1. The cystine content of hairs and feathers of many kinds of animals was determined by the Iodine Method.
2. Some difference was observed in the cystine content of the hair, even in the albino rats of the same litter, of the same sex, which were fed with the same diet under similar condition.
3. In spite of the great difference of nutritive condition and growth of albino rats, fed with or without addition of vitamin A, the cystine content of their hair showed only a little difference when the diet was rich in protein or cystine.
4. The cystine content in the hair of animals, such as rats, cows, sheep, pigs and rabbits was, as a rule, higher in the old animals than in the young. The same fact was also observed inn the cases, of a parent and a child, and of the same animal in different periods of growth.
5. According to the results of experiments performed with animals of the same age, such as rabbits, sheep, rats, cows and oxen, in the majority of cases the cystine content of the hair was higher in females than in males.
6. The relation between the cystine content of the hair and feathers of animals and their races was studied for more than ten species of animals.
7. The cystine contents of hairs and feathers, which are located in the different parts of bodies, were compared for more than twenty species of animals.
8. It was shown, in the case of the horse's mane and tail hairs, that the cystine content of the terminal part is less than that of the other parts.
9. The cystine content varies with different parts of feathers, that of barbs is always more than that of the rhachis.
In conclusion the authers wish to express their thanks the Masters and Messieurs of the kyoto Memorial Zoological Garden and the Osaka Municipal Zoological Garden, from where the majority of the materials for the present research were obtained.

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In der grauen Substanz des Grosshirns, wo vorzugsweise eine Konzentration der Zellen stattfindet, finden wir doppelt so viel Eiweisskörper wie Lipoide vor, und in der weissen Substanz, wo wir mit einem Übermass von Leitungsbahnen, die zwecks Isolation mit Lipoiden umgeben sind, zu tun haben, konstatieren wir ein Übergewicht der Lipoide, die die Eiweisskörper um das 11/2 bis Doppelte ubertreffen.
Im Kleinhirn, wo mit unbewaffnetem Auge ein Überwiegen der graven Substanz zu ersehen ist, wo gleichermassen Leitungsbahnen verlaufen, bleiben die Lipoide hinter der Eiweissmenge zurück. Tin verlängerten Mark, wo die Leitungsbahnen massenhaft heruntersteigen, wo aber gleichfalls Kerne gehäuft auftreten, tritt ein Ausgleich zwischen Lipoiden- und Eiweissmenge ein,
Die Altersveränderungen kommen am deutlichston in den ersten 4-5 J. zur Äusserung, und finden ihren Ausdruck sowohl in einer Zunahme der Stickstoffverbindumgen wie auch vorzugsweise im Ansteigen der Lipoide. Hieraus sind wir den Schluss zu ziehen bereehtigt, class bereits im embrionalen Zustand und ganz besonders nach der Geburt, in sämtlichen Hirnabschnitten eine stärkere Zunahme der Zahl der Assotiationsfasern, und ganz besonders in der weissen Substanz des Gross- und Kleinhirns, stattfindet.

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1. The ultraviolet absorption spectra of purified ricinuslipase, urease, tyrosinase, casein, eggalbumin and a number of amino acids have been measured.
2. The absorption spectrum of ricinuslipase showed a band with a maximum at 278mμ.
3. Urease showed a band with its maximum at 265mμ at pH 7.
Fig. 6. Absorption eurve of tyrosin tryptophane, phenylalanine, (a) tryptophane. (b) tyrosin. (e) phenylalanine.
4. Tyrosinase showed a band with a maximum at 276mμ, the band shifted towards the longer wavelengths in an alkaline solution and towards the shorter wavelengths in an acid solution without alternation of absorption curve.
5. Casein showed a band with a maximum at 277mμ (pH 9.2), the band shifted towards the longer wavelengths in an alkaline and the shorter wavelengths in an acid solution without alternation of absorption curve.
6. A band with a maximum at 280mμ was found in eggalbumin.
7. Aliphatic amino acids showed no appreciable absorption band.
8. Aromatic amino acids, tyrosin tryptophane and phenyl-alanine have given absorption with a maximum at 275mμ, 280mμ and 262mμ respectively.
The author is greatly indebted to Prof. Dr. K. Iiodama for his kind criticisms and suggestions throughout this research.

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A preparation method of β-N-monoacetylglucosaminidase, the several conditioning factors therein and the stability of the enzyme were described.
Thanks are due to the Foundation for Promotion of Scientific and Industrial Research of Japan for grants which have defrayed a part of the cost of this investigation.

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1. The optimal pH for action of animal β-N-acetylgluco-saminidase lies between 3.8 and 4.1. It is not shifted by purification of the enzyme.
2. k₁K_M+k₂K_M' of the reaction is constant throughout the whole stages. The monomolecular formula does not hold.
K, K_M and K_M' at 35°C and Q were 4.398, 333, 208 and 34452. The optimal temperature is 40°C or adjacent to it.
3. The present enzyme is, in all probability, not able to attack β-glucosaminides.
The author wishes to express acknowledgement to the Foundation for Promotion of Scientific and Inustrial Research of Japan for a grant toward the expenses of this work.

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