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Volume 28 (1987)
- Issue 6 Pages 427-
- Issue 5 Pages 311-
- Issue 4 Pages 219-
- Issue 3 Pages 159-
- Issue 2 Pages 109-
- Issue 1 Pages 1-
Volume 28, Issue 2
Displaying 1-7 of 7 articles from this issue
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Shingo MIZUOCHI, Keiko YOSHIDA, Hirokazu OGIHARA, Misao HARUTA1987 Volume 28 Issue 2 Pages 109-114_1
Published: April 05, 1987
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe applicability of the IDF method for the detection of Staphylococcal thermonuclease (TNase) produced in cooked rice was examined. The lower limit of detection of TNase levels was 0.01μg/g in cooked rice by the IDF method. TNase was detectable at S. aureus population levels of 107/g, whereas enterotoxin was detected after 16-24 hours. The IDF method was modified by adding the sample to the Tris-buffered solution directly in the course of TNase extraction, and the supernatant was obtained by centrifugation; in addition, greater sensitivity could be obtained by increasing both the well size and the amount of the supernatant tested. The lower limit of detection of TNase levels was 0.005μg/g in cooked rice by the modified procedure.View full abstractDownload PDF (722K) -
Tadashi SAKAI, Kuraki KESAMARU, Deng-Fwu HWANG, Tamao NOGUCHI, Kanehis ...1987 Volume 28 Issue 2 Pages 115-118_1
Published: April 05, 1987
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA total of 105 specimens of the trumpet shell Charonia sauliae (“boshubora”) were collected from June 1984 through July 1985, at Kamae and Tsukumi in Oita Prefecture. Digestive glands were excised from these specimens and examined for toxicity (as tetrodotoxin; TTX).
1) The frequencies of occurrence of toxic specimens at Kamae and Tsukumi were 43 and 55%, respectively.
2) The average toxicity scores at Kamae and Tsukumi were 15 and 3.4MU/g digestive gland, the highest toxicity scores being 115 and 8.3MU/g digestive gland, respectively.
3) The responsible toxin was partially purified by active charcoal treatment, followed by chromatography on Bio-Gel P-2. Analyses by TLC, cellulose acetate membrane electrophoresis and high performance liquid chromatography showed that the toxin was composed of TTX and related substances.View full abstractDownload PDF (463K) -
Hiroyuki SUNAGAWA, Koichi TAKESHI, Kunio KAMEYAMA, Yoshiaki ANDO1987 Volume 28 Issue 2 Pages 119-124_1
Published: April 05, 1987
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSClostridium perfringens was detected in 322 (74.2%) fecal specimens heated at 75°C for 20min and in 76 (17.5%) fecal specimens heated at 100°C for 60min, out of 434 specimens from healthy persons in Hokkaido. Enterotoxigenic C. perfringens was found in only 20 (4.6%) of the fecal specimens. The enterotoxigenic strain was isolated from 12 fecal specimens heated at 75°C for 20min and from 9 fecal specimens heated at 100°C for 60min, although it was isolated from one specimen after both of the heat treatments. A total of 936 C. perfringens strains were isolated from fecal specimens heated at 75°C for 20min and 645 strains of the total produced a sufficient number of spores in DS medium. Of the total (645), only 23 strains (3.5%) were enterotoxigenic, of which 15 strains produced heat-sensitive spores (A-type) and the remaining 8 strains produced heat-resistant spores (K-type). On the other hand, a total of 149 C. perfringens strains were isolated from fecal specimens heated at 100°C for 60min and 104 strains of the total produced large numbers of spores in DS medium. Of the total (104), only 16 strains (15.4%) were enterotoxigenic and produced heat-resistant spores (K-type) only.View full abstractDownload PDF (710K) -
Tomoyuki MIYAZAKI, Tatsunori YAMAGISHI, Masao MATSUMOTO1987 Volume 28 Issue 2 Pages 125-129_1
Published: April 05, 1987
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method for the determination of Mirex (1, 1a, 2, 2, 3, 3a, 4, 5, 5a, 5b, 6-dodecachlorooctahydro-1, 3, 4-metheno-1H-cyclobuta [cd] pentalene) and 1, 2, 4, 5-tetrabromobenzene (TBB) in human milk samples was developed. The extracts from these samples with hexaneether-ethanol solvent were partitioned with hexane-acetonitrile, cleaned-up by Florisil column chromatography, and determined by selected ion monitoring (SIM). The SIM analyses were carried out under the following conditions: TBB, m/z 392, 394 on 2.5% Bentone 34+2.5% DC 200 (210°C), or 2% OV-1 (190°C) column; Mirex, m/z 272, 274 and 546 on 2% OV-1 column (240°C). Recoveries of TBB and Mirex added to cow's milk (10g) were as follows: TBB 79.4% (10ng added), 83.4% (100ng added); Mirex 74.6% (10ng added), 72.5% (100ng added).
Human milk samples were collected from women living in the vicinity of Tokyo Metropolitan area. The residue levels of Mirex and TBB in these samples were ca. 1ng/g and 3ng/g, respectively, on a milk fat basis.View full abstractDownload PDF (575K) -
Takao MITSUHASHI, Kazuhiko ADACHI, Yoshio KANEDA1987 Volume 28 Issue 2 Pages 130-135_1
Published: April 05, 1987
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple and sensitive method was developed for the quantitative determination of total bromine in cereals. 2-Bromo-3-pentanone derived from the reaction of bromine with 3-pentanone was quantitated by gas chromatography (GC) with an electron capture detector (ECD).
A 2g sample was ashed with 0.2g of anhydrous sodium carbonate and 5ml of alkaline monoethanolamine solution. The ached residue was dissolved in 10ml of 0.1N sodium hydroxide solution. After neutralization with 2N sulfuric acid, the solution was made up to 20ml with water. This diluted solution was used as the sample solution. Ten ml of the sample solution was transferred to a separatory funnel. One ml of 5% 3-pentanone solution, 1ml of 0.1M potassium permanganate solution and 1ml of 10N sulfuric acid were added successively. Bromide ion was oxidized and the liberated bromine was reacted quantitatively with 3-pentanone. The resulting 2-bromo-3-pentanone was extracted with 10ml of n-hexane and determined by ECD-GC.
The recovery of bromide added to rice and wheat flour was ca. 86%, and the detection limit was 0.05μg/g.View full abstractDownload PDF (630K) -
Takayuki NAKAHAMA, Goro URAKUBO1987 Volume 28 Issue 2 Pages 136-141_1
Published: April 05, 1987
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDegradation of diphenylnitrosamine (DPN), diethylnitrosamine (DEN), and t-butylethylnitrosamine (t-BEN) by several kinds of enteric bacteria was investigated and the following results were obtained.
1) The degradation rates of DPN and DEN by 5 kinds of enteric bacteria during a 24-hour incubation were 66-90% (control, 15%) and 4-16% (control, 2%), respectively.
2) In experiments with Pseudomonas aeruginosa under the same conditions, 85% (control, not degraded) of DPN and 18% (control, 8%) of DEN were degraded, but t-BEN was practically not degraded.
3) It was found that 84% (control, 8%) of DPN and 31% (control, 11%) of DEN were degraded by bacteria extracted from the cecal content of guinea pig, but t-BEN was not degraded under the same conditions.View full abstractDownload PDF (677K) -
Kazumichi TAMURA, Riichi SAKAZAKI, Shigehiko KURAMOCHI1987 Volume 28 Issue 2 Pages 142-145
Published: April 05, 1987
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (470K)
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