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Volume 10 (1969)
- Issue 6 Pages 365-
- Issue 5 Pages 299-
- Issue 4 Pages 243-
- Issue 3 Pages 147-
- Issue 2 Pages 59-
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Volume 10, Issue 3
Displaying 1-11 of 11 articles from this issue
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Tomotari MITSUOKA1969 Volume 10 Issue 3 Pages 147-156
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (1443K) -
1. Physico-chemical Properties of Addition Compounds (1)Hiromi NAKATANI, Kiyoshi KATAGIRI, Masaji MURAKAMI1969 Volume 10 Issue 3 Pages 157-161_1
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSStudies on the reactivity of various phosphates with hydrogen peroxide were carried out. Addition compounds of sodium phosphate dibasic, sodium pyrophosphate and sodium polyphosphate with hydrogen peroxide were obtained in crystalline powder through practical production method.
Physico-chemical properties of Na2HPO4·H2O2 and Na4P2O7·2H2O2 were investigated by means of thermogravimetric analysis, IR spectra and X-ray diffraction. It was confirmed that they were addition compounds of phosphates with hydrogen peroxide from these results.View full abstractDownload PDF (520K) -
II. Physico-chemical Properties of Addition Compounds (2)Hiromi NAKATANI, Kiyoshi KATAGIRI, Masaji MURAKAMI1969 Volume 10 Issue 3 Pages 162-166_1
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSInvestigations on the physico-chemical properties were carried out with addition compounds, Na2HPO4·H2O2 and Na4P2O7·2H2O2.
(1) From the results of polarography and paper chromatography, it was found that these addition compounds in aqueous solution were separated into corresponding phosphates and hydrogen peroxide, respectively.
(2) Addition compounds in crystalline form were fairly stable below 60°C and there was no detectable decomposition of the hydrogen peroxide.
However, the stability in aqueous solution was greatly affected by heating. And the decomposition of hydrogen peroxide was significantly accelerated by heating at high temperature. Especially, hydrogen peroxide of Na4P2O7·2H2O2 was more stable than that of Na2HPO4·H2O2.
(3) Addition compounds were found to show the hygroscopicity at relative humidity above 60%, and then the hydrogen peroxide was decomposed in accordance with increasing amount of equilibrium water. The stability of Na2HPO4·H2O2 was less than that of Na4P2O7·2H2O2.View full abstractDownload PDF (470K) -
III. On the Antimicrobial Action of Addition CompoundsTogo YAMANO, Hiromi NAKATANI, Kumiko JONO, Katuhiko TOKUDA, Masaji MUR ...1969 Volume 10 Issue 3 Pages 167-170_1
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSInvestigations on the antimicrobial action of addition compounds, Na2HPO4·H2O2 and Na4H2O7·2H2O2, were carried out in aqueous solution.
(1) The action of hydrogen peroxide on microorganisms was not affected by the phosphates. The antimicrobial action of addition compounds was found to be shown by hydrogen peroxide of these compounds, and there was no difference in the anticrobial activity between addition compounds and hydrogen peroxide at the same concentration as of H2O2.
(2) The preservative properties of the addition compounds on soybean curd were similar to those of hydrogen peroxide.View full abstractDownload PDF (422K) -
Toshiharu KAWABATA, Tsuneo KOZIMA, Yasuhiko SAKAGUCHI1969 Volume 10 Issue 3 Pages 171-185_1
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn order to get more simplified and rapid method for the identification of microorganisms, especially for Gram-negative rods in various sea foods, the method proposed by Corlett et al. was repeated in the present experiment using 39 reference cultures of several genera, and 754 cultures of the isolates from fish which had been identified according to the method by Kawabata et al.
Results obtained may be summarized as follows. In addition, some partial modifications on the replica plating method are being proposed:
Good coincidence was observed in the test organisms belonging to Pseudomonas group II, Flavobacterium and coliform, of both reference cultures and the isolates.
Several reference and isolated cultures belonging to Pseudomonas group I species, however, were identified as Pseudomonas group II according to the original method by Corlett et al. This was found to be resulted from the use of an improper basal medium (TPN) on which the test organisms failed to produce green fluorescent pigments. The difficulty or defect in the original method could be solved by using the medium B of King et al. instead of TPN.
We also encountered with difficulty in identifying genus Achromobacter; among the test organisms consisting of 7 reference cultures and 38 isolates of Achromobacter species, only 8 species could be identified as they are, and the remainders were unidentified according to the key proposed by Corlett et al. These results strongly suggest the need for further studies so as to solve the defect in identifying genus Achromobacter.View full abstractDownload PDF (1358K) -
2. Simultaneous Determination of Synthetic Preservatives by Use of Programmed Temperature Gas ChromatographyTadahiro NARAFU, Junko HAYAKAWA, Hiroaki TAKAHASHI, Yasuo ISHIDA, Taka ...1969 Volume 10 Issue 3 Pages 186-189_1
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSApplication of programmed temperature gas chromatography (GC) has been reported for fatty acids etc., but has not been used routinely in the determination of food additives.
A Shimadzu Model GC-1C gas chromatograph, equipped with a flame ionization detector and temperature-programming apparatus, was used in our investigation. Simultaneous gas chromatographic determination was tried on six synthetic preservatives. However, accurate quantitative determination of sorbic acid, dehydroacetic acid and benzoic acid cannot be expected because the retention times of the peaks of these preservatives were so early that these peaks showing might be masked in the peak of the solvent.
Thus simultaneous determination of preservatives both early and late retention times was possible only by use of the programmed temperature GC with FID.
It was known that the application of programmed temperature was not always followed with accurate results, because both values of the relative peak area and the relative factors were affected by the different programmed gradients of temperature as compared with the case of isothermal operation.
However, the values obtained by programmed temperature operation must not be theoretically different from those determined under isothermal conditions.
As the results, the calibration curves, obtained by peak area ratio of each preservative to internal standard, show linearity within a range from 10 to 70μg for SoA and from 2 to 8μg for the others.View full abstractDownload PDF (485K) -
3. Simultaneous Determination of Antioxidants by Use of Programmed Temperature Gas ChromatographyJunko HAYAKAWA, Tadahiro NARAFU, Hiroaki TAKAHASHI, Yasuo ISHIDA, Taka ...1969 Volume 10 Issue 3 Pages 190-193_1
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn the previous reports, we used programmed temperature gas chromatography and obtained satisfactory results on the simultaneous determination of synthetic preservatives.
The present investigation was undertaken in order to reconfirm whether this method could be applied to antioxidants as well as synthetic preservatives. A Shimadzu Model GC-1C gas chromatograph with a flame ionization detector and a temperature programmer was used for this experiment. Samples were BHT and BHA mixtures. First we wanted to find out the most suitable condition for the system when samples were analyzed simultaniously, and the next test was investigation on the recoveries of them from shortening oils added with these antioxidants.
We conclude from the experiment that BHT and BHA are determined simultaneously by programmed temperature gas chromatography on the peaks separated completely, without interference from synthetic preservatives, such as dehydroacetic acid or sorbic acid.View full abstractDownload PDF (383K) -
Reaction of Dehydroacetic Acid in Acid MediaShigeru GOTO, Yoshiyuki HIRAKAWA, Sadao IGUCHI1969 Volume 10 Issue 3 Pages 194-198_1
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDehydroacetic acid (DHA) has been observed to be readily converted into 2, 6-dimethyl-4-pyrone (DMP) in acid media where its preservative activity is remarkable. The electronic analog computer was used for the kinetic study of the conversions of DHA and 2, 6-dimethyl-4-pyrone-3-carboxylic acid (DMP-COOH) in acid media (pH 1-3). It was shown that a consecutive reaction involving diacetylacetone (DAA) as a most important intermediate must be considered for the above conversions. And also the presence of diacetylacetone carboxylic acid (DAA-COOH) as an another intermediate was presumed.View full abstractDownload PDF (551K) -
On the Application of the LAS Medium to Detection of Clostridium perfringensTadataka TANIGUTI, Buhei ZENITANI1969 Volume 10 Issue 3 Pages 199-204_1
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe LAS medium shown in Table 1 was prepared as a selective enrichment-medium for Clostridium perfringens by modifying the TSN agar. Then the LAS medium was applied to isolating Cl. perfringens from the 171 samples of fish and the 22 samples of the other sea-foods The results obtained from the experiments are as follows.
1) In the selective enrichment-culture of Cl. perfringens with the LAS medium, the presence of Cl. perfringens was judged by both blackening the medium and producing gas in the medium. Consequently this judgement in the LAS medium was more available than the judgement by only blackening in the TSN agar (Table 1).
2) Cl. perfringens identified by Benoki's criterion was isolated in the high percentage of 93 from the 213 cultures with the LAS medium in which the judgement of the presence of Cl. perfringens was positive. Likewise, as for the criteria in Bergey fs manual (key to the species of genus Clostridium) and of Strong et al., the isolation rates were 83 and 67 per cent respectively (Table 2).
3) There was a little difference between the specimens of the body surface and those of the alimentary canal, in selecting Cl. perfringens in the LAS 46°C-cultures, when the isolates from the cultures were identified by Benoki's criterion (Table 3).View full abstractDownload PDF (817K) -
Studies on Thin Layer Chromatography of EDTAMidori YAMAGATA, Ryuji AMANO, Tatsuo KONDO, Hiroya TANABE1969 Volume 10 Issue 3 Pages 205-208_1
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThin layer chromatography was applied for the simplified detection of ethylenediamine-tetraacetic acid (EDTA) in foods, and for the separation of EDTA from the interfering substances for its determination, such as α-amino acids.
Spotted sample on the thin layer prepared with cellulose powder (Avicel SF), was developed with a solvent system of n-butanol+glacial acetic acid+water (1:2:2v/v). After development, the thin layer plate was dried and sprayed with glacial acetic acid, cobaltous chloride and hydrogen peroxide. The Rf value of EDTA was about 0.7 and the limit of detection was about 1.5μg.
By use of this method EDTA could be separated from many kinds of α-amino acid, especially aspartic acid which interfered strongly EDTA detection.View full abstractDownload PDF (372K) -
Tetsuo INOUE, Masahiro IWAIDA, Tsuyako SHIROTORI1969 Volume 10 Issue 3 Pages 209-214
Published: June 05, 1969
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESS
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