reports the effect of bovine albumin fraction V on the growth of Mycobacterium phlei and BCG, observation on the mechanism of the stimulating effect of the albumin, and furthermore, describes whether this electrophoretic method would be reasonable or not for the determination of mechanism of the promoting effect of albumin on the growth of acid fast bacilli.View full abstractDownload PDF (10642K) -
YOSHIO YOSHIZUMI, ICHIRO KURODA, HIROMI WATANABE, TAKUGORO ODA1955 Volume 2 Issue 1 Pages 63-75
Published: August 01, 1955
Released on J-STAGE: August 11, 2009
JOURNAL FREE ACCESSThe decisive diagnosis of schistosomiasis japonica should be made by detecting eggs in the patient. One of the authors YOSHIZUMI pointed out already that the fresh liver slide method, which he provisionally named (a very small piece, which is removed from the liver tissue by means of biopsy is placed on a slide and is covered with a coverglas and is examined under a microscope) had an advantage over the old in detecting eggs quickly and decisively (1).From July 1954 until March 1955, by the above mentioned method we examined 113 patients, who called on our hospital from the schistosomum japonocum infested area (Kurume-shi and its vicinity), south and north Shigeyasu-mura, Miyaki-gun and Kisato-machi, Tosu-shi (Saga-ken).View full abstractDownload PDF (9903K)
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