CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
Volume 58 , Issue 1
Showing 1-16 articles out of 16 articles from the selected issue
  • V. R. K. Reddy, Renu Edwin, C.P. Suganthi
    1993 Volume 58 Issue 1 Pages 1-8
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
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  • G. K. Manna, G. C. Sadhukhan
    1993 Volume 58 Issue 1 Pages 9-19
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    The mutagenic potential of log culture (20×107 cells per ml) of the non-nodulous nitrogenfixing free living bacterium, Xanthobacter flavus in treated male parent mice after mating with different sets of virgin untreated normal females for 7 consecutive weeks and in their F1 progeny verified by lethal test in vivisected mothers and in living ones by various cytogenetic assays was found positive in each test as compared to parallel controls. In male parents as well as in both sexes of F1 embryos and adults, as the cases might be, the frequencies of chromosome aberration and micronucleated erythrocytes in somatic cells, male meiotic chromosome aberration and sperm head abnormality were strikingly high in treated series than in respective controls, indicating the same trends of cytogenetical effects in parent and F1 progeny of treated series. The results have been explained with the hypothesis that the treatment of log culture of X. flavus to male parent mice induced mutation to some genetic locus/loci which in normal state maintained the structural integrity of chromosome, but when mutated lost the control. This might have led to various cytogenetic effects in F1 progeny, in some cases caused lethality while in others (living ones) produced visible cytogenetic anomalies of various forms assessed by different testing protocols.
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  • T. A. Soman, K. V. Bhavanandan
    1993 Volume 58 Issue 1 Pages 21-26
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Spontaneous occurrence of cytomixis in PMCs of H. elastica was noticed in high atmospheric temperature. The chromatin undergoes a temporary process of agglutination before migration. Cytomixis is found to have a direct correlation with other meiotic irregularities and sterility. The origin and evolutionary significance of the process is discussed.
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  • R. R. Nair, B. Sasikumar, P. N. Ravindran
    1993 Volume 58 Issue 1 Pages 27-31
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Polyploidy in a cultivar (Collection No: 1344) of black pepper (Piper nigrum L.) and numerical chromosomal variation in its twenty progenies were identified by cytological analysis. The plant was a triploid with chromosome number 2n=78, and the progenies showed a range of variation from 2n=52 to 2n=104. Morphological types were observed with variation in chromosome number. Possible origin of the mother plant is also discussed.
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  • K. Matsuuchi, K. Ohnishi, T. Iino, T. Kuroiwa
    1993 Volume 58 Issue 1 Pages 33-39
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    We adopted epifluorescent microscopy to observe plasmid DNA in situ using Escherichia coli strain EJ812 (parCt8) as the host cell. In this strain abnormal chromosome partition occurs in the restrictive temperature, so that only plasmid DNA exists without chromosome DNA in some cells. EJ812 was transformed by either pBR322 or pUC18. When the transformant cells were incubated for three hours at 42°C, about 30% of them became finally anucleate. When the anucleate cells separeted from the normal cells were stained with 4'6-diamino-2-phenylindole, a few minute granules with fluorescent reaction were observed in the anucleate cells. They are inferred to be the complex of catenated plasmid DNA caused by an effect of parC. One anucleate cell was estimated to contain between 30 and 140 copies of the DNA molecule.
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  • Rafael Villalobos-Pietrini, Ana Rosa Flores-Márquez, Sandra G&o ...
    1993 Volume 58 Issue 1 Pages 41-45
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    The clastogenic effect of two detergents one anionic (Roma) and another nonionic (Triton X-100), potassium dichromate, and various concentrations of the mixtures metal-detergents was analyzed in the meristematic cells of the root tips of Vicia faba. The chromium compound induced chromosomal aberrations as well as c-metaphases in similar frequencies as the mixture chromium- surfactants. The detergents did not produce cytogenetic disturbances, nor did they facilitate the clastogenic effects of chromium.
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  • T. Yabuya, Y. Aiko, T. Adachi
    1993 Volume 58 Issue 1 Pages 47-51
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    To clarify the factors affecting the velvety outer perianths of Iris ensata, anthocyanins of the perianths and morphology of their epidermis were examined. The studied 2 velvety and 5 non-velvety cultivars were classified in order of major anthocyanins contained: malvidin 3RGac5G-petunidin 3RGac5G and petunidin 3RGac5G-malvidin 3RGac5G. However, there was no relationship between the velvety expression and the type of major anthocyanins. Also, the cultivars have 3 types concerning the perianth epidermal cells such as nipple, round and the intermediate types, and the nipple type does not necessarily induce the velvety perianths. As the results, the correlation of high anthocyanin contents, longer size and high density of epidermal cells of the perianthis was responsible for the velvety expression.
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  • Ahmed E. Yaseen
    1993 Volume 58 Issue 1 Pages 53-60
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Species of the qenus Bulinus occur as a polyploid series which is of interest because of the rarity of polyploidy in the animal kingdom and because it appears that there is some kind of relationship between polyploidy and the ability of this genus to transmit schistosomiasis, a parasitic disease of a major public health importance in most developing coutries. Chromosome analyses were performed in preparations of gonads, gills and embryos, from a hunded specimens of Bulinus truncatus which were collected from different localities around Qena City. The diploid chromosome number was determined to be seventy two (2n=72). The karyotype was made up of seventeen metacentric, thirteen submetacentric, three subtelocentric and three telocentric chromosome pairs. Considering previously reported cytogenetic data in the genus Bulinus, the present specimene appear to represent a tetraploid form and this is the first report in which detailed karyotype for Bulinus truncatus is presented. Habitat, morphological characters and shell measurements for this species are also described in detail in this paper.
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  • Saeed Ahmad
    1993 Volume 58 Issue 1 Pages 61-65
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Dry dormant seeds of two cultivars i.e., CSIMF and FlO of Cicer arietinum L. were irradiated with gamma rays. The doses were 20, 35, 60, 75, 110 and 150 KR. Meiotic studies were carried out in the control as well as in irradiated material. The meiotic abnormalities observed were stickiness of chromosomes, univalents, multivalents, Anaphase-I bridges, laggards and unequal segregation of chromosomes. Multivalent formation at metaphase-I and bridge formation at anaphase-i were observed in maximum cells. In general the meiotic abnormalities increased with the increase of radiation dose in both cultivars. However, cultivar F10 showed more chromosomal abnormalities as compared to cultivar CSIMF at the same dose.
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  • Male sex specific dys-synapsis
    M. L. H. Kaul, C. Nirmala
    1993 Volume 58 Issue 1 Pages 67-76
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Dys-synapsis, involving lack or impaired synaptic pairing, confined only to the male sex was detected in a 0.05% DES induced mutant of Pisum sativum variety Arkel. This anomaly is controlled by a single nuclear recessive gene msg4, non-allelic to the other msg genes isolated in P. sativum genome. The synaptic anomaly leads to abnormal male meiosis involving premature chiasmata terminalization, nucleolar multiplication, univalency, unequal and ir-regular chromosome disjunction at AI and AII, unequal triads and tetrads and coenocyth formations. This result in degenerated microspore formation rendering the mutant tota male sterile.
    The meiotic anomalies exhibit high proportion of variance and the initial anomalies add to the variance of the subsequent abnormalities making male meiosis exceedingly erratic. The major meiotic anomalies are inter-correlated but only some exhibit genetic correlations whicl unravel causes and consequences of meiotic anomalies detected in this mutant. The dys gene causing the male sex specific anomalies, does not belong to the gene system regulating chiasma formation and its terminalization in P. sativum. Instead, it is a special gene disrupting male meiosis only and is anther specific.
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  • Toshisuke Hiraoka, Yuichi Fuchikawa
    1993 Volume 58 Issue 1 Pages 77-84
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    In the Equisetum SMC, two kinds of poles, the PP and the MP, are established prior to and during the synaptic stage. They stand against each other on just opposite sides across the nucleus. These poles are formed in sequence, the former pole preceding the latter. The essental nature of the former is to attract plastids to form the PP on the BA side, while that of the latter to attract mitochondria to form the MP on the BB side of cytoplasm. These synaptic poles play a rôle in determining the prospective bouquet axis.
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  • G. García, E. Scvortzoff, M. C. Máspoli, R. Vaz-Ferreira
    1993 Volume 58 Issue 1 Pages 85-94
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    High levels of variation in chromosome numbers and N. F. s from 48-80. C-banding analysis allowed us to detect C-positive regions in three positions: centromeric, telomeric and interstitial. A differential C-banding pattern was found in each species. These banding patterns can be interpreted as a result of namely pericentric inversions and centric fusions occuring alternatively during the karyotypic reorganization of these species.
    The karyotypic analysis of 17 specimens of Cynolebias using silver stain NOR technique showed 3-6 rDNA active regions among these species. A reduction in the number or expression of NORs occurred in the species with lower chromosome number as a result of centric fusions. Terminal active NORs seem to be the most common situation among these species. Other positions of the active rDNA cistrons can be explained through chromosomal rearrangements.
    All present data are consistent with Scheel's hypothesis that pericentric inversions and centric fusions occurred in the karyotypic reorganization in the Rivulinae.
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  • Yoshikane Iwatsubo, Naohiro Naruhashi
    1993 Volume 58 Issue 1 Pages 95-98
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    The chromosome number and karyotype of two Japanese Stephanandra species, S. incisa and S. tanakae, were investigated. Both species had the same chromosome counts of n=9 and 2n=18, which showed that the basic chromosome number of the genus was x=9. The two species had similar karyotypes, with 7 metacentric pairs, one of which had a satellite, and 2 submetacentric pairs.
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  • Kohji Hasunuma, Kazushi Oda
    1993 Volume 58 Issue 1 Pages 99-105
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Diphtheria toxin with the molecular mass of 60 kDa was detected to be capable of binding [α-32P] ATP, [α-32P] GTP and [α-32P] UTP. The binding of [α-32P] ATP was prevented in the presence of 10-3 M ATP or GTP and that of [α-32P]GTP was completely prevented in the presence of 10-4 M ATP, GTP or UTP. CTP at a concentration of 10-4 M stimulated the binding of the above three labeled nucleotides. Diphtheria toxin was auto-[32P]ADP-ribosylated. This was prevented in the presence of 10-3 M ATP, GTP or UTP. At 10-5 M NAD was stimulated the binding of [α-32P] ATP and [α-32P] GTP but at 10-5 M it inhibited it. UV-A light irradiation stimulated the binding of [α-32P]ATP and [α-32P] GTP to 58 kDa and 77 kDa, and [α-32P]GTP to 83 kDa and 129 kDa proteins in the microsomal fraction of band strain of Neurospora crassa. NAD at 10-5 M stimulated the binding of [α-32P] ATP to 58 kDa and 77 kDa proteins. In the case of [α-32P] GTP, 10-5 M NAD reduced the back ground binding of [α-32P] GTP, and further the presence of diphtheria toxin reduced the background binding of [α-32P] GTP to these four proteins. UV-A light stimulated the binding of [α-32P] ATP and [α-32P] GTP to diphtheria toxin in the presence of the microsomal fraction.
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  • Kohji Hasunuma, Kazushi Oda, Tohru Hamada
    1993 Volume 58 Issue 1 Pages 107-114
    Published: March 25, 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
    Cholera toxin is composed of a catalytic subunit (29 kDa) called the A protomer and binding subunits (pentamer of 11.6 kDa subunit) called B oligomer. The binding of [α-32P] ATP, [α-32P] GTP, [α-32P] CTP and [α-32P] UTP at 1.6×10-7 M was detected in the binding subunits, in which the binding of [α-32P] GTP was most efficient. The binding of [α-32P] ATP to the binding subunits was stimulated in the presence of 10-4 M ATP, GTP, CTP or UTP. The binding of [α-32P] GTP was strongly inhibited in the presence of 10-3 M ATP, GTP or UTP. Similar results were observed in the binding of [α-32P] CTP. The binding of [α-32P] UTP was strongly inhibited in the presence of 10-4 M ATP, GTP, CTP or UTP. The binding of [α-32P] GTP to the B oligomer was stimulated in the presence of the A protomer. The [32P] ADP-ribosylation of the A protomer (29 kDa) and A-2 protomer (24 kDa) lacking A-1 (5 kDa) was unaffected by ATP, GTP, CTP or UTP at a concentration of 10-4 or 10-3 M. At a concentration of 10-4 M NAD stimulated the binding of [α-32P] ATP and [α-32P] GTP but at a concentration of 10-3 M it inhibited it.
    The plasma membrane was purified from the red light irradiated third internode of etiolated pea seedlings. The purified plasma membrane was mixed with cholera toxin in the presence of 1.6×10-7 M [α-32P] ATP, [α-32P] GTP, [α-32P] CTP or [α-32P] UTP. The binding of [α-32P] GTP was stimulated partly proportional to the fluence rates of red light irradiation of the third internodes.
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  • 1993 Volume 58 Issue 1 Pages e1
    Published: 1993
    Released: March 19, 2009
    JOURNAL FREE ACCESS
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