1. The content of H₂-donator (a sort of hexosephosphate) of the B-avitaminised fowls was found to be much reduced in compariGon with those under normal conditions or acute inanition by testing with the methy lenblue technique of Thunberg.
2. The candiolin-dehydrogenase activity of the heart muscle of vitamin-B deficient fowls was also reduced highly.
3. On vitamin-B deficient diet, the hypertrophy of suprarenal cortex accompanied with the accumulation of the coferment for candiolindehydrogenase was observed.
4. The application of vitamin-B preparation to the avita-minised fowls brought about a prompt restoration to the normal state in every respect of the above disturbances, unless the animal was too much exhausted.
5. The addition of suprarenal cortex to the vitamin-B deficient diet delayed the onset of avitaminose, which effect was not observed with other tissues, such as liver, kidney and brain.
The author is deeply indebted to Professor K. Kodama and to Professor R. Kaneko for their kind advice throughout this investigation.

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The fact that lactodehydrogenase in the heart muscle of fowls fed on polished rice is severely injured, is proved methylenblue technique and redox potential measurement.
The author is deeply indebted to Professor K. Kodama to Professor R. Kaneko for their kind advice throughout this investigation.

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Chondroitinsulfuric acid can be estimated quantitatively in such a small quantity of cartilage as 5 mgm. of the dry powder by means of the Zuckerkandl-Messiner-Klebermass method.
The writer is indebted to Professor K. Kodama for criticisms, to Dr. I. Kubo, Professor of Otorhinolaryngology for continuous encouragement, and to Assistant Professor H. Masamune for kind direction in the course of this work. The writer acknowledges gratefully also the kindness of Dr. M. Moriyama in the analysis of glucuronic acid.

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1. The chondroitinsulpf uric acid of cartilage was estimated and calculated in percentages of dry powder. The results may be summarized as follows:
a) The content of the chondroitinsulfuric acid varies according to the sort of cartilage ; namely, the hyaline cartilage of the grown-up but not old animal contains the biggest, the fibrocartilage the smallest amount and the elastic cartilage lies between these two sorts.
The costal cartilage makes an exception, because of early commencement of ossification in it.
b) The soft part of the hyaline cartilage is richer in the acid than the hard ossifying part is.
1) The colour depth corresponded to about_??_times that developed by 1mg chondrosamine chlorhydrate.
c) The acid of the hyaline cartilage increases with age; in other words, with the ossification.
2. The acid in question appears in bone in rickets in accord with the process of chondrification.
3. The reduction method may be made use of for measurement of the chondroitinsulfuric acid in cartilage, when not so exact a value is desired. The amount of the dry cartilage powder necessary for this purpose is as small as 1mgm.
In conclusion, I should like to express my best thanks to Professor K. Kodama for his helpful advice and criticism; to Dr. I. Kubo, Professor of Otorhinolaryngology for encouragement, and to Assistant Professor H. Masasmune for kind direction during the Whole course of this investigation.

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1. When glucosamine ehlorhydrate was transferred to a rabbit per os in dosis of 2gm. per kgm body weight, it is scarcely detected in blood. In urine it appears ad maximum between the 5th and the 7th hour after ingestion. The renal threshold of this compound is very low.
At the fifth hour, it is piled up the most in the mucosa of small intestine and next to it in liver, kidney, and the mucosa of stomach. The amount in pancreas was almost negligible.
The capacity of the body to tolerate it is less than 2gm. for kgm body weight.
2. When ingested subcutaneously, it appeared quickly in urine and the content of blood was highest at between the second and the third hour. At the third hour, the distribution of the substance among tissues was in the following order, decreasing from left to right.
Liver, kidney, stomach mucosa.
Besides these organs, the mucous membrane of the small intestine, pancreas and lung were found to have caught it distinctly.
Sincere thanks are due to Professor K. Kodama for criticisms and advice; to Dr. M. Mita, Professor of Pediatry, for encouragement; and to Assistant Professor H. Masamune for kind direction throughout this investigation.

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1. B. pyocyaneus has a chondrocarbohydrase as well as streptococcus haemolyticus and staphylococcus flavus have.
2. No chondrosulfatase was proved to be present in B. pyocyaneus.
3. Chondroitinsulfuric acid is a suitable source of pyocyanine.
4. The spectrophotograph of pyocyanine in hydrochloric acid reveals three absorption bands at 4700, 3800 and 2750 A
The writer is indebted to Professor K. Kodama for kind criticisms, to Dr. I. Kubo, Professor of Otorhinolaryngology, for continuous encouragement and to Assistant Professor H. Masamune for direction in the course of this investigation.

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Upon the fate of glucosamine administered to normal rabbits, the following points were noticed.
1. Gly cogenesis was observed, but to a less extent than when the amount of glucose equivalent to glucosamine was given.
2.The glucosamine is secreted partially into urine. Ammonia-and urea-nitrogen increased also in urine but the sum of the increments of ammonia-, urea- and aminosugarnitrogen did not reach the amount of the total extranitrogen. No reducing substance except glucosamine was eliminated in urine under the experimental conditions adopted.
3. Lactic acid was secreted into urine, when glucosamine was administered, but not at all when equivalent glucose was applied, though lactic acid content of blood is the same in both sugars.
4. pH of urine decreases even when glucosamine chlorhydrate was given after being neutralized.
5. Fructosazine was not detected in urine.
Sincere thanks are due to Professor K. Kodama for criticism and advice; to Dr. M. Mita, Professor of Pediatry, for encouragement, and particularly to Assistant Professor H. Masamune for kind direction and continuous encouragement throughout this work.

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1. Der PH-Wert des Kaninchenharns wird nach viele Tage langem Hunger herabgesetzt, und these Herabsetzung nimmt allmählich im Laufe der Hungertage zu.
2. Der durch Hunger herabgesetzte PH-Wert wird durch Zufuhr von Cholsäure wieder gesteigert.
3. Die Glykogenbildung der Leber, die nach vielen Hunger-tagen vermindert ist, wird durch Zufuhr von Cholsäure wieder gesteigert. Diese Steigerung geht fast parallel mit der des PH-Wertes im Harn.

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1. Atophanyl, in einer Konzentration von 1-500 mg% direkt zur Ringerlösung zugesetzt, hemmt den 0₂-Verbrauch der Leber in auffallender Weise.
2. Atophanyl, intravenös dargereicht, erhöht dagegen die Gewebsatmung der Leber auffallend; das Maximum wird schon naeh 15 Minuten erreicht. Die Milz wird dabei kaum beeinflusst.
3. Natrium salizylicum übt in einer Menge, wie sie in Atophanyl enthalten ist, keinen Einfluss auf die Gewebsatmung der Leber aus. Es ist deswegen wohl zu schliessen, dass die erhöhende Wirkung des Atophanyls dem darin enthaltenen Ato-phannatrium zuzuschreiben ist.
4. Natrium cholicum, in einer Menge über 10 mg direkt zur Ringerlösung zugefügt, hemmt die Gewebsatmung der Leber, erhöht sie aber beim Zusatz von 1 mg. Dasselbe Salz, wie auch Natrium dehydrocholicum, erhöht auffallend den 0₂-Verbrauch der Leber bei intravenöser Injektion, während die Milz kaum beeinflusst wird. Diese Erhöhung erreicht sehon 30 Minuten nach Injektion ihr Maximum.
5. Unter den Diuretika wirken Euphyllin und Salyrgan, welche eine kräftige Cholerese entfalten, erhöhend auf den 0₂-Verbrauch der Leber und zwar erreicht dessen Erhöhung 30-60 Minuten nach Injektion sein Maximum. Die Milz bleibt kaum beeinflusst. Novasurol, welches nicht choleretisch einwirkt, übt dagegen keinen Einfluss auf die Gewebsatmung sowohl der Leber als auch der Milz aus.
6. Aus diesen Tatsachen ist wohl der Sehluss zu ziehen, dass die Cholerese mit dem erhöhten 0₂-Verbrauch der Leber einhergehen wird.
7. Eine Ausnahmestellung scheint aber Avertin und Chloralhydrat einzunehmen; sie wirken zwar sehr kräftig choleretisch, aber eher herunterdrückend auf den 0₂-Verbrauch der Leber ein.
Zum Schlusse habe ich die angenehme Pflicht zu erfüllen, Herrn Prof. S Nakagawa für seine Anregung und Anleitung zu dieser Arbeit meinen herzlichsten Dank auszusprechen.

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