The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Volume 23, Issue 3
Displaying 1-10 of 10 articles from this issue
  • Studies on the blood pH estimated by the glass electrode method. V
    HISATO YOSHIMURA
    1936Volume 23Issue 3 Pages 335-350
    Published: 1936
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    1. A new micro-lass electrode for 0.07cc. material was designed. Method of the pH determination with it was described in details.
    2. This micro-electrode was applied to the measurement of the blood pH, and it was found that it gave a correct value within 0.02 pH.
    3. In experiments on rabbits loosely fixed on a board and on resting human adults, it was confirmed by direct measurement that the pH of arterial blood is more alkaline by 0.03 pH on an average than that of venous blood, while the former coincides with the pH of cutaneous capillary blood within the scope of experimental error.
    The expenses of this research were defrayed by a grant from the Hattori-Hoko-Kwai. to whom my thanks are due.
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  • I. Hydrolysis of Purine Ribonucleotides
    H. ISHIKAWA, Y. KOMITA
    1936Volume 23Issue 3 Pages 351-363
    Published: 1936
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • XIV. A few modifications of the Zuckerkandl and Messiner-Klebermass method for determination of glucosamine and distribution of glucosaminase among bull tissues
    KUNIICHI WATANABE
    1936Volume 23Issue 3 Pages 365-369
    Published: 1936
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • XV. A modified Zuckerkandl and Messiner-Klebermass method for determination of N-mono-acetylglucosamine and its application to biochemical analysis
    KUNIICHI WATANABE
    1936Volume 23Issue 3 Pages 371-376
    Published: 1936
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • Über das Reagens einer Fällungsreaktion des Lepraserums
    IHOE SAKAKIBARA
    1936Volume 23Issue 3 Pages 377-420
    Published: 1936
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • KAZUO TSUZUKI
    1936Volume 23Issue 3 Pages 421-435
    Published: 1936
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    Each phase of the reactions involved in the Embden-Meyerhof theory on the production of lactic acid from hexosedi-phosphoric acid by muscle tissue, was investigated with the employ-ment of Kato spindle cell sarcoma of rabbit instead of muscle. The results obtained are as follows.
    1. Triosephosphoric acid was produced from hexosediphos-phoric acid by tumour tissue. The velocity of the reaction was. however, slower than that of muscle tissue.
    2. Tumour tissue was unable to dismutate two molecules of triosephosphoric acid into phosphoglyceric and glyceroiphosphorie acids.
    3. The decomposition of phosphoglyceric acid into pyruvic acid was not carried out by tumour tissue, the reaction being stopped at the stage of phosphopyruvic acid. However, pyruvic acid was easily produced from phosphopyruvic acid in the presence of adenosinetriphosphoric acid and adenylicc acid, or phosphatase.
    4. The dismutation of pyruvic acid into lactic acid in the presence of α-glycerolphosphoric acid was not observable in tumour tissue.
    5. In spite of the facts mentioned above that the process of the glycolysis of the Embden-Meyerhof hypothesis was stopped at several phases, a small amount of lactic acid was always produced from hexosediphosphoric acid by the tumour slices. The mechanism of this reaction is quite obscure at present. This reaction was inhibited only to a relatively slight degree by the quantities of fluoride or monoiodoacetic acid which completely stopped the production of lactic acid from glucose.
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  • Untersuchungen über Kephalin als Reagens der Fällungs-reaktion des Lepraserums
    IHOE SAKAKIBARA
    1936Volume 23Issue 3 Pages 437-448
    Published: 1936
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • I. A. SMORODINTSEV, P. A. TSYGANOVA
    1936Volume 23Issue 3 Pages 449-452
    Published: 1936
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • I. A. SMORODINTSEV, N. N. KRYLOVA
    1936Volume 23Issue 3 Pages 453-454
    Published: 1936
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
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  • II. The action of the potato tyrosinase on various phenolic substances and the effect of addition of various amino compounds on that tyrosinase system
    FUJIO NOBUTANI
    1936Volume 23Issue 3 Pages 455-485
    Published: 1936
    Released on J-STAGE: November 18, 2008
    JOURNAL FREE ACCESS
    1. In this paper the comparison was made of the action of tyrosinase on various phenolic substances alone or in the presence of a number of amino acids and other amino compounds.
    2. Only those phenols, which are likely to produce ortho-quinones on oxydation gave coloured substances and the presence of amino acids markedly accelerated the production of pigments.
    3. The oxygen up-take per molecule of p-cresol or catechol by the action of tyrosinase was found to be one atom more than that corresponding to the formation of ortho-quinone.
    4. The presence of amino acids and a series of aromatic amino compounds remarkably accelerated the rate of oxygen consumption. The total amount of oxygen absorbed however was not in any way affected by the addition of amino compounds.
    5. The suggestion was made that amino acids combine with orthoquinones produced by the tyrosinase action.
    6. Hydroxylamine accelerated the colour development of the tyrosinase system. The oxygen consumption was on the contrary depressed. The discussion was made on the probable cause of this phenomena.
    In conclusion the auther wishes to express his sincere thanks to Prof. K. Kodama and Prof. M. Shiraki for their continual interest and much valuable advice during this work, to Assistant Prof. T. Uchino for his kind direction through-out this work.
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