The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Search
Advanced search
OR
Browse
Search
Volume 40, Issue 5
Displaying 1-14 of 14 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
  • CREATININE FORMATION FROM CREATINE BY YEAST
    TOKURO SODA, AKIRA YOSHIDA, ATSUSHI OIKAWA
    1953Volume 40Issue 5 Pages 421-426
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    1. It was found that, by baker's yeast and Fleischmann's yeast, creatine in media was converted to creatinine accompanied by the fermentation of glucose, but to far less degree by the respiration.
    2. Creatinine was not formed during the respiration of alcohol by yeast.
    3. NaN3 completely inhibited the creatinine formation indicating that the process may have some correlation with the phosphorylation.
    4. The amount of creatinine formed was nearly proportional to the amount of glucose and creatine present.
    It will be interesting to investigate whether creatinine in animal urine may be formed by the similar process in animal body.
    View full abstract
    Download PDF (363K)
  • ANTI-THIAMINE FACTORS OF THE FERN
    MOTONORI FUJIWARA, KIYOO MATUI
    1953Volume 40Issue 5 Pages 427-434
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Download PDF (388K)
  • THE METABOLISM OF CHOLIC ACID BY MICROORGANISM
    MASASHI MACHIDA
    1953Volume 40Issue 5 Pages 435-437
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Mori (3) had confirmed in the culture medium of B. subitlis that only a ketone group of the 7-position of dehydrocholic acid was reduced to an alcohol group. Shibuya (4), Yamasaki and Kyogoku (5) reported that if dehydrocholic acid was injected into a toad, 3(β)-hydroxy-7, 12-diketocholanie acid was excreted in its urine. It seems probable, therefore, that only ketone group of the 3-position of the cholane nucleus could be converted to alcohol group in the toad body.
    Takemoto (6) had proved that the ketone group of the 7-position of the bile acid nucleus was reduced to alcohol group and then to methylene group in putrefied media. The author found that ketone groups of the 3- and 7-positions of dehydrocholic acid were reduced into 3, 7-dihydroxy-12-ketocholanic acid by B. coli. These observations indicate that among the ketone groups of 3-, 7-, and 12-positions of bile acid nucleus most vulnerable to the reducing action by B. coli is the one at 3-position and then that at 7-position, while the ketone group at a 12-position has strong resistance towards the bacterial action. Note-worthy is the fact that the ketone group at 3-position is reduced only to α-form by the action of bacteria.
    View full abstract
    Download PDF (190K)
  • THE NITRATE UTILIZATION IN SEED EMBRYOS OF VIGNA SESQUIPEDALIS
    HISAKO KUMADA
    1953Volume 40Issue 5 Pages 439-450
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The nitrate absorption and the nitrate assimilation by excised organs of seed embryos of Vigna sesquipedalis grown in the dark were investi-gated. The most vigorous absorption was found in radicle, especially aged 4 days when lateral roots appeared. As for nitrate assimilation in the dark, plumule and radicle showed strong activity, and hypocotyl relatively weak one. The nitrate assimilation in these organs kept pace with the oxygen respiration, and under anaerobic conditions the as-similation was greatly diminished.
    In cotyledon absorbed nitrate was converted into nitrite, but not further. The nitrite production decreased as the age of tissue progressed, when a greater part of the nitrite accumulated in the tissue was put out into medium. In the early stage of germination the cotyledon tissue was found to have cytochromes of coli-type (a1, b), and in the later stage that of subtilis-type (a, b, c). A possiblity was pointed out that nitrate may serve as a terminal hydrogen-acceptor in the cotyledon tissue at least in the earlier stage of germination.
    The author wishes to express her gratitude to Assist. Prof. Dr. Yukito Oota under whose cordial guidance the present investigation was carried out. Thanks are also due to Prof. Dr. Takeshi Mori who has made valuable counsels and encouragement during the course of this study.
    View full abstract
    Download PDF (1113K)
  • STUDIES ON SOME NEW METABOLIC PRODUCTS OF PENICILLIUM. II
    HIROHISA MUNEKATA
    1953Volume 40Issue 5 Pages 451-460
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Download PDF (695K)
  • THIOL CONTENT AND SUCCINOXIDASE ACTIVITY OF ISOLATED RAT LIVER MITOCHONDRIA
    JUNKICHIRO HIRADE, TETSUO HAYASHI
    1953Volume 40Issue 5 Pages 461-469
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    1. In an attempt to follow out the correlation between the -SH contents and enzymatic activities studies were make on the effect of three main -SH reagents upon succinoxidase and α-ketoglutaric oxidase of isolated rat liver mitochondria as well as the reactivation experiments with GSH.
    2. Mercaptide-forming agents such as PCMB, HgC12 and CuSO4 diminished the -SH content in exact agreement with the inhibition of the enzyme activities of both types (Figs. 1, 4). The addition of GSH restored not solely the succinoxidase activity, but also proportionately the -SH content of the protein.
    3. Oxidizing agents such as tetrathionate and chloropicrin caused a reversible inhibition of succinoxidase activity, -SH groups disappearing at equal rate with enzyme activity, while both were restored to the same extent by the effect of GSH.
    4. Zn++, Cd++, iodoacetamide and ferricyanide showed poor inhibition of enzyme activity and low affinity toward -SH groups of the isolated mitochondria (Figs. 1 and 2, Table I). Chlorovinyl-methyl-ketone showed relatively high affinity.
    View full abstract
    Download PDF (514K)
  • ENZYMATIC TRANSFER OF β-D-GALACTOSE
    KATSUO TAKANO, TOMOO MIWA
    1953Volume 40Issue 5 Pages 471-476
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    1. By the action of enzymes of apricot emulsin and of green leaves of elder tree (Sambucus Sieboldiana) β-D-galactose residue of aryl-β-D-galactoside is transferred to alcohol forming alkyl-β-D-galactoside.
    2. The association of hydrolyzing and trasferring activities of apricot emulsin has been considered as suggesting the identity of hydrolase and transferase, where water and alcohol act respectively as the acceptor of β-D-galactose residue.
    3. The possibility of the existence of a variety of β-galactosidases with different affinities toward water and alcohols has been discussed.
    This investigation was supported in part by the Grant in Aid for Fundamental Scientific Research from the Ministry of Education.
    View full abstract
    Download PDF (376K)
  • BILE ACIDS AND STEROIDS I
    STUDIES ON HOG BILE ACIDS (PART 1). NEW SYNTHETIC ROUTE OF METHYL Δ4-3-KETOCHOLENATE FROM α-HYODESOXYCHOLIC ACIDS
    KEN'ICHI TAKEDA, JUN'ICHI KAWANAMI
    1953Volume 40Issue 5 Pages 477-483
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    An improved method for the conversion of α-hyodesoxycholic acid into 3-keto-6-α-hydroxycholanic acid has been found in the oxidation of the free acid or ester at C3 with N-bromosuccinimide in hydrated acetone solution in a quantitative yield. The oxidation agent acts selectively and the hydroxyl group at C6 remains unattacked. And 3-keto-6-hydroxy acid is converted easily into Δ4-3-keto acid by the usual method.
    We express our sincere gratitude to Prof. Dr. Yamasaki, Tottori University, Yonago, who generously sent us the sample of 3-hydroxy-6-ketoallocholanic acid. The author thanks Mr. T. Ieki and Mr. K. Miyahara for microanalyses, and Messrs T. Kubota and Y. Matsui for the determination of ultraviolet absorption spectra.
    View full abstract
    Download PDF (345K)
  • THE STRUCTURE OF ADENYLTHIOMETHYLPENTOSE. I
    KIYOO SATOH
    1953Volume 40Issue 5 Pages 485-492
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Download PDF (473K)
  • ON THE CHEMICAL NATURE OF BLOOD COAGU-LATION ACCELERATORS CONTAINED IN THE BONE MARROW EXTRACT
    HONG RIH-CHING
    1953Volume 40Issue 5 Pages 493-500
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The author was able to separate hypoxanthine, inosine and guano-sine as the active principles from the bone marrow extract which showed a remarkable accelerating action on the blood coagulation by paper chromatography. This was done by cutting the whole filter paper in several sections separately along the Rf values of the detected spots, eluting them with water, and investigating their physiological action. Then the chemical nature of the substances which were contained in the elutes from the A and B fractions having accelerating action on the blood coagulation was investigated with various tests.
    The author would like to extend his appreciation to Prof. Katashi Makino for his kind guidance and also to Dr. Kiyoo Satoh for his cooperation in reading the ultraviolet absorption spectra.
    View full abstract
    Download PDF (482K)
  • ON THE CHEMICAL NATURE OF BLOOD COAGULA-TION ACCELERATORS CONTAINED IN THE SPLEEN EXTRACT
    HONG RIH-CHING
    1953Volume 40Issue 5 Pages 501-507
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The author was able to separate hypoxanthine and inosine as the blood coagulation accelerators from the spleen extract by paper chroma-tography and identified by the paperchromatographic and ultraviolet spectrophotometric technics. Moreover, the author described the remarkable accelerating action on the blood coagulation of pure hypoxanthine, inosine and guanosine and recognized that they are ef-fective only in vivo but not in vitro.
    The author would like to extend his appreciation to Prof. Katashi Makino for his kind guidance and also to Dr. Kiyoo Satoh for his cooperation in reading the ultraviolet absorption spectra.
    View full abstract
    Download PDF (401K)
  • STUDIES ON THE ENZYMATIC BREAKDOWN OF LIMITDEXTRIN
    I. THE ENZYMATIC BREAKDOWN OF β-LIMITDEXTRIN BY α-AMYLASE
    MAKOTO SEIJI
    1953Volume 40Issue 5 Pages 509-514
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    β-Limitdextrin was prepared from amylopectin by the action of barely β-amylase. The action of salivary α-amylase on β-limitdextrin was examined by the iodine potentiometric titration method. The titration curves showed to be of the same type as that of amylopectin. It was demonstrated that the salivary α-amylase split the internal α-1, 4-linkages in amylopectin and β-limitdextrin in the same way in-dependently of the α-1, 6-linkage of all end chaine.
    The author wishes to express his gratitude to Prof. K. Kodama and Prof. H. Yoshikawa for their reading of the manuscript and kind advices.
    The research was supported in part by the Research Expenditure of the Ministry of Education.
    View full abstract
    Download PDF (311K)
  • STUDIES ON THE ENZUYMATIC BREAKDOWN OF LIMITDEXTRIN
    II. THE α-LIMITDEXTRIN
    MAKOTO SEIJI
    1953Volume 40Issue 5 Pages 515-518
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    α-Limitdextrin was prepared from amylopectin by the action of α-amylase. Its properties were studied.
    1. α-Limitdextrin was stable against α-amylase for 48 hours.
    2. The ratio of the rate constant of acid hydrolysis of α-limit-dextrin to that of amylose was about 1:3.
    3. It was recognized by paper chromatography that α-limit-dextrin consisted of two or three kinds of reducing sugars. These results indicate that the α-Limitdextrin obtained by the author contains the compounds consisting mainly of α-1, 6-linkages.
    The author wishes to express his gratitude to Prof. K. Kodama and Prof. H. Yoshikawa for their reading of the manuscript and kind advices. His thanks are also due to Mr. M. Hosoya for his valuable technical assistance in this study.
    The research was supported in part by the Researdh Expenditure of the Minis-try of Education.
    View full abstract
    Download PDF (182K)
  • STUDIES ON DIGESTION OF STARCH BY α-LIMITDEXTRINASE
    MAKOTO SEIJI
    1953Volume 40Issue 5 Pages 519-525
    Published: 1953
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Download PDF (411K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top