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Volume 27 (1986)
- Issue 6 Pages 615-
- Issue 5 Pages 451-
- Issue 4 Pages 343-
- Issue 3 Pages 203-
- Issue 2 Pages 145-
- Issue 1 Pages 1-
Volume 27, Issue 2
Displaying 1-8 of 8 articles from this issue
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Tamio MAITANI1986 Volume 27 Issue 2 Pages 145-155
Published: April 05, 1986
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (1599K) -
Ken-ichiro MINEGISHI, Hideo KUREBAYASHI, Michio SATO, Tomoko NISHIMAKI ...1986 Volume 27 Issue 2 Pages 156-162_1
Published: April 05, 1986
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSGroups of 10-15 male Sprague-Dawley rats were fed diets containing erythrosine (FD & C Red No. 3) at levels of 0 (control), 0.25, 1.0, or 4.0% for 2 weeks. An increase in thyroxine (T4) and a decrease in triiodothyronine (T3) levels, and an inhibition of thyroid peroxidase activity (TPO) were observed at higher dosage levels. However, there were no statistically significant differences between the treated and control groups in the values for thyroid stimulating hormone (TSH). The observed effects appeared to depend to some extent on the particle size of the administered erythrosine.View full abstractDownload PDF (718K) -
Metabolism of Propyl Gallate by the Intestinal Bacteria in the RatToshio NIIMURA, Chizuko OTANI, Tsutomu YAMAHA1986 Volume 27 Issue 2 Pages 163-170_1
Published: April 05, 1986
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSThe metabolism of propyl gallate (PG) by the intestinal bacteria of rats was studied. In the anaerobic incubation of PG with fecal microorganisms, the formation of resorcinol (R) from PG was observed. One strain of Klebsiella pneumoniae, a PG-hydrolyzing bacterium, was isolated from the feces of rats. The strain hydrolyzed PG to gallated (G), and further decarboxylated G to pyrogallol (P). Seven strains of Streptococcus faecalis and two strains of Enterobacter aerogenes as G-decarboxylating bacteria were isolated from the feces of rats. All strains accumulated P in the medium from G. P-dehydroxylating bacteria could not be isolated as a pure culture. However, active mixed bacteria consisting of Escherichia coli and Streptococcus sp. were obtained by selective culture in medium containing P. The mixed bacteria showed an inducible P-dehydroxylating enzyme activity producing R from P. This activity was maintained in the dried cells.
These observations support the participation of intestinal bacteria in the metabolism of PG.View full abstractDownload PDF (981K) -
Masakazu TSUTSUMI, Hiroyuki YAMADA, Fumio OHOTAKA, Jea Kyum LEE, Tadao ...1986 Volume 27 Issue 2 Pages 171-175_1
Published: April 05, 1986
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSBacteria such as Escherichia coli IFO 3301, Proteus vulgaris IFO 3045, Bacillus subtilis IFO 3037 and Staphylococcus aureus IU-23 were able to grow in swine blood, causing putrefaction of the blood. The putrefaction of the blood could be easily recognized by means of a sensory test (putrefactive odor) before any change in pH took place. The growth of Staphylococcus aureus caused gelatinization of the blood.
Though Proteus vulgaris grew in a nutrient broth containing 1% glycine and 3% sodium chloride, Escherichia coli, Pseudomonas fluorescens IFO 3081, Salmonella typhimurium, Aeromonas hydrophila, Bacillus subtilis and Staphylococcus aureus could not grow in the same medium. On the other hand, no growth of Proteus vulgaris was observed in swine blood containing 1% glycine and 3% sodium chloride under the same conditions. In practice, blood containing these substances did not putrefy within 24 hours at 30°C.
Hydrolysis of swine blood proteins by commercial proteases, Bioprase SP-4, Denazyme AP, Papain and Actinase AS, was carried out in the presence of 1% glycine and 3% sodium chloride. The blood proteins were readily broken down into simpler compounds by these enzymes.View full abstractDownload PDF (601K) -
Michiko ITO, Mika SATO, Nobuyuki SHIBATA, Shigeo SUZUKI1986 Volume 27 Issue 2 Pages 176-179_1
Published: April 05, 1986
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method was developed for the quantitative determination of iron in foods containing large amounts of sodium chloride by adopting a combination of coprecipitation with zirconium hydroxide and flame atomic absorption spectrometry.
A linear calibration curve was obtained based on the recovered amount of iron added to 10% aqueous solution of sodium chloride in the range from 1 to 5ppm.
The results of recovery tests of iron added to the incinerated products of soy sauce and bean paste indicated that the method gave satisfactory results with a recovery of iron of 98 to 100%.View full abstractDownload PDF (428K) -
Yoshio UENO, Toshitsugu TANAKA, Akihiko HASEGAWA, Zhou-Han HU, Da-Dao ...1986 Volume 27 Issue 2 Pages 180-182_1
Published: April 05, 1986
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSScabby wheat that was naturally infected with head blight was collected in 1976 from rural districts of Shanghai. GC-ECD, GC/MS and HPLC analyses of the ethanol-acetone extract revealed the presence of deoxynivalenol, nivalenol, and zearalenone. This is the first report to demonstrate chemically the natural occurrence of trichothecenes and zearalenone in scabby wheat in China.View full abstractDownload PDF (328K) -
Kimihito OTSUKA, Akira SUGITANI, Fujizo YAMADA1986 Volume 27 Issue 2 Pages 183-186_1
Published: April 05, 1986
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA method is described for the quantitative determination of nifurpirinol (NPN) in fish by thin layer chromatography (TLC) and fluorodensitometry. The sample was mixed with anhydrous sodium sulfate and extracted with dichloromethane. The extract was cleaned up by Florisil column chromatography. The acetone eluate was spotted on the TLC plate and developed with a solvent system of chloroform-ethyl acetate (20:80). After drying, the TLC plate was kept in pyridine vapor for 20 minutes, and then irradiated for 6 minutes with a long-wave UV lamp. The fluorescence intensities of spots were quantitatively measured by using a fluorodensitometer with excitation at 420nm and emission at 550nm.
The recoveries of NPN added to fish were in the range from 82.1% to 88.7% with an average of 84.9% at levels from 0.05 to 0.1ppm. The minimum detectable amount of NPN was 1ng. The calibration curve was linear over the range from 2.5ng to 30ng.View full abstractDownload PDF (410K) -
Rapid Determination of Diethylene Glycol in Wine by Gas Chromatography using Alumina Column Clean-upYoshihiro HORI, Takao CHONAN, Makoto NISHIZAWA1986 Volume 27 Issue 2 Pages 187-189_1
Published: April 05, 1986
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDiethylene glycol (DEG) in wine was purified by alumina column chromatography and analyzed by gas chromatography (GC) with a flame ionization detector. Wine was easily cleaned up by the use of an alumina column with methanol as an eluent. Therefore, the determination of DEG by GC could be completed in less than 10 minutes. The reroveries of DEG added to wine at levels of 40 and 200ppm were 86.2 and 89.3%, respectively, and the detection limit was 10ppm. This method is rapid and simple, and is convenient for large numbers of analyses as compared with the Extrelut column method.View full abstractDownload PDF (247K)
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