CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
Volume 4 , Issue 2
Showing 1-6 articles out of 6 articles from the selected issue
  • W. A. Becker
    1933 Volume 4 Issue 2 Pages 135-157
    Published: March 31, 1933
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    In der vorliegenden Arbeit wird über abnorme Zellteilungen berichtet, welche nach Vitalfärbung mit Methylenblau und Neutrairot entstehen.
    1. Methylenblau ruft starke Störungen vom Typus “Entqueliungsformen” in der Kernteilung hervor; durch Neutralrot werden leichte Abnormitäten desselben Typus nur durch stark konzentrierten Lösungen hervorgerufen.
    2. Die Brücken- und Strangbildungen, welche die auseinander-weichenden Chromosomen verbinden und sowohl in vivo wie auf fixiertem Material Spindelfasern vortäuschen können, sind als Ausdruck der schwachen “Entquellung” der Kernstrukturen aufzufassen.
    3. Die Rekonstruktionsstörungen in der Telophase, welche nach Färbung mit Methylenblau auftreten, können unter Umständen mittels Narcose behoben werden.
    4. Zellen, welche in dem normalen Fortgang der Karyokinese durch Methylenblau aufgehalten werden, vermögen in jeder Kernteilungsphase (Prophase, Metaphase) einen rudimentären Phragmoplasten, eine Zellplatte und eine Zellmembran ausbilden.
    5. Die stark “entquellende” Wirkung von Methylenblau auf den Karyokineseverlauf, im Gegensatz zur schwachen Wirkung von Neutralrot, steht wahrscheinlich mit der leichten Reduktionsfähigkeit dieses ersten Vacuolarfarbstoffes im Zusammenhange.
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  • Petr F. Milovidov
    1933 Volume 4 Issue 2 Pages 158-173
    Published: March 31, 1933
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    1. The tatest attempts to connect the chondriosomes with nuclear matter must be considered just as inconsistent as the previous ones.
    2. The chondriosomes of the vegetative cells give a negative nueleal reaction and therefore do not contain any thymonucleic acid which is the most characteristic element of the cell nucleus.
    Yet the absence of nucleal reaction of the chondriosomes does not depend an their destruction during fixation or the hydrolysis, as a simple restaining of the preparations with iron-hematoxylin or acidfuchsin can prove their presence.
    3. Chondriosomes do not contain any “chromatin” and are not in any genetic connection with the Gell nucleus.
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  • I. Chromosomes in the spermatocyte divisions of five North American species of toads
    Emil Witschi
    1933 Volume 4 Issue 2 Pages 174-181
    Published: March 31, 1933
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    The chromosomic constitution of five American species of toads is much the saure as that described by previous authors for European and Japanese species of the same genus. The haploid set consists of 6 large and 5 small chromosomes. The fifth chromosome shows peculiarities that bear out its homology with the supposed sex chromosome of Rana temporaria. The importance of comparative studies in amphibian chromosomes with respect to the problem of the evolution of the sex chromosome mechanism is emphasized.
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  • Syôzô Adati
    1933 Volume 4 Issue 2 Pages 182-188
    Published: March 31, 1933
    Released: March 19, 2009
    JOURNAL FREE ACCESS
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  • Namio Shinke, Michio Shigenaga
    1933 Volume 4 Issue 2 Pages 189-221
    Published: March 31, 1933
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Histochemical tests were made wich the resting as well as dividing nuclei in plants with FEULGEN's nucleai-reaction method for the test of thymusnucleic acid, MILLON'S, the xanthoproteic and the biuret reaction method for Proteins, and CHRISTELLER'S, CIACCIO's and other methods of testing for lipoids. The solution analysis method and staining according to PAPPENHEIM-UNNA'S method were also used as supplemen-tary means of testing the results obtained with the methods named above. The results we obtained with these methods are as follows:
    1) The nuclear reticulum and chromosomes show reactions of thymusnucleic acid, lipoids and proteins.
    2) In the chromosomes the thymusnucleic acid reaction is found exclusively in the spiral portion.
    3) The chromosomes are far more easily dissolved by lipoid solvents and nucleoprotein solvents than the resting nucleus is (Tables 1 and 2).
    4) Of the two morphological components f of chromosomes, the spiral portion and matrix, the former is more resistant to the action of lipoid solvents than the latter.
    5) There is some evidente which suggests that certain material changes may take place in the nuclear components during mitosis.
    6) The nucleus of Spirogyra sp. shows no thymusnucleic acid reaction but a protein reaction, forming a striking contrast to the other plants investigated.
    7) The karyolymph show no positive result in any of the tests for thymusnucleic acid, Lipoids, and proteins, but the result obtained with the precipitation method suggests that there may be lipoid in the karyolymph.
    8) The nucleolus is shown to contain lipoids, but no thymusnucleic acid.
    9) The spindle fibers and the phragmoplast are shown to consist mainly of proteins and Lipoids in a mixed or a combined form.
    In concluding, the authors wish to express their sintere thanks to Prof. Y. KUWADA for his kind guidance throughout the investigation.
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  • Bungo Wada
    1933 Volume 4 Issue 2 Pages 222-227_1
    Published: March 31, 1933
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    1. Durch das Auseinanderziehen einzelner Chromosomen der Pollenmutterzelle von Tradescantia reflexa mit den Mikronadeln lösen sich die Spiralen der Chromonemata kontinuierlich auf und dehnen sich zu langen feinen Fäden aus.
    2. Bei den angequollenen Pollenmutterzellen wird das Vorhandensein einzelner Chromosomen durch das Auseinanderziehen des optisch homogen aussehenden Chromosomenteils mit den Nadeln nachgewiesen.
    3. Die Spiralstruktur der somatischen Chromosomen tritt erst durch das Auseinanderziehen derselben mit den Nadeln im Medium in Erscheinung.
    Zum Schluß sei es mir gestattet, meinem hochverehrten Lehrer, Herrn Prof. eurer. Dr. K. FUJII, der mich mit stetiger Anregung unterstützte, meinen verbindlichsten Dank auszusprechen.
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