巻号一覧
後続誌
11 巻 (1965 - 1966)
- 4 号 p. 277-
- 3 号 p. 207-
- 2 号 p. 111-
- 1 号 p. 1-
11 巻, 4 号
選択された号の論文の5件中1～5を表示しています
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1966 年 11 巻 4 号 p. 277-326
発行日: 1966/07/10
公開日: 2009/03/31
ジャーナル フリーPDF形式でダウンロード (21890K) -
第2編 正常髄液蛋白質の分析麻上 義文1966 年 11 巻 4 号 p. 327-334
発行日: 1966/07/10
公開日: 2009/03/31
ジャーナル フリー1.同一髄液0.3,0.4,0.5,0.6mlを用いて同時に電気泳動し,使用髄液量と出現分画の数および濃度を検討した。その結果,髄液0.5mlを用いた場合が最もすぐれていると結論された。
2.同一泳動ゲルをくりかえし3回デンシトメトリーを行ない,デンシトメトリーの再現性を検討した。デンシトメトリーによる測定,成績の変動は比較的少なく,その再現性は満足すべきものであった。
3.正常血清と正常髄液の蛋白分画を比較検討し,髄液蛋白の由来は,血清成分の浸出の機転以外の機転も関与しているものと推定された。正常髄液17例の各蛋白分画の相対濃度百分率の平均値は,ρ:4.0%,A:73.7%,αL:2.1%,α1'～α2':3.8%,Tr:5.8%,β1':3.0%,βγ:5.6%,Sα2:2.0%を示した。抄録全体を表示PDF形式でダウンロード (6225K) -
小藤田 和郎, 岡崎 伸生, 村越 美砂子, 荒木 英爾1966 年 11 巻 4 号 p. 335-343
発行日: 1966/07/10
公開日: 2009/03/31
ジャーナル フリーA method is described for the determination of the fatty acid composition of serum cholesterol esters by gas liquid chromatography, after their separation by thin layer chromatography.
Serum extracts were prepared and washed by the method of Folch et al. Thin layer plates (20×20cm) were coated with Silica Gel G (Merk, Germany) to a thickness of 500μ.
A solution of total serum lipids in chloroform was applied linealy across the plate (2cm from the bottom). The plate was developed in a solvent system composed of petroleum ether (bp. 30-60°C)/diethylether, 95/5, v/v.
Thin layer chromatogram of total serum lipids showed widely separeted band of cholesterol esters (Rf, 0.9) and other three bands of triglyceride (Rf, 0.3) cholesterol (Rf, 0.2) and phospholipids (origin). The developing time was about 20 minutes. After chromatography, the band of cholesterol esters was scraped onto filter paper and eluted with 40ml of chloroform/methanol, 2/1, v/v.
An alkaline hydrolysis of cholesterol esters was performed with N/5-methanolic KOH for 2hr. and fatty acid was methylated by 10% BF3 methanol for 3 minutes. Methyl esters of fatty acid were chromatographed on a 25% ethylene glycol succinate polyester column at 198°C, using hydrogen flame ionization detector.
The minimum amount of cholesterol esters required for this method is about 4.0mg which correspond to 3ml of human serum. The following are average values of fatty acid composition of serum cholesterol esters from young healthy women (5 cases) analyzed by this method. Myristic acid: 0.8, palmitic acid: 14.5, palmitoleic acid: 4.6, stearic acid: 2.0, oleic acid: 20.4, linoleic acid: 52.1, and arachidonic acid: 5.6.
By the procedures described, cholesterol esters of serum lipids were quantitatively recoverd from thin layer plates and there were no detectable changes on their fatty acid compositions during thin layer chromatography.
Triplicate determination indicate that the method gives highly reproducible results.抄録全体を表示PDF形式でダウンロード (5422K) -
寒天電気泳動法によるLDH分画測定法吉田 光孝, 石川 和子, 北村 元仕1966 年 11 巻 4 号 p. 345-350
発行日: 1966/07/10
公開日: 2009/03/31
ジャーナル フリーThere have been difficulties in separating first and second fractions near the cathode in analysing serum lactate dehydrogenase (LDH) isozymes using conventional agar-gel electrophoretic methods. In order to obtain clear separation as well as reliable pattern of LDH isozymes, studies were performed by means of agar gel electrophoresis followed by the staining using nitroblue tetrazolium. The method for routine electrophoretic analysis of LDH isozymes for various body fluids or extracts was established.
1. Clear separation of LDH isozyme patterns could be obtained by improved cooling device as well as by the choice of proper electrophoretic and staining conditions.
2. It was found that the process for preparation of agar plate was important in order to obtain reproducible results.
3. Proper quantity of sample to use for LDH isozyme studies was examined using filter paper method by Ogita.
4. Ressler's staining condition was found to be most sensitive and reliable for our method.抄録全体を表示PDF形式でダウンロード (4019K) -
小川 恕人, 阿部 正和, 北村 元仕, 小酒井 望, 島尾 和男, 富田 仁, 平井 秀松, 門間 和夫1966 年 11 巻 4 号 p. 351-356
発行日: 1966/07/10
公開日: 2009/03/31
ジャーナル フリーPDF形式でダウンロード (2268K)
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