The Kurume Medical Journal

A SIMPLE AND RAPID ASSAY FOR COLLAGENASE ACTIVITY USING FLUORESCENCE LABELED SUBSTRATE

KATSUMI SUGIYAMA, KENJI YAMAMOTO, OSAMU KAMATA, NOBUO KATSUDA

1980 Volume 27 Issue 2 Pages 63-69
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.63

JOURNAL FREE ACCESS

Show abstractHide abstract

A new assay method was developed for bacterial and animal collagenase activity, using fluorescence-labeled soluble collagen as a substrate. The enzyme reaction was carried out at 35°C in an incubation medium containing the substrate and 0.25 M glucose and the intensity of fluorescence emitted by the digested product was measured on a 50% dioxane extract of the mixture. Linear relationships were observed between fluorescence intensity and both enzyme activity and incubation time. It was confirmed that the method was a simple and rapid method for the assay of collagenase activity with high precision.

View full abstract

Download PDF (720K)
DETERMINATION OF HOMOZYGOTE VS HETEROGOTE OF BLOOD GROUP ANTIGENS WITH PROTEIN A

ALAN LOREN, DAVID CHARMAN, DEAN JABS, M. MITSUO YOKOYAMA

1980 Volume 27 Issue 2 Pages 71-77
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.71

JOURNAL FREE ACCESS

Show abstractHide abstract

By sensitizing human red blood cells with corresponding antisera specific to blood group antigens and subsequent rosette formation with Staphylococcus aureus, we were able to distinguish homozygosity of the antigens from heterozygosity. Three blood group systems : Rh, Kell and Kidd were used in this study. In the Rh blood group system, we were able to demonstrate the dosage effect of the Rh₀ (D) antigen by rosette assay in addition to determining that the deletion of the C and/or E alleles increased the rosette forming cell count when red blood cells with different genotypes were sensitized with anti-D. Our assay proved to be simple, specific, highly reproducible, and can be adapted to study virtually any antigenic markers on a cell surface.

View full abstract

Download PDF (2956K)
DEMONSTRATION OF DOSE RESPONSE WITH IMMUNOBEADS IN ROSETTE FORMING CELL COUNTS USING HOMOZYGOTE AND HETEROZYGOTE OF THE Rh₀ (D) ANTIGEN

YOSHINOBU MATSUO, ALAN LOREN, M. MITSUO YOKOYAMA

1980 Volume 27 Issue 2 Pages 79-83
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.79

JOURNAL FREE ACCESS

Show abstractHide abstract

An Immunobead rosette assay was used to differentiate human RBC that were homozygous for the Rh₀ (D) antigen from human RBC that were heterozygous for the Rh₀ (D) antigen. Additionally, we confirmed the dose response phenomena observed in rosette assays when the percentage of rosette forming cells was evaluated as a function of antibody titer used for sensitization. The Immunobead rosette assay provided a simple, yet effective method for differentiating the genotypes of cell surface antigens in the Rh system and could feasibly be adapted to study virtually any cell surface antigens.

View full abstract

Download PDF (2488K)
DEMONSTRATION OF THE DOSAGE EFFECT OF Rh₀ (D) ANTIGENS ON HUMAN RED BLOOD CELLS BY AN EA ROSETTE ASSAY

MASAKAZU HORITA, ARTHUR D. JABS, BARBARA A. RICKER, ALAN LOREN, M. MIT ...

1980 Volume 27 Issue 2 Pages 85-92
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.85

JOURNAL FREE ACCESS

Show abstractHide abstract

Utilizing an EA rosette assay, we were able to distinguish genotypic differences of the Rh₀ (D) antigen on human red blood cells (RBC) based on the number of rosettes formed between human peripheral B lymphocytes and RBC optimally sensitized with anti-Rh₀ (D) antibody. In this study, RBC with possible Rh genotypes R₁R₁ (CDe/CDe), R₁r (CDe/cde), R₂R₂ (cDE/cDE), R₂r (cDE/cde) and R₁R₂ (cDe/cDE) were sensitized with either Rh₀GAM (anti-D) or Ripley serum (anti-G). When RBC were optimally sensitized with Rh₀GAM, R₁r formed 60% as many rosettes as R₁R₁ and R₂r formed 64% as many rosettes as did R₂R₂. Using Ripley serum for sensitization and comparing the number of rosettes formed, R₁r exhibited 70% of R₁R₁ and R₂r showed 76% R₂R₂. Furthermore, R₁R₁ cells sensitized with Ripley serum formed a higher percentage of rosettes than R₁R₂ or R₂R₂.

View full abstract

Download PDF (2890K)
HEMATOCELE OF THE SPERMATIC CORD

NOBUYUKI MIYOSHI, TAKAHIRO TOKITO, KOUSAKU ETO, MASAHIRO ARAKAWA

1980 Volume 27 Issue 2 Pages 93-95
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.93

JOURNAL FREE ACCESS

Download PDF (2192K)
ADENOMATOID TUMOR OF THE SPERMATIC CORD

NOBUYUKI MIYOSHI, SHINSHI NODA, KOUSAKU ETO, MASAHIRO ARAKAWA

1980 Volume 27 Issue 2 Pages 97-100
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.97

JOURNAL FREE ACCESS

Show abstractHide abstract

We have encountered a case of tumor of the spermatic cord, a relatively rare disease. The case is a 60-year old man. Histologic examinations revealed that the tumor was an adenomatoid leiomyoma. The case of adenomatoid tumor hitherto reported in Japan totals 66 inclusive of ours. However, they are reported as primary epididymal tumor in literature. Ours is also a tumor which developed in the epididymal region, but it is most likely to have originated from the tunica vaginalis of the spermatic cord judging from the macroscopic and histologic findings.

View full abstract

Download PDF (2357K)
PRIMARY LEIOMYOSARCOMA OF THE OMENTUM

AKIRA TANIMURA, TAKANORI CHO, MASATOSHI NOHARA, MASANORI YAMASHITA, YO ...

1980 Volume 27 Issue 2 Pages 101-105
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.101

JOURNAL FREE ACCESS

Show abstractHide abstract

Two cases of leiomyosarcoma developed from great omentum and gastrohepatic omentum respectively are reported. The Case 1 is a 52-year-old female with chief complaint of a mass in the abdomen, which was found to be a primary gastrohepatic tumor upon laparotomy, and histologically diagnosed as leiomyosarcoma. The Case 2 is a 46-year-old male with chief complaint of precordial pain. Upon laparotomy a part of tumor was found adhered to gastric serosa. The tumor was excised together with stomach. Microscopic observation revealed infiltration spread in the gastric wall. In either case no metastatic focus was found at the time of operation, and no recurrence is expected although 3 years and 7 years, respectively, passed after the operation.

View full abstract

Download PDF (4583K)
NON-FUNCTIONING ISLET CELL CARCINOMA DIFFICULT TO DIFFERENTIATE FROM DUODENAL CARCINOMA

A CASE REPORT

HIRONARU FUKUSHIMA, AKIRA ADACHI, SADAAKI KUWAO, KOHTA YATSUKA, HIROSH ...

1980 Volume 27 Issue 2 Pages 107-112
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.107

JOURNAL FREE ACCESS

Show abstractHide abstract

A 52-year-old male who had non-functioning islet cell carcinoma with liver metastasis that was clinically misdiagnosed as a duodenal carcinoma was presented. The tumor, 6 × 5 × 4 cm, originated from the pancreatic head and infiltrated to the pyloric region and the superior portion of the duodenum. In addition to this case report, we have discussed about 11 case-reports collected in Japan.

View full abstract

Download PDF (5872K)
AORTITIS SYNDROME AND HYPERTHYROIDISM

A CASE REPORT AND REVIEW OF THE JAPANESE LITERATURE

YOSHIHIRO SATO, MASARO KAJI, SHUNJIRO KAGIYAMA, KENICHI NAGASAWA

1980 Volume 27 Issue 2 Pages 113-117
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.113

JOURNAL FREE ACCESS

Show abstractHide abstract

A patient with aortitis syndrome and hyperthyroidism was reported. A review of the literature revealed 14 other cases with aortitis syndrome and autoimmune thyroid diseases in Japan. These cases may add to other indirect evidence that aortitis syndrome may be caused by an autoimmune mechanism.

View full abstract

Download PDF (2928K)
CORRELATION OF CORONARY ARTERIOGRAM, ELECTROCARDIOGRAM AND EXERCISE TEST IN SPONTANEOUS ANGINA

A POSSIBLE CAUSE OF ANGINAL ATTACK

YASUO OHKITA

1980 Volume 27 Issue 2 Pages 119-131
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.119

JOURNAL FREE ACCESS

Show abstractHide abstract

Combination of coronary artery spasm and organic coronary stenosis has been postulated as a possible cause of spontaneous angina. The present study was performed to clarify the role of organic stenosis and spasm of coronary artery in spontaneous angina according to the coronary arteriography and the shifts of ST-segment in the 12-lead ECG during anginal attack in 53 patients with spontaneous angina. In 33 cases of ST elevation during attack, only 14 cases (42 %) had organic stenosis. Three cases had multivessel disease (MVD), and 3 cases had collateral vessels. The incidence of organic stenosis in the corresponding artery to the electrocardiogram was higher in the anteroseptal area than in the inferior area. In 20 cases of ST depression during attack, 19 cases (95 %) had organic stenosis. Fourteen cases had MVD and 10 cases had collateral vessels. The incidence of organic stenosis, including MVD, and collateral vessels was higher in cases of ST depression than in cases of ST elevation. And also the incidence of positive response to the exercise test is higher in cases of ST depression than in cases of ST elevation. The results suggest that coronary spasm is the main cause of spontaneous angina in cases of ST elevation, and organic stenosis of coronary artery in addition to coronary spasm is the main cause of the anginal attack in cases of ST depression.

View full abstract

Download PDF (5859K)
FEATURES OF CT CISTERNOGRAM IN ARACHNOID CYST OF QUADRIGEMINAL CISTERN

TAKASHI HAYASHI, AKIHIKO KURATOMI, SHINKEN KURAMOTO

1980 Volume 27 Issue 2 Pages 133-137
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.133

JOURNAL FREE ACCESS

Show abstractHide abstract

The authors report a case of an infant girl referred to our clinic because of an enlarged head and a delay in taking her first steps. Hydrocephalus was diagnosed, a ventriculo-peritoneal shunt was performed immediately, and a Metrizamide CT citernogram was run. The features of Metrizamide CT cisternogram of the arachnoid cyst situated in the tentorium at the posterior of the third ventricle are described and the mechanism of fluid accumulation in the cyst.

View full abstract

Download PDF (4834K)
CT FINDINGS IN AN ALOBAR HOLOPROSENCEPHALY ASSOCIATED WITH DANDY-WALKER'S CYST

TAKASHI HAYASHI, SHINKEN KURAMOTO, SHIGEYUKI TAKAGI, NOBUTO KOJYO, KEN ...

1980 Volume 27 Issue 2 Pages 139-142
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.139

JOURNAL FREE ACCESS

Show abstractHide abstract

The authors describe a case of alobar holoprosencephaly with a huge cyst in the posterior fossa, possibly representing a Dandy-Walker's cyst, and marked cerebellar hypoplasia in the absence of midline facial dysmorphias that has recently been encountered at our clinic.

View full abstract

Download PDF (2113K)
THE EFFECT OF 4-AMINOPYRIDINE ON THE NICOTINIC TRANSMISSION OF BULLFROG SYMPATHETIC GANGLION CELLS

TAKASHI AKASU, MICHIHISA YAMADA

1980 Volume 27 Issue 2 Pages 143-148
Published: August 25, 1980
Released on J-STAGE: August 11, 2009

DOIhttps://doi.org/10.2739/kurumemedj.27.143

JOURNAL FREE ACCESS

Download PDF (732K)

Register with J-STAGE for free!