The present study was carried out to evaluate the cytogenetic effect of manganese sulfate (MnSO
4) in cultured mouse spleen cells and the possible protective effect of folic acid (FA) and/or vitamin B
12 (VB
12) on cell cultures treated with MnSO
4. Cultured mouse spleen cells were treated with MnSO
4 at 4 concentrations (10
-6, 5×10
-6, 10
-5, 10
-4). FA at 10
-5, 5×10
-5 and 10
-4M, and VB
12 at 5×10
-6, 10
-5 and 5×10
-5 M were used for testing the protective effect on chromosome damage induced by MnSO
4. The highest dose (10
-4 M) of MnSO
4 were treated simultaneously with FA and/or VB
12. The cultures were set up for 24 h. The results indicate that MnSO
4 alone induced a concentration-related increase in the percentage of chromosome aberrations. Both FA and VB
12 reduced the percentage of aberrant cells in a significant (p<0.01) and dose-dependent manner. However, FA combined with VB
12 caused a highest percentage of reduction (62.20-71.94%). Under the present experimental conditions, FA and VB
12 are capable of reducing the cytogenetic damage induced by MnSO
4 and appear to be an antimutagenic agent.
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