CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
Volume 31 , Issue 4
Showing 1-12 articles out of 12 articles from the selected issue
  • H. Hausen, A. Lange, J. Fröhlich
    1966 Volume 31 Issue 4 Pages 339-348
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Der 1948 klonierte L-Stamm NCTC 929-bei uns über 155 Passagen in TCM 199 mit 5% Kälberserumzusatz gehalten wurde im Hinblick auf genetische Veränderungen nach erhöhtem Serumzusatz überprüft.
    Nach Zusatz von 10 und 20% Kälberserum fand sich bereits nach 10 Passagen eine signifikante Änderung des Chromosomenspektrums im Sinne einer Einengung. Bei 40% Serumzusatz ließ sich nach 60 Passagen eine solche Änderung nachweisen, die gleichzeitig bei Chromosomenanalyse in 70% einen Genotyp aufzeigte, der sich nicht im Ausgangsstamm finden ließ.
    Zusätzliche Untersuchungen eines als Zellverunreinigung aufgetretenen L-Stammes (LZ) sowie eines klonierten L-Stammes (La 10) zeigen auch hier signifikante Abweichungen vom Ausgangsstamm auf.
    Der Ausgangsstamm liegt mit seinen polyploiden Mitosen deutlich über allen weiteren untersuchten Stämmen.
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  • Sajiro Makino
    1966 Volume 31 Issue 4 Pages 349-374
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
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  • Braj Nandan Prasad, M. B. E. Godward
    1966 Volume 31 Issue 4 Pages 375-391
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
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  • In Auseinandersetzung mit D. E. LEA
    Hideo KOMURO
    1966 Volume 31 Issue 4 Pages 392-401
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Diese Abhandlung behandelt wichtige zytologische Unterschiede zwischen kontinuierlich-fortgesetzter und der unterbrochener RÖNTGENbestrahlung auf Vicia faba-Wurzelspitzellen.
    Material: Vicia faba-Keimling und die der aus beatrahlten Samen hervorgegangenen Materialien, welche im trockenen (in Luft) und gequollenen (im Wasser) Zustand der Samen mit der Molybdän_??_ und Wolframantikathode bestrahlt worden und im Sand ausgelegt. Die erste wurde wie KML [Keimling] in Tab. 1 behandelt.
    Die Wurzelspitzen dieser beiden Keimlingen habe ich zytologisch oder die Keimlinge zuerst physiologisch, später iher Spitze zytologisch beobochtet.
    Physiologische und zytologische Untershiede zwischen beider Bestrah_??_ lungsmethoden sowie den Arten der Antikathodenmetallen waren sehr groß-Schädigungen bei unterbrochener Bestrahlung mit Molybdänantikathode waren sehr stark und RÖNTGENgeschwülste in meinem Sinne entstand früher als bei kontinuierlich-fortgesetzter Bestrahlung mit Wolframantikathode.
    Die Tatsache, die ich durch die lange, genaue Beobachtung seit 1915 angemacht habe, kann ich nicht mit der Ansicht von verstorbenem D. E. LEA übereinstimmen, wonach vor allem keine Unterschiede zwischen kontinuierlich-fortgesetzter und unterbrochener Bestrahlung bemerkt und verzögerte Chromosomen, Chromosomenbrücke, multipoläre mitotische Teilungsbilder usw. als charakteristische Kernveränderungen bei der Bestrahlung behauptet wurden. Diese Abnormitäten des Kernes entstehen immer bei Kohlenteer-behandlung auf Pflanzen und Tiere. Man bemerkt these Unterschiede auch in den Zellen von pflanzlichen und tierischen Geschwülsten-primär und experimental. Ich habe viele zytologische Beispiele von mit Kohlenteerlösung behandeltem pflanzlichem Material und von Karzinom_??_ und Sarkomgeschwülsten als ehn Zytolog in der Onkologie.
    Zytoplasmatische Veränderung in den bestrahlten Zellen spielt eine große Rolle, um die Schädigung beim bestrahlten Körper aus zulösen.
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  • Ranajit Mallick, Arun Kumar Sharma
    1966 Volume 31 Issue 4 Pages 402-410
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    The present work deals with the cytology and karyotype analysis of the seven species of the order Pandanales belonging to the three genera-Pandanus, Typha and Sparganium under three distinct families. The karyotypic studies show 2n=60 chromosomes in case of Pandanus and 2n=30 chromosomes in case of Typha and Sparganium. Meiotic studies in only one species of Sparganium show regular 15 bivalents.
    The uniformity in chromosome number with extremely small size is the characteristic features of the three genera. In Sparganium and Typha the chromosome number is 2n=30, whereas in Pandanus it is 2n=60. The chromosome morphology of the different species of the genera shows slight differences in their structure particularly in the number of satellited chromosomes.
    Cytological studies show the close affinities of the three genera thus supporting Engler and Prantl's contention. However they also indicate their advanced status, thus supporting Hutchinson's view.
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  • Dorothea Bennett
    1966 Volume 31 Issue 4 Pages 411-415
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Examination of air dried preparations of metaphase figures in mouse embryonic liver cells in which three pairs of chromosomes with satellites can be demonstrated shows clearly ring-like and end to end associations of chromosomes which appear to be spatially non-random. The chromosomes with satellites participate in these associations much more frequently than expected by chance. The possible significance of these associations in terms of nucleolus organization is discussed.
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  • Shuetu Suzuki
    1966 Volume 31 Issue 4 Pages 416-421
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    A total of 207 rabbit ova were inseminated in vitro in tubal fluid under mineral oil using capacitated spermatozoa recovered from the uterus 12 hours after mating. About 20 hours after the transfer for cultivation, 123 (59.4%) of them were at the 2- to 4-cell stages. Sixty-five of the fertilized ova were cultivated for two or three days after insemination in vitro, and 21.5% of them could be reached at over the 16-cell stage.
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  • R. S. Sadasivaiah, M. L. Magoon
    1966 Volume 31 Issue 4 Pages 422-437
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Important morphological features of 14 species and 13 hybrids were compared and it was observed that with respect to several metrical characters the hybrids were usually intermediate in some cases but in certain other cases, hybrid vigour was also met with. The dominant-recessive relationships of several qualitative characters in these hybrids have also been studied. Pachytene pairing was complete and apparently normal, followed by regular meiosis at later stages resulting in high pollen fertility and good seed setting in all the parental species except the male sterile ‘Kafir’. The interspecific hybrids studied are classified with suitable explanations into four categories based on pachytene pairing and pollen sterility. 1) Normal pairing accompanied by high pollen fertility, 2) Normal pairing accompanied by partial pollen sterility, 3) Irregularities in the pachytene pairing accompanied by partial pollen sterility, 4) Irregularities in the pachytene pairing accompanied by normal pollen fertility. Based on cytomorphological data, the interrelationships amongst the species are discussed.
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  • Laurence Levine
    1966 Volume 31 Issue 4 Pages 438-451
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    1. A relatively simple and reproducible technique is described for the time lapse cinemicrographic recording of the biographies of male germ cells in Acheta domesticus, utilising testicular fluids as a maintenance medium.
    2. Several types of biographies were recorded, depending upon whether or not cytokinesis had occurred. Although all the spermatogonia observed underwent cytokinesis, spermatocytes varied in their ability to do so. More primary spermatocytes displayed cytokinesis than secondary spermatocytes. When cytoplasmic division failed to occur, reformed nuclei either remained discrete or fused. Fusion of secondary spermatocytes was also observed. This consistently occurred in the prometaphase and fused cells always exhibited cytokinesis.
    3. The behavior of highly refractile small granular aggregates was also described. These were very mobile yet occupied predictable positions during prophase and metaphase, and had consistent configurations in late anaphase. The masses were frequently distributed assymetrically across the cytokinetic furrow.
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  • C. N. Sun
    1966 Volume 31 Issue 4 Pages 452-456
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Abutilon striatum possessing white-margin leaves was studied with light and electron microscopes. The white margin tissue was found less thick than the normal green part and lack palisade mesophyll layer under the light microscope. Electron microscopic studies indicated great alterations occurred within the plastids, at the white margin portion of the leaf blade. Three major alterations were disclosed: 1) the absence of grana, 2) the abnormality of lamellar system, 3) the occurrence of vacuole-like circular bodies. The causes of these were proposed due to a destruction of the lamellar system by vacuolization follows the termination of the differentiation of grana.
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  • A. Kabarity
    1966 Volume 31 Issue 4 Pages 457-460
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    In the present investigation, the treatment of pollen mother cells of Triticum aestivum with acetone provided an excellent material for multipolarity. It was possible to follow the formation of the spindle fibre apparatus in the case of multipolar anaphases. Stickiness and chromosome bridges were observed after treatment with high concentrations of acetone. Non-congression phenomenon and free chromosomes in anaphases were found beyond the multipolar anaphases in lower concentrations. The formation of multiple spindle apparatus was discussed and it was concluded that application of acetone may disturb the extrachromosomal mechanism leaving the intrachromosomal process to proceed normally. This disturbance is restricted to the abnormal diffusion of the spindle organisers in many directions instead of two poles.
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  • K. Ueda
    1966 Volume 31 Issue 4 Pages 461-472
    Published: December 25, 1966
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    The fine structure of a green alga Chlorogonium elongatum was studied.
    All membranes such as the plasma membrane, mitochondrial membrane, Golgi membrane, the membrane of the endoplasmic reticulum, the vacuole membrane, the chloroplast envelope and the nuclear envelope appeared as three layers.
    The thickness of these membranes was measured. The plasma membrane and the vacuole membrane were thicker than other membranes.
    The origin and the development of the vacuole were concluded to be as follows: First, small vesicles were budded off from the endoplasmic reticulum and taken into the Golgi cisternae. From the opposite end of the Golgi cisternae another kind of vesicle was pinched off. Some of these vesicles grew into provacuoles and others were taken into provacuoles. Some provacuoles may be directly derived from Golgi cisternae. Provacuoles increased in size and matured into vacuole.
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