1. The investigation was made to study the differential nature of the chromosomes and other cell components on the basis of their phosphatase activity.
2. To test alkaline phosphatase activity, Gomori's revised method and for acid phosphatase activity, Glick and Fisher's adaptation of Gomori's method were applied.
3. Differential precipitation has been very high in all the cellular components in case of alkaline phosphatase, while with acid phosphatase the activity has been very low and uniform.
4. In polyploid cells the nucleoli are practically indistinguishable, other cellular components giving the same reaction at a much reduced rate.
5. The high alkaline phosphatase activity is due to the presence of this enzyme in both nucleic acid and protein components of the chromosomes.
6. End portions of chromosomes contain a higher dosage of the enzyme. In the constriction regions it is insignificant, but the satellite thread is positive in some of the plates. This implies that satellite stalks and secondary constriction segments which are functionally similar, may differ in the quantity of phosphatase positive substances or nucleic acid.
7. Decreased phosphatase activity in polyploid cells is due to the greater dispersion of the enzyme as a result of increase in cell surface.
8. Loss of distinction of nucleoli in polyploid cells is the result of diminution of viscosity difference between the nucleoli and nucleoplasm caused by chemical treatment.
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