1. When succinate was incubated aero-bically with the resting cells of
Brevibacterium flavum No. 2247, α-ketoglutarate, or glutamate in the presence of ammonium salts, was accumulated. The addition of semicarbazide, of arsenite, and of monofluoroacetate caused the accumulation of oxaloacetate, of pyruvate, , and of citrate, respectively, instead of α-ketoglutarate. Similar results were obtained with the oxidative metabolism of L-malate, which was found to be formed from fumarate. From the inhibition experiments, following metabolic sequence of succinate was suggest-ed: succinate/fumarate/malate/oxaloacetate. pyruvate/citrate/α-ketoglutarate/glutamate.
2. When glyoxylate was incubated aero-bically with the resting cells, 2 moles of CO
2 per mole of glyoxylate was obtained, indica-ting the complex oxidation. The reaction was accelerated by the addition of acetate. The anaerobic metabolism of glyoxylate wass observed only in the presence of succinate, with the formation of citrate.
The authors are indebted to Dr. H. Oeda, Mr. S. Motozaki, and Mr. T. Tsunoda of our laboratory for the helpful advices and encourage-ment.
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