生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
27 巻, 3 号
選択された号の論文の7件中1~7を表示しています
  • 橋本 信也, 揚塩 正樹, 堀田 正一, 能勢 俊一, 松本 尚美, 小林 敏子, 阿部 正和
    1983 年 27 巻 3 号 p. 109-118
    発行日: 1983/07/25
    公開日: 2009/03/31
    ジャーナル フリー
    Change of surface charge of the tanned sheep erythrocytes added with supernatants of human lymphocytes stimulated with antigens, mitogens or immunopotentiators was investigated. In supernatants of the sensitized lymphocytes stimulated with PPD, percentage slowing of electrophoretic mobility of sheep erythrocytes were increased. In normal lymphocytes cultured with PHA and Con A, there were high percentage slowings in electrophoretic mobility test, and in immunopotentiators such as OK-432, PSK, LVS, methyl B12, poly-A:U, poly-I:C and NaIO4, percentage slowings were not significantly changed.
    Percentage slowing was reduced in RA lymphocytes stimulated with PHA, but not changed in SLE lymphocytes. In normal lymphocytes incubated with Lentinan, percentage slowing value was similar to the result obtained by Con A, but it was low in lymphocytes from the patients with collagen diseases and malignancies.
    In SLE lymphocytes added with DNA from calf thymus and RA lymphocytes added with human IgG, electrophoretic mobility of sheep erythrocytes delayed.
  • 橋本 信也, 揚塩 正樹, 堀田 正一, 能勢 俊一, 松本 尚美, 小林 敏子, 阿部 正和
    1983 年 27 巻 3 号 p. 119-123
    発行日: 1983/07/25
    公開日: 2009/03/31
    ジャーナル フリー
    The supernatants of human peripheral blood lymphocytes stimulated with antigen and non specific mitogen influence electrophoretic mobility of sheep erythrocytes as indicator cells of lymphocyte sensitization. This test system has been used for application to clinical immunology.
    Lymphocytes from human peripheral blood were obtained by centrifugation in Ficoll-Conray method. Then separation of T and non T lymphocytes was performed by the ordinary E-rosetting technique. Separated T and non T lymphocyte fractions were incubated with PHA, Con A and DNA obtained from calf thymus. To cultured supernatants the tanned sheep erythrocytes were added, and electrophoretic mobility of the sheep erythrocytes was measured in an analytical cell microelectrophoretic apparatus.
    In the supernatant from normal T lymphocytes stimulated with PHA and Con A, decrease of electrophoretic mobility of the sheep erythrocytes was more remarkable than in that from normal non T lymphocytes. In the supernatants of the lymphocyte subpopulation from patients with SLE stimulated with Con A, delay of electrophoretic mobility of the sheep erythrocyte was observed in non T lymphocytes. When the lymphocytes from the patients with SLE were stimulated with DNA, electrophoretic mobility of the sheep erythrocyte showed change in the supernatants from T lymphocyte culture.
  • 藤原 弥栄子, 戸沢 辰雄
    1983 年 27 巻 3 号 p. 125-130
    発行日: 1983/07/25
    公開日: 2009/03/31
    ジャーナル フリー
    LDH結合IgGのProtein A-Sepharose CL-4Bに対する吸着能を,12例の患者血清で調べた.
    患者血清をProtein A-Sepharose CL-4Bとインキュベートし,その沈殿物中のLDH活性を測定した.その結果,全例に,Protein A-Sepharose CL-4B中のLDH活性が認められ,12例すべてのLDH結合IgGがProtein A-Sepharose CL-4Bへの吸着性をもつことが示された.
    Protein A-Sepharose CL-4Bを用いたLDH結合IgGの検出は簡便で,検出感度もすぐれている.しかも,IgGはProtein Aと一定の割合で結合し,LDH 1分子がIgG 1分子と結合していることから,LDH結合IgGを定量的に把握しうる.この方法を用い,LDH結合IgGの臨床経過に関連した動態を定量的に観察することは,この複合物の病態生理学的な解明に役立つものと期待できる.
  • 安川 薫, 小島 清嗣, 真鍋 敬, 奥山 典生
    1983 年 27 巻 3 号 p. 131-138
    発行日: 1983/07/25
    公開日: 2009/03/31
    ジャーナル フリー
    A new type apparatus was designed for carrier free isoelectric focusing which could be connected to high-performance liquid chromatography. The merits of this apparatus are as follows:
    1) The electroosmosis was low due to the plastic quality of electrophoresis tubing instead of the glass tubing.
    2) An amount of sample could be increased by increasing the number of tubings for electrophoresis.
    3) For fractionation, this apparatus was connected by pumping-out device with peristaltic pump to a UV detector and a fraction collector.
    For simplification and quick operation of this apparatus, we studied on basic conditions and on the separation of proteins by isoelectric focusing using carrier free electrophoresis. With incorporation of prefocusing process at high voltage, whole operation time could be shortened to one hour or less.
  • 谷口 孝治
    1983 年 27 巻 3 号 p. 139-149
    発行日: 1983/07/25
    公開日: 2009/03/31
    ジャーナル フリー
    An oral fat tolerance test was carried out in 16 healthy men. The lipid concentrations of plasma and each lipoproteins, and apoproteins in VLDL and HDL2 were analysed.
    The concentration of VLDL-TG was significantly increased 2 hours after lipid ingestion. A correlation between lipoprotein-lipids and apoproteins were observed, i. e., a positive correlation was found between HDL2-TG and apo-CII, and HDL2-chol. and apo E1. A reverse correlation was found between HDL2-TG and CIII0, and LDL-TG and CII & CIII0.
    The prevalence of apo E4 was found in 18.7% of the healthy men. However, there was a 22 year old man whose apo E4 was not observed before the tolerance test, whereas it was observed after the test (Case 1). These suggest that an appearance of apo E4 is not only due to an autosomal dominant transmission but also to other factors, such as diet, diseases of endocrine and metabolism.
    A deficiency of apo E3 was found in a 21 year old man (Case 2) who had a type IV hyperlipoproteinemia. It leads to a possibility that the deficiency of apo E3 is not a single disease, and is easy to complicate with variable hyperlipoproteinemia.
    An oral fat tolerance test has possibility to discover a latent hyperlipoproteinemia, and is a clinicaly useful test.
  • 今井 利夫, 吉田 光孝, 鈴木 光一, 降矢 震
    1983 年 27 巻 3 号 p. 151-156
    発行日: 1983/07/25
    公開日: 2009/03/31
    ジャーナル フリー
    抗CK-M血清を用い,CK-Bサブユニットを分離後生物発光法により測定するCK-MBアイソザイムの分析法を確立した.
    本法は酵素反応生成物であるATPの測定法に特徴を有しており,従来の最大発光強度を測定する方法や5~10秒間の発光量を積算する方法に対し,今回,最大発光(約5秒)に達する発光タイムコースに定量性を見出し,この間を積算(0.25秒)する新しい測定方式(初期積算法)を確立した.従って,測定には市販のホトメーターに新たにディレイ回路,積算回路および恒温セルホルダーを接続して用いた.
    本法を用いるとCK反応を停止させることなく,いわゆる1点速度法にて活性値を測定できる.測定装置がやや特殊ではあるが,高感度な方法であり測定精度もすぐれていた.また,電気泳動法との相関性もよく,今後,日常検査にも応用できる方法と思われる.
  • ゾーンの不変量による説明
    島尾 和男
    1983 年 27 巻 3 号 p. 157-163
    発行日: 1983/07/25
    公開日: 2009/03/31
    ジャーナル フリー
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