The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
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Volume 13, Issue 3
Displaying 1-6 of 6 articles from this issue
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  • KONSTITUTION DER POLYPEPTIDE UND PROTEOLYTISCHE FERMENTE
    YASUTARO MIYANOKI
    1931 Volume 13 Issue 3 Pages 389-421
    Published: 1931
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • THE FATE OF THE GLYOXALS IN THE ANIMAL BODY
    FUTOSHI SAKUMA
    1931 Volume 13 Issue 3 Pages 423-440
    Published: 1931
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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    1. The susceptibility of the glyoxals to hydrolysis by aldali and glyoxalase was studied. They were. arranged in the order of sensibility, methyiglyoxal, phenylglyoxal, hydroxymethylglyoxal, glucosone and glyoxal.
    2. The lethal doses of the glyoxals for the rabbit were determined by injecting them subcutaneously and intravenously.
    3. The fate of the glyoxals in the animal body was examined by analysing the blood and urine for their content of glyoxals, lactic acid and sugar. The finding that the injected glyoxals existed in the blood for a much longer time than was hitherto supposed, was a rather unexpected result, in view of their reactivity and of their susceptibility towards glyoxalase.
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  • A COLD-HEMOTOXIN, NEWLY DISCOVERED IN THE HEATED SERUM
    Part II. The Cold. Non-Complementary Hemolysis by the Heated Normal Serum
    KATSUO TOSHIMA
    1931 Volume 13 Issue 3 Pages 441-464
    Published: 1931
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • ÜBER DEN EINFLUSS DER GALLENSÄURE AUF DEN CALCIUMSTOFFWECHSEL. (IV)
    Calciumausscheidung im Kot bei Zufuhr von Cholsäure
    KANZI FUZIWARA
    1931 Volume 13 Issue 3 Pages 465-471
    Published: 1931
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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    1. Die Kalkausscheidung im Kot ist von der Art der Nahrung abhängig.
    2. Die Kalkausscheidung im Kot wird derch die Zufuhr von Cholsäure sowohl der absoluten Menge nach als auch prozentual herabgesetzt.
    Aus den Daten geht hervor, dass die Zufuhr von Cholsäure das Calcium mehr im Harn und in der Galle, aber weniger im Kot zur Ausscheidung gelangen lässt.
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  • BIOCHEMICAL INVESTIGATION OF MOSAIC IN SOLANUM TUBEROSUM
    R. C. MALHOTRA
    1931 Volume 13 Issue 3 Pages 473-487
    Published: 1931
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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    1. Biochemical study of healthy and mosaic tomato plants has been undertaken.
    2. Fresh and dry weights of the plants were taken. Ether extract (oils, chlorophyll), alcohol extract (reducing sugars), non-reducing sugars, starch, hemicellulose. total carbohydrates, nitrogen, ash and calorific heat were determined quantitatively.
    3. The data seem to indicate that healthy plants made better growth both on fresh and dry weight basis. However, the percentage of dry matter was higher for the mosaic plants.
    4. Percentages of ether extract, non-reducing sugars, starch, hemicelluloses and calories of heat per gram were higher for healthy plants; while nitrogen, inert materials, including cellulose, were higher in deseased plants. Percentages of ash and reducing sugars was practically alike in both kinds of plants.
    5. Some suggestions for such biochemical results as shown by these plants have been made.
    6. There is a need for more thorough study in this connection which might elucidate the causal agency or the mechanism of mosaic diseases.
    It is a pleasure to acknowledge the co-operation shown by Mr. Ray Glynn, M. Sc., Research Assistant, Biology Department of this institution.
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  • A COLD-HEMOTOXIN, NEWLY DISCOVERED IN THE HEATED SERUM
    Part III. Experiments on its Chemical Nature and on the Influence of Substances Inhibiting other Sorts of Hemolysis
    KATUO TOSHIMA
    1931 Volume 13 Issue 3 Pages 489-511
    Published: 1931
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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    1. The substance causing the cold-hemolysis in the heated normal serum can not be salted out by means of SO4(NH4)2.
    2. It can not be extracted with lipoid-dissolving solvants such as alcohol, aceton, ether, chloroform, etc..
    3. It is always precipitated with protein when the serum is freed from protein by various methods.
    4. It can be entirely adsorbed by kailin and partly adsorbed by cholesterin.
    5. It is not dialysable.
    6. The non-protein colloids such as glycogen, metal colloid and silicic acid, etc. have nothing to do with the cold-hemolysis.
    7. Its adsorption by erythrocytes is very characteristic, that is, the erythrocytes adsorb it only when it. is in the active state in the heated serum, while the erythrocytes can not adsorb it. from the fresh unheated serum.
    8. The- cold-hemolysis is markedly inhibited by the fresh unheated sera of various animals (rabbit, guinea-pig, horse, chicken, goose and man).
    9. The serum-albumin can inhibit the cold-hemolysis in the same way as serum itself but the serum globulin is not endowed with such a power.
    10. The cold-hemolysis is remarkably inhibited by cholesterin, but not by lecithin
    . 11. It is also inhibited by egg yolk, but not by egg white.
    12. The cold-hemolysis is inhibited by various proteins such as pepton, casein and trypsin, but not by ovalbumin and pepsin.
    13. The bile of cattle and pig as well as the sodium salts of glycocholic- and taurocholic acid have no influence upon the cold-hemolysis.
    I wish to express my indebtedness to Prof. K. Yamakami for his kind direction and encouragement during my experiments.
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