The experimental results obtained in 4 field trials in 1964 of combined use of live and killed measles vaccines may lead to the following conclusion. For the mass vaccination againt measles, live vaccine inhalation after a single injection of potent killed vaccine seemed to be the ideal method for the “take” of live vaccine without clinical reactions.
A simple and sensitive isolation procedure for respiratory viruses and a rapid identification of these isolates were intended. RS virus was isolated more easily and rapidly by using mixed culture of HEp-2 and VERO cells rather than primary monkey kidney or human diploid cell cultures. By means of immunofluorescent staining, identification of RS virus was possible within 24 to 48hours postinoculation. Whereas, apparent CPE was not detectable until 6 to 9 days.