Infection experiments of measles virus (Edmonstone strain) and canine distemper virus (Hokkaido strain) in dog kidney cultured cells were conducted under the similar experimental conditions, and properties of these viruses were observed.
The formation of multinucleated giant cells and the formation of eosinophilic intranuclear and cytoplasmic inclusion bodies were observed in the infection by both viruses.
However, there was a considerable difference in the length of period required for the appearance of the CPE after the virus inoculation, namely, it took a short period with measles virus while a longer period with distemper virus.
As for the infectivity of these two viruses to dog kidney cultured cells both showed a stable infection titer on the second to the third generation in successive cultivation, but the measles virus titer was higher than that of the distemper virus.
Both viruses showed infectivity to HeLa cells, FL cells, PS cells and JTC-3 cells, and measles virus showed only a weak infectivity to L cells while the distemper virus failed to show any infectivity even in several infection attempts.
Serologically, the serum of measles patient neutralized measles virus and it more effectively neutralized distemper virus.

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Several viruses were examined for their interferon (IF) inducing capacity in chick embryo cell cultures. Newcastle disease virus (NDV) inactivated by UV-irradiation was found to be a capable inducer of IF production, whereas infectious NDV had no or undetectable IF-inducing capacity. Infectious Sindbis virus coud induce production of IF in chick embryo cells. Neither influenza virus (PR8) nor HVJ showed any detectable IF-inducing capacity even when they were inactivated by UV. From these results, a possible correlationship between IF-inducing capacity and growth characteristics of virus in chick embryo cells is discussed.

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Production of interferon (IF) in chick embryo cell cultures induced by UV-irradiated NDV (NDV_uv) or Sindbis virus was markedly suppressed by superinfection with active NDV or herpes simplex virus. Such inhibition of IF production was complete when the cells were infected with a large amount of NDV not later than 6 hours after the addition of NDV_uv. The effectiveness of superinfecting virus was much reduced if it was inoculated later than 6 hours after the addition of IF-inducer or if the multiplicity of infection was reduced to 0.01. When the cells were infected with NDV 20 hours after the inoculation of NDV_uv, production of IF was not reduced but rather enhanced. Neither influenza virus (PR8) nor HVJ showed such suppresseing effect on IF production induced by NDV_uv.

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A factor which inhibits interferon production was released into culture fluids of chick embryo cells infected with Newcastle disease virus (NDV). This factor could be detected by its ability to inhibit IF production induced by UV-irradiated NDV or Sindbis virus in chick embryo cell cultures. Inhibition of IF production was attained by preincubation of the cultures with this material for longer than 3 hours at 37°C before inoculation of IF inducer. This material appeared to be a nonviral cellular product formed along with the multiplication of NDV. We propose the term “Interferon Depressor (ID)” for this material. Interferon depressor was stable to heating at 90°C for 1 hour, nondialyzable, not sedimented by centrifugation at 60, 000g for 1 hour, nor neutralized by anti-NDV serum. Treatment with trypsin or DNase caused slight decrease in ID activity, whereas treatment with RNase enhanced ID activity.

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Infantile diarrhoea with white stool occurs every winter from November to March in Sendai. The virus isolation was attempted from 26 cases of this diseases occured from November 1965 to February 1966. Employing human embryonic kidney fibroblasts, cytomegalovirus (CMV) was isolated from the throat swabs of 6 cases of 17 (35.3%). Then CF antibody against the isolated strain of CMV in paired sera obtained from the patients was titrated. However, significant rise of CF antibody was demonstrated only in 2 cases of 19 and the CF antibody was detected even in the acute sera obtained from 10 cases of 19. The CF antibody against the same virus in sera obtained from the healthy infants was also titrated in the comparative manner. The CF antibody was found in 46.4% of them on summer 1965, in 35.3% on autumn before the occurrence of the disease, in 31.5% on winter during the epidemic of the disease and 47.4% on next spring after the epidemic, while demonstrated in 61.3% of the patients.
Thus CMV may be or may not be the etiological agent of this disease, although the seroepidemiological results that CMV seemed to be spread in the winter during the epidemic of the disease, taken together the high ratio of CMV isolation from the patients, suggested the posibility that CMV may be the cause of this disease.

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In spring of 1964, 3 non-polio virus strains were isolated from a paralytic patient of compatible case, which occured after administration of live polio virus vaccine and 5 strains were isolated from his healthy family.
From the results of the physical and biological examinations, these strains (named MT strains) are considered to be a member of ECHO virus group and they are neutralized by anti-MT-1 (representative strain of MT group virus) serum, but they are not neutralized by any previously described serotype except ECHO 4.
Furthermore, these strains posses high hemagglutinating activities with human O blood cells and show low grade one way cross reaction with ECHO 4 virus group, i.e. they are neutralized by anti Pesascek serum but anti-MT-1 serum dose not neutralize neither Pesascek strain nor Du Toit and other strains of ECHO 4.
As to the question whether these strains should be classified as a new type ECHO virus or intratypic variant of ECHO 4 group, we must continue further examination.

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大腸菌雄株で特異的に増殖するδAファージの精製と性質の検討を行なつた。
δAファージは形態的にはせんい状で, 直径約6mμ, 長さ約830mμの細長いファージで, 粒子量1.7×10⁷を示す。
ファージは46Sの沈降定数を示し, CsCl中で密度1.30となり, 分子量1.7×10⁶の一本鎖DNAを約10%含む。
DNAの塩基組成の検討を行なつたところ, アデニン:グアニン:シトシン:チミン=25.7:21.2:20.0:33.1であつた。
その結果, 形や大きさ, 核酸含量, CsCl中での密度塩基組成などの性質が今まで報告されたfd, f1, M13などと似かよつていることがわかる。

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