To validate an LC-MS/MS method using a strong anion exchange cartridge for simultaneous determination of fumonisin B
1, B
2 and B
3 in corn, an inter-laboratory study was performed in 9 laboratories using one fumonisin-negative corn sample, three spiked corn samples (FB
1: 100–1,000 μg/kg, FB
2 and FB
3: 10–100 μg/kg) and two naturally contaminated corn samples. The recoveries were in the ranges of 79.7–87.2% for FB
1, 78.6–103.2% for FB
2 and 80.1–92.8% for FB
3. The relative standard deviations for repeatability (RSDr) ranged from 3.7 to 8.0% for FB
1, from 2.6 to 15.3% for FB
2 and from 4.3 to 9.7% for FB
3. The relative standard deviations for reproducibility (RSDR) for FB
1, FB
2 and FB
3 were in the ranges of 6.3–10.1%, 5.9–18.7% and 9.3–16.0%, respectively. The HorRat values for all analytes ranged from 0.2 to 0.9. The difference of the trueness between the two kinds of commercially available anion exchange cartridges used in this study was not significant (
p>0.05). Surveillance for fumonisins in corn grits was performed using the validated method. All of the samples were contaminated with fumonisins and the mean concentrations for FB
1, FB
2 and FB
3 were 118.1, 37.3 and 17.9 μg/kg, respectively. These results indicated that the method for simultaneous determination of FB
1, FB
2 and FB
3 in corn was successfully developed and validated.
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