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Volume 44, Issue 4
Displaying 1-7 of 7 articles from this issue
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II. UPTAKE OF RADIOACTIVE CALCIUM FROM THE DIET BY INCISOR TOOTH AND BONE OF RATYOSOJI ITO, SUSUMU TSURUFUJI, EICHI MURAI1957 Volume 44 Issue 4 Pages 195-203
Published: April 25, 1957
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSGroups of litter mates of growing male albino rats were placed on an adequate synthetic diet and a calcium balance experiment was car-ried out, not only by conventional means, but also with the aid of radio-active calcium. After sacrifice of the rats, their lower incisor teeth and femoral bones were removed and analysed.
Correlation coefficients among food intake, gain in body weight and intake, excretion and retention of ordinary and radioactive calcium were calculated.
Conclusions drawn from this experiment are as follows;
1. Bony skeleton of growing rats takes more radioactive calcium from the diet than expected from the increase of skeletal calcium by bone growth, while continuously growing incisor tooth takes less Ca45 than expected from the rate of incisor growth.
These facts may indicate a difference in the mechanism of radio-active calcium incorporation between bony skeleton and continuously growing incisor in a growing rat.
Radioactive calcium may enter the skeletal tissue by at least two principal processes, physiological tissue formation and physicochemical exchange. On the contrary, it is supposed that incisor tooth of the rat which is growing actively receives radioactive calcium mainly by physiological tissue formation.
2. While the rate of growth of animals is parallel with their food ingestion, skeletal growth is not so closely related to calcium intake
(nor to food intake) as body growth. A larger intake of calcium causes instead a larger excretion of calcium than retention.
3. Little correlation between incisor and femur in the uptake of radioactive calcium suggests a difference in the mode of radiocalcium uptake.View full abstractDownload PDF (538K) -
II. ELECTROPHORETIC STUDIES ON PHOSVITIN, LIPOVITELLIN, AND LIPOVITELLENINHIROSHI SUGANO1957 Volume 44 Issue 4 Pages 205-215
Published: April 25, 1957
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESS1. Concerning the nature of phosvitin and its isolation process, some investigations were carried out by using an electrophoretic method. The mobility of phosvitin was -11.3 units at pH 9.8 and μ0.15. The phosvitin represented an average of 8.5 per cent of the yolk protein. The lipid and phosphorus contents were in good agreement with the results of Mecham and Olcott. The solubility property of the isolated phosvitin was stated.
2. The homogeneity of the lipovitellin prepared by the method of Alderton and Fevold was studied electrophoretically. This preparation was not homogeneous and its protein composition was given. Two provisional names, “α-lipovitellin” and “β-lipovitellin”, were used for two main electrophoretic components of the yolk protein, which had the mobilities of -5.3 and -4.4 units, respectively. About 72 per cent of the total phosphorus and about 83 per cent of the protein phosphorus of the lipovitellin were attributed to the 16 to 18 per cent phosvitin which was contained in it. And similarly, about 81 per cent of the lipid of the lipovitellin was accounted for β-lipovitellin which was involved 26 to 29 per cent in it.
3. The preliminary study on the isolation of a-lipovitellin from the lipovitellin was reported. The isolated a-lipovitellin preparation showed the purity over 90 per cent and it contained 0.7 to 0.8 per cent phosphorus and 6.5 to 7.0 per cent lipid. The solubility property of this preparation was stated.
4. The lipovitellenin prepared by the method of Fevold and Lausten was studied by the electrophoretic method. As compared with the lipovitellin, this preparation was considerably pure; namely, over 85 per cent of it consisted of the β-lipovitellin and the phosvitin was not contained. About 83 per cent of phosphorus of the lipovitellenin was due to the phospholipid which was alcohol-extractable.
The author wishes to express his thanks to Mr. K. Terao and Miss. I. Watanabeor their technical assistances in carrying out the experiments.View full abstractDownload PDF (620K) -
KIKUO ARAKAWA1957 Volume 44 Issue 4 Pages 217-225
Published: April 25, 1957
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESS1. Many non-hydroxyamino acids were oxidized by neutral periodate at 60° for 24 hours to be converted into NH3, CO2, and their respective corresponding aldehyde, but not so at any appreciable rate at room temperature.
2. The oxidation velocities of a variety of non-hydroxyamino acids were determined until 24th hour and compared with one another.
3. The oxidation patterns of some amino acids were discussed.
The author wishes to express his sincere gratitude to Prof. Dr. R. Hirohata, director of the Department, and Prof. Dr. M. Tomoda, director of the Department of 2nd Surgical Clinic, for their careful guidance given throughout the present re-search.View full abstractDownload PDF (461K) -
ZIRO NIKUNI, ROY L. WHISTLER1957 Volume 44 Issue 4 Pages 227-231
Published: April 25, 1957
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSElectron microscopic observation of ultra-thin sections of certain corn starch granules shows fissures and borings which are similar to those produced in methacrylate impregnated sections treated with salivary amylase.View full abstractDownload PDF (1620K) -
VI. SEPARATION OF HYDROGENASE AND THIOSULFATE REDUCTASE AND PARTIAL PURIFICATION OF CYTO-CHROME AND GREEN PIGMENTMAKOTO ISHIMOTO, JIRO KOYAMA1957 Volume 44 Issue 4 Pages 233-242
Published: April 25, 1957
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSExtracts of sulfate-reducing bacteria were fractionated with ammonium sulfate or acetone and hydrogenase, thiosulfate reductase, cytochrome and green pigment were partially purified for the purpose of separation. Some properties of these enzymes and pigments are reported. Reconstruction of thiosulfate reducing systems was carried out successfully with hydrogenase and thiosulfate reductase preparations.
The authors wish to thank Dr. T. Soda and Dr. S. Akabori for their kind guid-ance and Dr. H. Tamiya for his valuable advices throughout this investigation. The expences for the work were aided partly by the Scientific Research Funds from the Ministry of Education.View full abstractDownload PDF (509K) -
I. ON THE INORGANIC COMPOSITIONYOSHIO OKUDA1957 Volume 44 Issue 4 Pages 243-248
Published: April 25, 1957
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESS1. The present paper is a biochemical study of the inorganic composition of the fresh-water medusa Craspedacusta sowerbyi Lankester, with special reference to spectrographic and chemical methods of analysis.
2. I have found, as the inorganic components in the fresh-water medusa, sodium, potassium, calcium, magnesium, chlorine, phosphorus, silicon, iron, copper which were also recognized in the marine medusa Aurelia aurita.
3. It is a notable fact that iron and copper are present in this animal.
4. The ratio of potassium to sodium in C. sowerbyi is much less than the ratio of these two elements in fresh water.
5. The inorganic endowment of C. sowerbyi was sodium 25.82mg., potassium 12.30mg., magnesium trace, calcium 5.56mg., chlorine 46.78mg. in 1g. of dried material.
6. The salinity of this medusa is about 3 persent of that of ocean water though it is more than 15 times of that of river water.View full abstractDownload PDF (903K) -
BROMINATION OF 7-KETOCHOLANIC ACID DERIVATIVEKEN'ICHI TAKEDA, TAICHIRO KOMENO1957 Volume 44 Issue 4 Pages 249-251
Published: April 25, 1957
Released on J-STAGE: November 18, 2008
JOURNAL FREE ACCESSDownload PDF (147K)
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