ウイルス
Online ISSN : 1884-3433
Print ISSN : 0042-6857
ISSN-L : 0042-6857
14 巻, 2 号
選択された号の論文の5件中1~5を表示しています
  • とくに非感染性腫瘍を中心として
    清水 武彦
    1964 年 14 巻 2 号 p. 55-59
    発行日: 1964/04/30
    公開日: 2010/03/16
    ジャーナル フリー
  • 松本 稔
    1964 年 14 巻 2 号 p. 61-67
    発行日: 1964/04/30
    公開日: 2010/03/16
    ジャーナル フリー
  • (II) キウリモザイクウイルス感染葉内におけるウイルス核酸ならびに蛋白質の出現時期
    三澤 正生, 加藤 盛
    1964 年 14 巻 2 号 p. 69-74
    発行日: 1964/04/30
    公開日: 2010/03/16
    ジャーナル フリー
    This report describes the process of formation of virus-RNA and virus-protein, and the changes of cyctoplasmic protein and nucleic acid metabolism in the tobacco leaves infected by cucumber mosaic virus.
    (1) In the CMV-infected leaves, 15 hours after inoculation, a considerable amount of free infectious RNA is synthesized at first and about 30 minutes later virus particles are formed. Namely, multiplication of the infectious RNA seems to precede the formation of viral-protein after infection.
    (2) The amount of free infectious RNA reached the maximum at about 18 hours after inoculation and decreased afterward as a consequence of combining with the protein. But the free infectious RNA was able to be detected slightly after 120 hours.
    (3) Soluble cytoplasmic proteins of the inoculated leaves were isolated by paper electrophoresis. Eight fractions were separated from the leaves 72 hours after inoculation, but seven fractions from the healthy leaves. This new component moved faster than others, and was infectious.
    (4) Nucleic acids of infected leaves were fractionated by ECTEOLA-cellulose column. Elution was carried out with 0.1M neutral phosphate buffer and NaCl solution in the range of 0.1-1.0M. Each fraction was measured for RNA concentration by the optical density at 200mμ. A new fraction which was not found in the control preparation was isolated from the inoculated leaves 2 days after inoculation. This fraction was eluted with 0.4M NaCl solution and not contained the viral-RNA. It is supposed that CMV-RNA was not eluted as a single fraction by this method.
  • 北脇 達雄
    1964 年 14 巻 2 号 p. 75-83
    発行日: 1964/04/30
    公開日: 2010/03/16
    ジャーナル フリー
    Various purification procedures, such as ammonium sulfate precipitation, differential centrifugation, DEAE cellulose column chromatography and sucrose density gradient centrifugation, were applied in the purification of measles virus hemagglutinin. By combination of these various purification procedures, a highly purified measles virus hemagglutinin preparation was obtained.
    The purified hemagglutinin showed antigenicity for hemagglutination inhibiting and neutralizing antibodies. This suggests that the protein coat of virus particles, split off by ether-tween 80 treatment, may have antigenicity both for hemagglutination inhibiting and neutralizing antibodies.
  • 俵 寿太郎
    1964 年 14 巻 2 号 p. 85-88
    発行日: 1964/04/30
    公開日: 2010/03/16
    ジャーナル フリー
    犬腎培養細胞に麻疹ウイルスEdmonston株を感染継代中であるが, 感染細胞にはエオジン好性の細胞質内及び核内封入体を認めることが出来た.
    これを連続切片法で電子顕微鏡的に観察して, 核内封入体部にみられるフィラメント様構造物は, 封入体形初期には不規則に配列するが, 後次第に凝集してきて束状となり, ついにはこのもので封入体全部を形成するに到るをものであることが判つた. これらのフィラメント様構造物は, 長いものでは450mμのものもみられ, 内径約10-15mμ, 外径約15-20mμの管状構造を有していた. そしてこのものとウイルス粒子との関係は, 未だ明かではないが, これは麻疹ウィルスによる感染の標識となるものであると考えられる.
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